GeneGlobe Analyze solutions combine industry-leading algorithms and knowledge with ease-of-use. The cloud-based QIAGEN RNA-seq Analysis Portal is accessible from GeneGlobe Analyze and enables analysis of RNA-seq data, from alignment of raw reads to interpretation of differentially expressed genes and finding the right tools for verification of potential biomarkers.
The QIAGEN RNA-seq Analysis Portal cuts down analysis time from days to just a few hours:
The QIAGEN RNA-seq Analysis Portal benefits your research in many ways:
The portal's user-friendly project interface allows data upload and setup of new experiments in just three easy steps (see figure GeneGlobe RNA-seq Analysis Portal projects overview). After alignment, gene counts are generated, and QIAGEN CLC Genomics Workbench algorithms are leveraged to perform differential gene expression analysis based on user-defined experiment groups.
The set of differentially expressed genes is evaluated for its likely impact on canonical pathways, diseases and functions, and upstream regulators driving the expression of these genes are predicted (see figure Interactive results dashboard). The portal displays the top ten of each type from the QIAGEN Ingenuity Pathway Analysis (IPA) Knowledge Base. A full license for QIAGEN IPA is needed to view and explore the complete set of biological interpretations.
Sample and quality control include the experimental setup and design, overview of samples in the experiment and commonly used QC metrics like read trimming and mapping statistics, principal component analysis and biotype distribution (see figure QC including RNA biotype distribution).
The results dashboard directly interfaces with QIAGEN's GeneGlobe Design & Analysis Hub, where differentially expressed genes can be explored and filtered based on p-value and fold change and saved to your personal My Projects space in My GeneGlobe. The right tools are also recommended for biomarker verification experiments (see figure Products for biomarker verification in GeneGlobe), and the saved genes or miRNAs of interest can be further explored in the context of pathways, functions and publications (see figure Pathway maps in GeneGlobe).
For more information on the principle and technical background, refer to the RNA-seq Analysis Portal User Manual.
For RNA-seq data analysis, including:
Supports the most commonly used RNA-seq library preps for Illumina instruments:
New England Biolabs:
Roche Sequencing solutions:
Thermo Fisher Scientific: