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RNeasy Plus Micro Kit

For purification of up to 45 µg total RNA from cells/tissues using gDNA Eliminator columns

  • Unique gDNA Eliminator columns avoid the need for DNase
  • Efficient removal of genomic DNA
  • Highly reproducible yields of RNA in minutes
  • High-performance RNA for sensitive applications

The RNeasy Plus Micro Kit integrates fast, convenient purification of up to 45 µg RNA with effective elimination of genomic DNA. Cell or tissue lysates are spun through gDNA Eliminator spin columns to remove genomic DNA. Total RNA is then purified using RNeasy MinElute spin columns. Tissue samples can be conveniently stabilized using RNAprotect Tissue Reagent or Allprotect Tissue Reagent, and efficiently disrupted using a TissueRuptor or TissueLyser system. For larger samples, the RNeasy Plus Mini Kit (spin-column binding capacity of 100 µg RNA) is also available. Procedures can be fully automated on the QIAcube Connect.

Specifications

Features
Specifications
Applications Northern, dot, and slot blotting, end-point RT-PCR, quantitative, real-time RT-PCR, array analysis, next-generation sequencing
Elution volume 14 µl
Format Spin column
Integrated removal of genomic DNA Yes
Main sample type Tissue, cells
Processing Manual (centrifugation)
Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein Total RNA
Sample amount 5 x 10e5 cells or 5 mg tissue
Technology Silica technology
Time per run or per prep 25 min
Yield Varies

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