QIAxcel Advanced System
便利的DNA片段和RNA分析
Features
- 快速分析多达96个样本，无需人工介入
- 采用安全、操作简单的即用型预制胶
- 可检测到浓度低至0.1 ng/µl的核酸，结果可靠
- 标准化、准确的分析，分辨率可达3–5 bp
- 界面友好的分析软件，支持21 CFR part 11规章
✓ 24/7 automatic processing of online orders
✓ Knowledgeable and professional Product & Technical Support
✓ Fast and reliable (re)-ordering
QIAxcel Advanced Instrument
Cat. No. / ID: 9001941
Capillary electrophoresis device: includes computer, QIAxcel ScreenGel Software, and 1-year warranty on parts and labor
InstrumentSoftwareProduct
QIAxcel Advanced
QIAxcel ScreenGel Software
Service Options
Warranty
System
Priority
PrioPLUS
✓ 24/7 automatic processing of online orders
✓ Knowledgeable and professional Product & Technical Support
✓ Fast and reliable (re)-ordering
Product Details
新颖的QIAxcel Advanced体系取代了传统的、费力的DNA和RNA胶分析，流线式工作流程，节省时间。QIAxcel Advanced体系是灵敏的、高分辨率的毛细管电泳系统，每次能全自动分析多达96个样本。可在3分钟内完成12个样本的DNA片段分析。即用型预制胶分析96个样本，使用最少的转运时间，减少手动操作的错误，无需繁琐的制胶过程。界面友好的QIAxcel ScreenGel软件简化了分析和数据采集。
Performance
简化的工作流程
QIAxcel Advanced体系无需平板胶分析过程，加快了DNA和RNA分析流程，并且简化了整个样本纯化和分析过程（参见" Streamlined workflow"）。配合经验证的QIAGEN终点PCR试剂盒，QIAxcel Advanced体系提供一体化的、预先测试的方案，用于可靠地分析PCR片段，重复性好并有效的节省时间和经费。
一个仪器，多种应用
QIAxcel Advanced体系是多重电泳系统，适合多种应用。预设的方法配合相应的预制胶，可分离和分析多种核酸，包括单个和多重PCR片段、限制性内切酶消化的DNA、总RNA和cRNA。用于基因分型时，与传统的琼脂糖凝胶电泳相比，QIAxcel Advanced体系能够更加准确和灵敏的检测（参见" Accurate, high-throughput genotyping of bacteria"）。QIAxcel Advanced体系的高通量能力使其非常适合使用96孔纯化技术的实验室，需要一个快速的解决方案用于进行质量控制（参见" Analysis of purified RNA"）。不同于其他只能同时处理12个样本的仪器，QIAxcel Advanced体系能够同时处理96个样本，而无需手工介入。
See figures
Principle
QIAxcel Advanced系统包括一系列激光二极管和内置QIAxcel预制胶卡夹的微光采集器。片段在胶中移动的过程中毛细管旁路激发并检测信号，信号通过光电倍增管传至可进行数据分析的QIAxcel ScreenGel软件（参见" Sample separation process"）。
QIAxcel Advanced体系可进行高灵敏度的检测，即使分析低浓度核酸也可获得可靠的结果。小于0.5 kb的片段分辨率为3–5 bp，相比平板胶法QIAxcel Advanced体系确保更高的精确度，同时提供更可靠的数据分析。每次分析样本消耗量少于0.1 μl，为下游应用节约了珍惜样本。全自动上样和自控组件将接触如溴化乙锭等危险化学品的风险率降至最低（参见" Ready-to-run gel cartridges"）。
一系列QIAxcel Kits适合多种下游应用，满足不同的分辨率和速度需求（见表）。
| QIAxcel Kit | 被分析物 | 片段范围 | 100 bp – 500 bp* | 500 bp – 1 kb* | 1 kb – 5 kb* | 5 kb – 10 kb* | 运行时间/12个样本† |
|---|---|---|---|---|---|---|---|
| QIAxcel DNA High Resolution Kit | DNA | 15 bp – 10 kb | 3–5 bp | 50 bp | 200–500 bp | 1–1.5 kb | 7–20 min |
| QIAxcel DNA Screening Kit | DNA | 15 bp – 5 kb | 20–50 bp | 50–100 bp | 500 bp | – | 5 min |
| QIAxcel DNA Fast Analysis Kit | DNA | 15 bp – 3 kb | 50–100 bp | 100–250 bp | 250 bp – 1 kb‡ | – | 3–5 min |
| QIAxcel RNA QC Kit v2.0 | RNA | 15 b – 6 kb | – | – | – | – | 10 min |
See figures
