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PAXgene Blood DNA Kit

从全血样本中纯化至多500 μg基因组DNA

  • 同一体系中进行血液收集和纯化
  • 易于操作
  • 提高工作流程的效率

PAXgene Blood DNA系统包括用于血液采集和稳定的PAXgene Blood DNA Tubes和用于单管纯化DNA的PAXgene Blood DNA Kit。

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Cat No./ID: 761133
PAXgene Blood DNA Kit (25)
Processing tubes and buffers for 25 preparations

仅限于科研应用,不适用于诊断,不为疾病的诊断、预防或治疗提供参考信息。


Product Details

9
High quality and high molecular weight of genomic DNA.
Genomic DNA isolated from 8 blood donors using the PAXgene Blood DNA System. [A] Agarose gel analysis; [B] pulsed-field gel electrophoresis for enhanced separation of high-molecular-weight genomic DNA.  M: markers.
9
Efficient multiplex PCR of 3 mitochondrial genes.
Multiplex PCR of fragments from the mitochondrial genes tRNAlys/ATPase (0.92 kb), tRNAleu(UUR) (0.63 kb), and ND4 (0.29 kb), using 250 ng DNA from 8 donors as starting material.
9
PAXgene Blood DNA procedure.
Blood samples (8.5 ml) are collected in PAXgene Blood DNA Tubes, where they may be stored or transported. For DNA isolation, the blood is transferred to processing tubes (supplied already filled with cell lysis buffer), and the solution is mixed to lyse red and white blood cells. Cell nuclei and mitochondria are pelleted by centrifugation, washed, and resuspended in digestion buffer. Protein contaminants are removed by incubation with a protease. DNA is precipitated in isopropanol, washed in 70% ethanol, dried, and resuspended in resuspension buffer.
Performance
PAXgene Blood DNA System分离的DNA A260/A280比值在1.7–1.9。片段大小20–200 kb,平均长度50–150 kb(参见"High quality and high molecular weight of genomic DNA")。DNA平均产量为150–500 µg,由血样中的核细胞数目决定。纯化的DNA可用于PCR、定量和real-time PCR(参见"Efficient multiplex PCR of 3 mitochondrial genes")。
Principle

PAXgene Blood DNA System是整合的标准化体系,用于收集和稳定全血样本并分离基因组DNA。该体系通过PAXgene Blood DNA Tubes收集和稳定血液,并使用PAXgene Blood DNA Kit纯化DNA。

PAXgene Blood DNA Tubes包含可防止血液凝结以及可稳定白细胞的混合试剂,用来收集血液。血液样本可在室温下在管中储存长达14天。用PAXgene Blood DNA Kit中提供的缓冲液获得的高纯度的基因组DNA适合各种下游应用。

Procedure

PAXgene Blood DNA操作步骤如流程图"PAXgene Blood DNA procedure"所示。血液样本(8.5 ml)由PAXgene Blood DNA Tubes收集,并储存或运输。如需纯化DNA,则将血液转入处理管中(已预充细胞裂解缓冲液),将溶液混合,裂解红细胞和白细胞。细胞核和线粒体通过离心收集、洗涤,重悬于裂解缓冲液中。蛋白污染物经蛋白酶孵育后去除。DNA用异丙酮沉淀、70%乙醇洗涤、干燥,并重悬于缓冲液。

Applications

纯化的DNA可用于多种下游应用,包括:

  • PCR和定量real-time PCR 
  • Southern印迹
  • SNP基因分型
  • 药物基因组研究
  • SNP发现和鉴定

Specifications

Features
Specifications
应用 PCR, qPCR, real-time PCR, blotting, genotyping
洗脱体积 1 ml
规格 Processing tube
主要样本类型 Whole blood
处理 Manual (centrifugation)
样本量 8.5 ml
Time per run 60 min/8 samples
产量 150–500 µg

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产品介绍与指南 (3)
Second edition — innovative tools
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For collection, storage, and transport of whole blood for genomic DNA isolation

 


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常见问题 (18)
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试剂盒操作手册 (1)
For isolation of genomic DNA from 8.5 ml human whole blood
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