Housekeeping Genes

RT² Profiler Array Housekeeping Genes PCR Arrays are used to screen for potential normalization genes for use in real-time PCR analysis. Housekeeping genes encode proteins that are usually essential for the maintenance of cellular function and often remains constant under most experimental conditions. With this array, you can easily profile the expression of twelve (12) commonly used housekeeping genes in eight (8) samples using SYBR-green based qPCR.

A guaranteed system

RT² Profiler PCR Arrays are tested and optimized in combination with the RT² SYBR^® Green qPCR Mastermixes and the RT² First Strand Kit. This testing means that RT² Profiler PCR Array performance is guaranteed when all three of these components are used together.

Sensitivity

When using the RT² First Strand Kit, start with as little as 1 ng or as much as 5 µg of total RNA per sample.

Reproducibility

The complete PCR array system demonstrates strong correlations across technical replicates, lots, and instruments with average correlation coefficients >0.99, ensuring reliable detection of differences in expression between biological samples.

Specificity

The PCR array system, with high-quality input RNA, yields single bands of the predicted size without primer-dimers or other secondary products, therefore providing the highly accurate real-time PCR results.

Uniform PCR amplification efficiency

Uniform PCR amplification efficiency is required for the PCR array technology to allow accurate comparisons of gene expression across all genes and all samples. The unique combination of our proprietary primer design algorithm and rigorous testing of every primer assay guarantees the high performance of every primer assay on PCR arrays.

RT² Profiler PCR Arrays are reliable tools for analyzing the expression of a focused panel of genes. Each 96-well plate, 384-well plate or 100-well disc PCR array includes SYBR Green-optimized primer assays for a thoroughly researched panel of relevant, pathway- or disease-focused genes. RT² Profiler PCR Arrays can also be customized to contain a panel of genes tailored to your specific research interests. The high-quality primer design and RT² SYBR Green qPCR Mastermix formulation enable the PCR array to amplify 96 or 384 different gene-specific products simultaneously under uniform cycling conditions.

This combination provides the RT² Profiler PCR Array with the specificity and the high amplification efficiencies required for accurate real-time SYBR Green results. PCR arrays are easy to use in any research laboratory.

Simply mix the cDNA template with the appropriate ready-to-use PCR mastermix, aliquot equal volumes to each well of the same plate, and then run the real-time PCR cycling program. RT² Profiler PCR Arrays are compatible with all QIAGEN, ABI, Bio-Rad, Eppendorf, Roche, and Stratagene instruments.

Flexible layout and controls

RT² Profiler PCR Arrays are available in 96-well plate, 384-well plate, and 100-well disc formats, and are used to monitor the expression of 84 or 370 genes related to a disease state or pathway, plus 5 housekeeping genes. Each RT² Profiler PCR Array also includes control elements for:

• Data normalization
• Genomic DNA contamination detection
• RNA sample quality
• General PCR performance

Easy-to-use data analysis

Data can be analyzed using an easy-to-use Excel-based data analysis template or Web-based software. Data analysis is based on the ΔΔC_T method with normalization of the raw data to either housekeeping genes.

RT² PCR Profiler Arrays can be used in all areas of biological and medical research, including:

• Cancer Inflammation and cytokine profiling
• Stem cells
• Neuroscience
• Signal transduction pathways
• Cell adhesion and cell migration
• Biomarker screening and Validation