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QIAamp DNA Micro Kit

从痕量样本中纯化基因组DNA和线粒体DNA

  • 快速纯化高品质DNA
  • 无需有机提取或乙醇沉淀
  • 重复性好,产量高
  • 完全去除污染物和抑制剂

QIAamp DNA Micro Kit使用快速离心柱流程,简化了基因组DNA和线粒体DNA的纯化过程。经优化的操作方法适用于痕量的新鲜或冷冻血液、组织和干血斑。试剂盒中的硅胶膜具有选择性结合的特性,以及20–100 μl的灵活洗脱体积。QIAamp DNA Micro Kit可在QIAcube全自动核酸纯化仪上自动纯化DNA。

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Cat No./ID: 56304
QIAamp DNA Micro Kit (50)
For 50 DNA preps: 50 QIAamp MinElute Columns, Proteinase K, Carrier RNA, Buffers, Collection Tubes (2 ml)

QIAamp DNA Micro Kit适用于分子生物学应用。该产品不适用于疾病的诊断、预防或治疗。


Product Details

9
Laser microdissection PCR.
DNA was purified from rodent kidney laser microdissection (LMD) samples using the QIAamp DNA Micro Kit or using a direct lysis PCR protocol, as indicated. GAPDH PCR was performed on 1 and 10 µl of each eluate. M: Marker; +: positive control; : negative control.
9
Efficient purification of DNA from small sample sizes.
DNA was purified from blood samples (164 µl) using the QIAamp DNA Micro Kit following the blood protocol. DNA was eluted in 20 µl Buffer AE and 2 µl of each eluate was amplified by real-time PCR. Efficient amplification was shown in all cases.
9
QIAamp DNA Micro procedure.

Optimized buffers lyse samples, stabilize nucleic acids, and enhance selective DNA adsorption to the QIAamp membrane. Alcohol is added and lysates loaded onto the QIAamp MinElute spin column. Wash buffers are used to remove impurities and pure, ready-to-use DNA is then eluted in water or low-salt buffer.

Performance
纯化的DNA不含蛋白质、核酸酶和其他杂质,适用于多种敏感的下游应用,如real-time PCR(参见"Efficient purification of DNA from small sample sizes")和激光显微切割(LMD)PCR(参见"Laser microdissection PCR")。QIAamp DNA Micro Kit纯化的DNA也适用于短重复序列(STR)和单核苷酸多态性(SNP)基因分型或药物基因组研究。
Principle

QIAamp DNA Micro Kit具备硅胶膜选择性结合DNA的特性,以及20 µl到100 µl的灵活洗脱体积。DNA特异性结合到QIAamp MinElute硅胶膜上,污染物流出。无需苯酚-氯仿抽提。通过两步高效洗涤可完全去除PCR抑制物,如二价阳离子和蛋白质。然后用水或试剂盒中的缓冲液洗脱结合在离心柱上的纯的DNA。QIAamp DNA Micro技术可从少量样本中纯化得到基因组DNA和线粒体DNA,可即时用于PCR和印迹实验。

QIAamp样本制备技术完全通过许可认证,其制备的核酸适用于任何分子实验或其他下游应用,而无侵害专利的风险。

Procedure
QIAamp DNA Micro Kit使用快速离心柱流程,简化了从少量样本中纯化DNA的步骤(参见"Procedure")。QIAamp DNA Micro Kit使用成熟的技术,从微量样本(如:1-100 µl全血、~3 mm直径的血卡、尿液、<10 mg的组织或激光显微切割组织)中纯化基因组DNA和线粒体DNA。可在30分钟内纯化新鲜或冷冻的血液、组织和干血斑,洗脱体积为20–100 µl。
Applications

QIAamp DNA Micro Kit适用于多种样本,包括:

  • 痕量血液
  • 血卡
  • 尿液
  • 少量组织样本,包括激光显微切割样本

Specifications

Features
Specifications
应用 Real-time PCR, STR analysis, LMD-PCR
洗脱体积 20–100 µl
规格 Spin column
主要样本类型 Whole blood
处理 Manual (centrifugation or vacuum)
纯化总RNA、miRNA、poly A+ mRNA、DNA或蛋白 Genomic DNA, mitochondrial DNA
样本量 1–100 µl
技术 Silica technology
每次运行或制备时间 30 minutes
产量 <3 µg

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试剂盒操作手册 (1)
For purification of genomic DNA from small volumes of blood, dried blood spots, swabs, forensic case work samples, chewing gum, urine, tissues, laser-microdissected tissues; For cleanup of genomic DNA
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