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QIAamp Virus BioRobot MDx Kit

For automated purification of viral DNA and RNA from cell-free body fluids on the BioRobot MDx workstation
  • Rapid isolation of high-quality, ready-to-use viral DNA and RNA
  • No organic extraction or alcohol precipitation
  • Consistent, high yields
  • Removal of contaminants and inhibitors

The QIAamp Virus BioRobot MDx Kit provides automated viral nucleic acid purification on the BioRobot MDx using proven QIAamp technology. The fully automated procedure requires less than 2.5 hours, including bar code reading and complete process documentation, with no hands-on time during the run.

Cat No./ID: 965652
QIAamp Virus BioRobot MDx Kit (12)
S Fr.6,435.00
Add To Cart
For 12 x 96 preps for use on the BioRobot MDx; includes: 12 QIAamp 96 Plates, RNase-free buffers, QIAGEN Protease, Elution Microtubes CL, caps, S-Blocks, carrier RNA
The QIAamp Virus BioRobot MDx Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.

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QIAamp Virus BioRobot MDx protocol.
Performance

The QIAamp Virus BioRobot MDx process provides uniform DNA and RNA recovery and purity across the 96-well plate with no detectable cross-contamination between samples. Up to 265 µl of cell-free body fluids can be processed. The typical elution volume is 75 µl. Viruses can be detected with high sensitivity when using this kit, which is illustrated by hit-rate studies (see table). Further advantages of the system include high process reliability and walkaway automation, providing a highly efficient system for purification of viral nucleic acids. The QIAamp Virus BioRobot MDx provides reliable results in downstream applications.

High precision using the QIAamp Virus BioRobot MDx Kit
PCR product (n=96) Instrument 1 Instrument 2
Mean CT Standard deviation %CV Mean CT Standard deviation %CV
Mimic virus 28.26 0.10 0.35 28.38 0.18 0.65
Internal control 31.98 0.16 0.51 31.87 0.22 0.70
Plasma was spiked with armored RNA molecules to mimic RNA viruses (25,000 copies per prep) and processed using the QIAamp Virus BioRobot MDx protocol in replicates of 96 on two separate instruments. An internal control RNA was added to the lysis buffer. The eluted RNA was amplified using a TaqMan® RT-PCR system. Mean CT value, standard deviation, and %CV were calculated for both the mimic virus and the internal control. (Data generated by QIAGEN R&D department.)
Principle

The QIAamp Virus BioRobot MDx Kit simplifies isolation of viral RNA and DNA from cell-free body fluids with a fast automated 96-well–plate procedure. No phenol–chloroform extraction is required. Nucleic acids bind specifically to the QIAamp silica-gel membrane while contaminants pass through. PCR inhibitors, such as divalent cations and proteins, are completely removed in two efficient wash steps, leaving pure nucleic acids to be eluted in either water or a buffer provided with the kit. QIAamp technology yields viral RNA and DNA from cell-free body fluids ready to use in PCR and blotting procedures.

The fully automated procedure, including bar code reading and complete process documentation, requires less than 2.5 hours to process 96 samples (including a detailed load check of 20 minutes) with no hands-on time during the run. Advantages of the system include high process reliability and walkaway automation, providing a highly efficient system for purification of viral nucleic acids for laboratories. QIAamp sample preparation technology is fully licensed.

Procedure
Optimized buffers lyse samples, stabilize nucleic acids, and enhance selective nucleic acid adsorption to the QIAamp membrane. Alcohol is added and lysates loaded onto the QIAamp 96-well plate. Wash buffers are used to remove impurities and pure, ready-to-use nucleic acids are then eluted in water or low-salt buffer (see flowchart "Protocol").
Applications

The QIAamp Virus BioRobot MDx Kit provides automated, high-throughput purification of viral RNA and DNA on the BioRobot MDx using proven QIAamp technology. The 96-well plates provide the flexibility of processing 32–96 samples, in increments of 8. Sample sources include:

Plasma and serum
CSF
Other cell-free body fluids
Cell-culture supernatants
Features
Specifications
Applications PCR, qPCR, real-time PCR
Elution volume 75 µl
For automated processing BioRobot MDx Workstation
Format 96-well plates
Main sample type Serum, plasma
Processing Automated
Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein Viral DNA, viral RNA
Sample amount 265 µl
Samples per run; throughput 36-92 samples per run
Technology Silica technology
Time per run or per prep <2.5 hours
Yield >90% recovery
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