Procedure
QIAxcel Advanced体系只需简单的几步操作：预制胶卡夹、预装的缓冲液槽及装载有样本的96孔板或PCR管或联管、选择分离方法。无需冗长的制胶及用户培训，简化了日常工作的整个流程。运行开始几分钟内，会在电脑屏幕上实时出现第一个结果（参见" Reduced run times"）。
See figures
Applications
QIAxcel Advanced体系提供多种优化的应用，非常适合学术研究以及药学、生物技术和生物化学实验室。预设的方法配合相应的预制胶，可分离和分析多种核酸，包括单个和多重PCR片段、限制性内切酶消化的DNA、合成的寡聚核苷酸、总RNA和cRNA。
Software
QIAxcel ScreenGel软件，更加方便
QIAxcel ScreenGel软件专为QIAxcel Advanced体系设计，是功能强大、界面友好的软件，用于数据采集和分析。可通过电泳图谱和胶图两种方式查看数据，交互式工具简化了分析，便于快速解释数据。可单独或同时查看结果，用于对比样本或数据。对于多个数据进行一体化的分析方式简化了评估过程，独特的数学计算法则可以表格形式显示各种峰的特性，包括峰的数目、高度、宽度和每个峰的面积。可简单生成数据对比报表，保存并输出满足需要的文档（参见" Simplified and standardized data collection and analysis"）。
易于安装和启动
Process Wizard简化了实验启动过程（参见" Easy-to-use software features"）。这一方便的特性可引导安装、获得较好的运行参数和选择DNA大小标记。也包含试剂的批号信息。只需单击几下鼠标即可选择样本，且运行检查也很容易。
支持21 CFR Part 11 compliance
QIAxcel ScreenGel软件支持21 CFR Part 11规章的技术要求。安全特性包括：
- 输入密码登陆，防止未授权访问和数据处理
- 审查跟踪配置文件和系统事件
- 自动保存和存档写保护的原始数据
- 安全的用户管理
不同的用户配置文件均可（Routine、Basic、Advanced和Admin），且密码保护（需用户登陆）提高安全性。简单的检索界面，用户无需培训，对于没有经验的使用者该软件特别具备吸引力。
See figures
Services
Have your throughput demands increased? Do you want to expand your application range or further streamline workflows? Do you need to manage more complex samples? Do you need improved connectivity?
If the answer to any of these questions is yes, then QIAGEN’s trade-in/trade-up program is just right for you!
QIAGEN makes it easy to keep up to speed with the latest automation and sample processing technologies. Simply exchange an old instrument for a new one. Improve your efficiency by trading in a competitor instrument or trading up with an older QIAGEN instrument.
Learn more about trade-in/trade-up opportunities.
Supporting data and figures
Assessment of DNA degraded by sonication.
[A] Agarose gel photo showing gDNA in various states of degradation. Lane 1: Non-degraded gDNA samples; lanes 2 and 3: gDNA samples partially degraded by sonication with 7 and 14 pulses, respectively; lanes 4 and 5: gDNA samples fully degraded with 70 and 105 pulses, respectively. [B] QIAxcel gel photo showing gDNA samples in three states of degradation. 1, 3, and 5 correspond to lanes 1, 3, and 5 in [A]. [C] Electropherograms and a gel photo of gDNA samples from lanes 1, 3, and 5 in [A]. Lane 1: Electropherogram indicates very good quality gDNA (no degradation products before the major peak, long tailing off); lane 3: partially degraded gDNA (some degradation product signals, no tailing off); lane 5: highly degraded gDNA (many degradation product signals, no tailing off).
