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MagAttract Virus Mini M48 Kit

For simultaneous purification of viral DNA and RNA from serum and plasma
  • Higher analytical sensitivity even with low viral titers
  • Robust procedure minimizes risk of cross-contamination
  • Linear yields from a wide range of viral titers
  • Walkaway processing enables efficient workflow

The MagAttract Virus Mini M48 Kit provides automated purification of viral DNA and RNA on the BioRobot M48 using proven MagAttract magnetic particle technology. Viral nucleic acid purification and magnetic separation take place in the pipet tips, increasing the efficiency of the procedure.

Cat No./ID: 955336
MagAttract Virus Mini M48 Kit (192)
S Fr.827.00
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For 192 virus nucleic acid preps: MagAttract Suspension B and RNase-free reagents and buffers
The MagAttract Virus Mini M48 Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
0
High sensitivity for reliable detection.
Serial dilutions from a negative plasma pool spiked with an international standard of a typical DNA virus were processed in replicates of 24, using the MagAttract Virus Mini M48 Kit with a 400 µl input volume (M48/400), or using automated kits from Supplier R with a 1000 µl (R/1000) or 200 µl (R/200) input volume. Samples were analyzed by real-time duplex PCR with an internal control. The vertical lines show the 95% hit rate at which detection by PCR can be expected with a 95% probability.
1
Highly reproducible purification — A.
A negative plasma pool was spiked on each of 3 days with a typical DNA or RNA virus. Viral nucleic acids were purified from 24 aliquots (400 µl each) of each pool using the MagAttract Virus Mini M48 Kit, with elution in a volume of 125 µl. Pools were spiked with 105 copies/ml of a typical DNA virus. Purified viral DNA (10 µl of each eluate) was analyzed by real-time PCR using the QuantiTect SYBR® Green PCR Kit, and CT values are shown for 24 samples on each day.
2
Highly reproducible purification — B.
A negative plasma pool was spiked on each of 3 days with a typical DNA or RNA virus. Viral nucleic acids were purified from 24 aliquots (400 µl each) of each pool using the MagAttract Virus Mini M48 Kit, with elution in a volume of 125 µl. Pools were spiked with 104 copies/ml of a typical RNA virus. Purified viral RNA (10 µl of each eluate) was analyzed by real-time RT-PCR using the QuantiTect Probe RT-PCR Kit, and CT values are shown for 24 samples on each day.
Performance

The MagAttract Virus Mini M48 Kit can be used to purify nucleic acids from a broad range of DNA and RNA viruses. The yields of viral nucleic acids obtained in the purification procedure are normally below 1 µg and therefore quantitative amplification methods should be used to determine yields. The automated procedure gives more sensitive detection for the amount of starting material than other automated systems that use magnetic beads (see figure "High sensitivity for reliable detection"). Fully automated processing on the BioRobot M48 instrument provides highly reproducible purification, with minimal sample-to-sample or day-to-day variation (see figures "Highly reproducible purification — A and  B"). Parallel purification of 48 negative and positive (107 copies/ml armored RNA) samples with the MagAttract Virus Mini M48 Kit on the BioRobot M48 results in no detectable cross-contamination (see table). Analysis was by real-time RT-PCR.

No detectable cross-contamination.
RunNumber of positive samples* Number of negative samples†

Run 1
Positive detection 24 0
Negative detection 0 24
Run 2
Positive detection 24 0
Negative detection 0 24
* The mean CT value for positive samples was 29.9.
† Samples with CT >60 were regarded as negative.

Principle

The MagAttract Virus Mini M48 Kit enables highly sensitive detection of a broad range of DNA and RNA viruses. Yields are linear, allowing accurate quantitative analysis for both low and high viral titers. Accurate detection requires cross-contamination-free processing to avoid false positives. The MagAttract Virus Mini M48 Kit on the BioRobot M48 processes samples with no detectable cross-contamination and is well-suited for purification of both high and low levels of viral nucleic acids from the same set of samples. Kit performance is not guaranteed for each virus species and has to be validated by the user.

Procedure

Fully automated processing with the MagAttract Virus Mini M48 Kit on the BioRobot M48 provides highly reproducible purification in a procedure designed to ensure safe handling of potentally infectious samples. The purification procedure comprises 4 steps: lyse, bind, wash, and elute. MagAttract Virus Mini M48 Kit protocols allow different options for addition of internal control either at the lysis step or at the binding step, depending on the lysis requirement of packaged or native internal controls. Following the washing step, highly pure viral DNA and RNA are eluted in buffer equilibrated to room temperature. Walkaway purification of 48 samples requires only 15–20 minutes setup time, allowing quick turnaround and flexible processing.

Applications

The MagAttract Virus Mini M48 Kit provides a fully automated procedure for simultaneous purification of viral DNA and RNA from serum and plasma for highly sensitive detection in downstream assays. The kit can be used to purify nucleic acids that are free of proteins, nucleases, and other impurities from a broad range of DNA and RNA viruses for life science applications.

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