text.skipToContent text.skipToNavigation

miRNeasy Micro Kit

用于从少量细胞核组织中纯化miRNA和总RNA
  • 从小样本中有效纯化miRNA和总RNA
  • 高纯度RNA,适用于多种下游应用
  • 简单、灵敏的操作流程
  • 自动化实验方案

miRNeasy Micro Kit用于从少量培养细胞、分选细胞、冰冻切片激光捕获显微切割(LCM)样本和动物及人体组织中纯化RNA,包括miRNA和其他小RNA分子。

Buy Products

Cat No./ID: 217084
miRNeasy Micro Kit (50)
For 50 total RNA preps: 50 RNeasy MinElute Spin Columns, Collection Tubes (1.5 ml and 2 ml), QIAzol Lysis Reagent, RNase-Free Reagents and Buffers

miRNeasy Micro Kit适用于分子生物学应用。该产品不适用于疾病的诊断、预防或治疗。


Product Details

9
miRNeasy Procedures.

The miRNeasy Mini Kit enables purification of total RNA which includes RNA from 18 nucleotides (nt) upwards. Alternatively, an miRNA-enriched fraction and a total RNA (>200 nt) fraction can be can be purified separately. A separate miRNA-enriched fraction may be required to reduce background in particularly sensitive downstream applications.

9

High, reproducible recovery of miRNA from small cell numbers.

Total RNA was purified from 10-fold serial dilutions of Jurkat cells (106 to 10 cells) using the miRNeasy Micro Kit. RNA was purified from 2 replicates of each dilution, either [A] manually or [B] using the QIAcube. Purified RNA was used to detect miR-16 using the miScript PCR System on a Rotor-Gene Q cycler. miR-16 was detected in as few as 10 cells and CT values were consistently reproducible. No signal was detected in negative controls (blue and yellow curves).

Performance
miRNeasy Micro Kit可有效纯化来自少量组织和细胞样本的RNA(参见"High, reproducible recovery of miRNA from small cell numbers")。可纯化多种组织和细胞的RNA,包括难裂解组织。

miRNeasy Kits制备的RNA纯度高,可直接用于敏感的下游应用。miRNeasy操作流程将盐或苯酚污染的可能性降到最低,这些污染可能影响后续分析。与其他miRNA纯化(如使用苯酚抽提和沉淀)相比,miRNeasy Micro Kit除了纯度更高以外,还能获得更高产量。
Principle

miRNeasy Micro Kit可从少量培养细胞(至多1 x 106个细胞)和多种动物及人体组织(至多5 mg组织)中纯化总RNA,包括miRNA和其他小RNA分子。试剂盒整合了基于苯酚/胍的样本裂解方法和基于硅胶膜的总RNA纯化方法。

试剂盒中包括的QIAzol Lysis Reagent是苯酚和异硫氰酸胍的单相溶液,便于裂解组织,抑制RNases,并去除大部分有机抽提裂解液中的胞内DNA和蛋白。样本在QIAzol Lysis Reagent中匀浆后,加入氯仿并离心,RNA位于上层水相。抽提水相,在RNeasy MinElute离心柱上纯化RNA,污染物被洗去。独特设计的RNeasy MinElute离心柱可用少至10 µl洗脱体积浓缩RNA。

Procedure

miRNeasy Micro Kit纯化的总RNA包括18个核苷酸以上的RNA。富含miRNA的组分和总RNA(>200 nt)组分可分开纯化(参见"miRNeasy Procedures")。包括miRNA的总RNA非常适合通过real-time RT-PCR的miRNA定量分析。单独富含小RNA的组分更适用于某些mRNA和rRNA会导致更高背景的应用。

在QIAcube全自动核酸纯化仪上自动纯化miRNA

在QIAcube全自动核酸纯化仪上可完全自动纯化包括miRNA的总RNA(参见"High, reproducible recovery of miRNA from small cell numbers")。新型QIAcube全自动核酸纯化仪采用先进技术处理QIAGEN离心柱,将自动化、低通量样品制备无缝整合到实验室工作流程中。使用QIAcube全自动核酸纯化仪的样品制备流程与手工操作相同(如裂解、结合、洗涤和洗脱),可使用miRNeasy Micro Kit进一步纯化高品质RNA。

Applications

miRNeasy Micro Kit可纯化包括miRNA的总RNA,用于多种应用,包括:

  • Northern印迹分析
  • 定量real-time RT-PCR(如使用miScript PCR System)
  • 芯片分析

Product Resources

您无权限查看此资源

学术海报 (1)
fragment fix placeholder