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MagAttract DNA Mini M48 Kit

For automated purification of genomic DNA from 200 µl lysates of a wide range of human samples
  • High-quality DNA from a wide range of sample types
  • Flexible purification of 6–48 samples per run

The MagAttract DNA Mini M48 Kit provides automated purification of genomic DNA on the BioRobot M48 workstation using proven MagAttract magnetic particle technology. DNA purification and magnetic separation take place in the pipet tips, increasing the efficiency of the procedure. Up to 48 samples can be processed per run in increments of 6 samples. A wide range of sample material can be processed including tissue, cells, buccal swabs, dried blood, forensic and human-identity samples, paraffin-embedded tissues, and bacteria.

Cat No./ID: 953336
MagAttract DNA Mini M48 Kit (192)
S Fr.938.00
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For 192 DNA preps: MagAttract Suspension B, Buffers, Proteinase K
The MagAttract DNA Mini M48 Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
Performance

DNA yields depend on the sample type, number of nucleated cells in the sample, and the protocol used for isolation of DNA.

Principle

MagAttract DNA M48 Kits are designed for fully automated DNA purification. Proven MagAttract magnetic particle technology in combination with the BioRobot M48 workstation provides high-quality DNA, suitable for direct use in sensitive downstream applications. The purified DNA is free of PCR inhibitors. Advantages of the system include scalable sample volumes and elution volumes, providing a highly efficient and cost-effective DNA purification system.

Procedure

MagAttract technology combines the speed and efficiency of silica-based DNA purification with the convenient handling of magnetic particles. Optimized buffers lyse samples, and DNA binds to the silica surface of the magnetic particles in the presence of a chaotropic salt. DNA bound to the magnetic particles is then efficiently washed. Two different wash buffers are used, followed by a rapid rinse with distilled water, which considerably improves the purity of the DNA. High-quality DNA is eluted in the water provided. The sample and elution volumes for each protocol are scalable, giving the yield and concentration of high-quality DNA appropriate for the intended downstream application.

Applications

The high-quality DNA obtained using the MagAttract DNA Mini M48 Kit and the BioRobot M48 workstation is suited for use in many downstream applications, such as:

Genotyping analysis (e.g., STR, SNP, VNTR, RAPD, NASBA, and AFLP)
Infectious disease and oncology research
Purification of DNA from archived samples
Population genetics
Features
Specifications
Applications Genotyping, oncology research, population genetics
Elution volume 50–400 µl
For automated processing BioRobot M48 Workstation
Format Sample tube (1.5 ml, 2 ml)
Main sample type Tissue, cells, blood
Processing Automated
Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein Genomic DNA
Sample amount 200 µl/10– 40 mg
Technology Magnetic particles
Time per run or per prep 23 minutes – 2.5 hours
Yield 9–28 µg

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Kit Handbooks (2)
MagAttract DNA Mini M48 Kit For DNA purification from soft tissues and cultured cells using the BioRobot M48 workstation
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For automated purification of DNA from forensic and biosecurity samples using the BioRobot M48 workstation  
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Quick-Start Protocols (2)
For purification of DNA from soft tissues and mouse tail
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For purification of DNA from cultured cells
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