Service Plans
QIAxcel Advanced System PRV-2
Cat. No. / ID: 9002874
Instrument and service agreement package: workstation for automated capillary electrophoresis, notebook computer and QIAxcel software; includes installation, application training and two inspection service visits. One-year warranty on labor and parts are also included. Instrument is serviced at our Regional Repair Center.
QIAxcel Advanced, PrioPLUS, no PM
Cat. No. / ID: 9002200
Instrument and service agreement package: workstation for automated capillary electrophoresis, notebook computer and QIAxcel software; includes installation, application training and Full Agreement for three years. Instrument repaired in Regional Repair Center with cost of labor, shipping and parts covered. Does not include preventive maintenance.
QIAxcel, Full Agreement
Cat. No. / ID: 9242699
Instrument repair service for the QIAxcel at regional repair center. Shipping, labor and parts included during the Full Agreement period. Loaner instrument provided within 2–3 business days. Instrument repair turnaround time of 7–10 business days. Includes one on-site Preventive Maintenance or Inspection Service during the Full Agreement period.
QIAxcel Advanced, Core Agreement
Cat. No. / ID: 9245252
One-time QIAxcel Advanced instrument repair performed at a regional repair center including shipping, labor, and spare parts for a period of one year. Instrument repair turnaround time of 7–10 business days. Includes one on-site Preventive Maintenance or Inspection Service and a 10% discount on additional repair service during the Core Agreement period.
QIAxcel, Preventive Subscription
Cat. No. / ID: 9241224A
One on-site Preventive Maintenance or Inspection Service visit for the QIAxcel, including travel, labor and parts. Includes a 10% discount on repair services during the Preventive Subscription period.
Resources
试剂盒操作手册 (8)
操作软件 (4)
产品介绍与指南 (9)
其他资源 (8)
应用说明 (33)
安全数据表 (1)
学术海报 (7)
补充实验方案 (1)
用户使用手册 (1)
仪器技术参数 (2)
白皮书 (3)
FAQ
If a different method is used with the QIAxcel System or QIAxcel Advanced, does a new reference marker table need to be created?
When running RNA samples using the QIAxcel System or QIAxcel Advanced, are there any special things to consider?
What to do if alignment marker and samples are migrating too slowly and the upper alignment marker does not appear on the gel- and/or electropherogram view with the QIAxcel System or QIAxcel Advanced?
How can any clogged capillaries be cleared for use with the QIAxcel System and QIAxcel Advanced?
Why does an intensity calibration of the gel cartridge for the QIAxcel System and QIAxcel Advanced need to be performed?
Why does the Power LED not function with the QIAxcel or QIAxcel Advanced instrument plugged in and the main power switch turned on?
What can be done if lower and upper alignment markers used with the QIAxcel System or QIAxcel Advanced are not aligned correctly?
If a different DNA size and/or alignment marker is to be used with the QIAxcel System or QIAxcel Advanced, does the Gel Cartridge need to be recalibrated?
What is the QIAxcel Advanced System?
Why do DNA samples appear in only a few channels when the alignment marker is working using the QIAxcel System or QIAxcel Advanced?
Why are there no peaks in all channels during a run with the QIAxcel System or QIAxcel Advanced??
Why is the alignment marker for the 1.8 kb fragment not visible when using the 25 bp/1.8 kb DNA Size Marker together with the 15 bp/400 bp marker on the QIAxcel System and QIAxcel Advanced?
When should the N2 bottle used with the QIAxcel Advanced System be changed?
How often should the solutions in the Buffer Tray for the QIAxcel System or QIAxcel Advanced be replaced?
How can drying out of the gel cartridge tips used on the QIAxcel System and QIAxcel Advanced be prevented?
What is the minimum sample volume required for the QIAxcel System and QIAxcel Advanced?
How can a clogged capillary channel be identified when running samples on the QIAxcel System or QIAxcel Advanced?
Can the QIAxcel System or QIAxcel Advanced be connected to a network?
Why are PCR bands relatively weak and "smearing" but the alignment marker bands are sharp when running samples on the QIAxcel System and QIAxcel Advanced?