Easy-to-use AllPrep Bacterial/Fungal DNA/RNA/Protein Kits isolate total nucleic acids and cellular proteins from Gram (+/-) bacterial cultures and fungal cell cultures. The bead-beating method and spin column technology effectively extract nucleic acids and proteins in a streamlined process. DNA and RNA are ready for qPCR, RT-PCR and next-generation sequencing, while protein is ready for 1D SDS-PAGE and mass spectrometry following in-gel trypsin digestion. Some applications, including 2D SDS-PAGE and solution phase proteolytic digestion for mass spectrometry, may require an additional detergent removal step following protein elution.
Cultured bacterial and fungal cells are first lysed using glass bead tubes, and nucleic acids and proteins are solubilized and mixed with a binding solution. DNA binds to the first spin column and the flow-through is combined with a solution to bind the total RNA on a second spin column. The final flow-through captures protein. Each of the individual spin columns is then washed and the immobilized analyte is eluted.
|Features||Allprep Bacterial DNA/RNA/Protein Kit||Allprep Fungal DNA/RNA/Protein Kit|
|Bead size||0.1 mm glass||0.5 mm glass|
|Format||Silica Spin Filter||Silica Spin Filter|
|Processing||Chemical lysis and/or bead beating||Chemical lysis and/or bead beating|
|Sample size||1.8 ml of microbial culture QIAGEN recommends using no more than 1x109 bacterial cells per sample||1.8 ml of fungal culture|
|Sample types||Cultured Gram-negative and Gram-positive bacteria||Cultured fungi|
|Storage temperature||Store spin filters at 4°C. All other kit reagents and components can be stored at room temperature (15–30°C).||Store spin filters at 4°C. All other kit reagents and components can be stored at room temperature (15–30°C).|
|Throughput||1–24 samples||1–24 samples|
|Time per run or per prep||36 minutes||36 minutes|