The miScript Plant PCR System enables sensitive, specific miRNA quantification and profiling using SYBR Green real-time PCR. The miScript Plant PCR System covers all the steps involved in conversion of RNA to cDNA and subsequent real-time PCR detection of miRNAs.
Mature miRNA profiling with the miScript PCR System uses the following components:
miScript Plant RT Kit – Built specifically for plant samples. This kit enables simple, single-step cDNA synthesis. A single cDNA synthesis reaction can be used for detection of hundreds of miRNAs. The dual buffer system meets the distinctive needs of miRNA quantification using real-time PCR.
miScript PreAMP PCR Kit and Primer Mixes – This kit enables an optional preamplification step when working with limited RNA amounts.
miScript SYBR Green PCR Kit –This kit includes QuantiTect SYBR Green PCR Master Mix and the miScript Universal Primer, a reverse primer that allows detection of miRNAs in combination with a miScript Primer Assay or miScript miRNA PCR Array.
miScript miRNA PCR Array – Cutting-edge miScript miRNA Plant PCR Arrays consist of mature miRNA-specific forward primers (miScript Primer Assays) arrayed in biologically relevant panels or miRNome arrays.
The exclusive, scalable PCR array sets enable you to profile the relative expression of corn, rice or soybean miRNAs:
miScript Corn miRBase Profiler – Profile up to 168 corn miRNAs; the 2-plate set contains 84 miScript Primer Assays on each plate
miScript Rice miRBase Profiler – Profile up to 504 rice miRNAs; the 6-plate set contains 84 miScript Primer Assays on each plate
miScript Soybean miRBase Profiler – Profile up to 336 soybean miRNAs; the 4-plate set contains 84 miScript Primer Assays on each plate
Each array set enables the accurate relative quantification of the most current corn, rice or soybean miRNA genome (miRNome or microRNome) as annotated in miRBase (www.miRBase.org). Individual plate(s) containing miRNAs of interest may be selected, or plates may be used in succession to profile miRNAs from each miRBase release up through the most recent version (currently V20). The flexibility and completeness of the panel ensures that scientists can identify all of the miRNAs that potentially impact a specific biological process. A set of controls present on this array enables data analysis using the ΔΔCT method of relative quantification, assessment of ligation-mediated reverse-transcription performance, assessment of reverse-transcription performance, and assessment of PCR performance. This unique series of PCR arrays delivers the specificity, sensitivity, and scalability to support any miRNA expression research.
miScript miRNA PCR Arrays are intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease. miRBase Profiler is not associated with miRBase or miRBase.org or the University of Manchester, the BBSRC, the Wellcome Trust, or the Sanger Institute.