Rotor-Disc 100 Starter Kit
For use with the Rotor-Gene Q
- Accelerated reaction setup and high throughput
- Outstanding performance in real-time PCR with the Rotor-Gene Q
The Rotor-Disc 100 Starter Kit includes all you need for runs of 100 reactions on the Rotor-Gene Q real-time cycler.
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Rotor-Disc 100 Starter Kit (115V)
3 Rotor-Disc 100 packs for 15–25 µl reaction volumes, Rotor-Disc Heat Sealer, Rotor-Disc Heat Sealing Film, Rotor-Disc 100 Rotor and Locking Ring, Rotor-Disc Pipetting Aid, Rotor-Disc Easy Lift Adaptor
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9001602
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Rotor-Disc 100 Starter Kit (230V)
3 Rotor-Disc 100 packs for 15–25 µl reaction volumes, Rotor-Disc Heat Sealer, Rotor-Disc Heat Sealing Film, Rotor-Disc 100 Rotor and Locking Ring, Rotor-Disc Pipetting Aid, Rotor-Disc Easy Lift Adaptor
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9001603
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The Rotor-Disc 100 Starter Kit is for use with the Rotor-Gene Q thermal cycler.
Principle
The Rotor-Disc 100 Starter Kit enables easy start up of real-time PCR experiments on the Rotor-Gene Q using the Rotor-Disc 100 format. The kit includes Rotor-Disc 100 packs, a Rotor-Disc Heat Sealer, Rotor-Disc Heat Sealing Film, a Rotor-Disc 100 Rotor and Locking Ring, a Rotor-Disc 100 Loading Block, and a Rotor-Disc Pipetting Aid.
Rotor-Discs are fully compatible with the QIAgility instrument, which enables automated reaction setup.
Procedure
The Rotor-Disc 100 Rotor and Rotor-Disc 100 Locking Ring are used with the Rotor-Disc 100. The Rotor-Disc 100 is a circular plate of 100 vertically oriented reaction wells for high-throughput use. The Rotor-Disc 100 is the rotary equivalent of a 96-well plate but with an additional 4 reference wells. It enables integration of the Rotor-Gene Q with 96-well laboratory workflows. The extra wells can be conveniently used for more samples, additional control reactions, or orientation reactions, without occupying any of the standard 96-well positions. For seamless 96-well workflow compatibility, Rotor-Disc 100 wells use 96-well plate labeling conventions, i.e., A1-A12 through to H1-H12. The additional 4 reference wells are labeled R1-R4.
The Rotor-Disc 100 does not use caps. Instead, Rotor-Disc Heat Sealing Film is applied to the top and heat sealed using a Rotor-Disc Heat Sealer. The film prevents contamination by providing a strong, durable, and tamper-proof seal.
Applications
The Rotor-Gene Q and Rotor-Disc 100 Starter Kit enable real-time PCR and high-resolution melting (HRM) applications, including:
- Gene expression analysis
- Pathogen detection
- DNA methylation analysis
- Genotyping
- Gene scanning
- miRNA research
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Calibration guide for use with QIAgility and the Rotor-Disc 100
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