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RNeasy Plus Mini Kit

For purification of up to 100 µg total RNA from cells/tissues using gDNA Eliminator columns

  • Unique gDNA Eliminator columns avoid the need for DNase
  • Efficient removal of genomic DNA
  • Highly reproducible yields of RNA in minutes
  • High-performance RNA for sensitive applications

The RNeasy Plus Mini Kit integrates fast, convenient purification of up to 100 µg RNA with effective elimination of genomic DNA. Cell or tissue lysates are spun through gDNA Eliminator spin columns to remove genomic DNA. Total RNA is then purified using RNeasy Mini spin columns. The kit can be automated on the QIAcube Connect. Tissue samples can be conveniently stabilized using RNAprotect Tissue Reagent or Allprotect Tissue Reagent, and efficiently disrupted using a TissueRuptor or TissueLyser system. For smaller samples, the RNeasy Plus Micro Kit (spin-column binding capacity of 45 µg RNA) is also available. Request a quote for a trial kit.

Specifications

Features
Specifications
Applications Northern, dot, and slot blotting, end-point RT-PCR, quantitative, real-time RT-PCR, array analysis, next-generation sequencing
Elution volume 30-50 µl
Format Spin column
Integrated removal of genomic DNA Yes
Main sample type Tissue, cells
Processing Manual (centrifugation)
Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein Total RNA
Sample amount 30 mg (<700 µl)
Technology Silica technology
Time per run or per prep 25 minutes
Yield Varies

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Supplementary Protocols (2)

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There are two protocols: follow Protocol 1 if you want to purify total RNA containing miRNA, or follow Protocol 2 if you want to purify small RNA (includes miRNA, 5S rRNA, and tRNA) and larger RNA (>200 nt) separately.
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Kit Handbooks (2)
For purification of total RNA from animal cells and easy-to-lyse animal tissues using gDNA Eliminator columns
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Explore our new, easy-to-navigate digital version of the RNeasy Plus Mini and Micro Kits Product Profile!
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