BioSprint 15 DNA Plant Kit
For rapid and economical automated purification of total DNA from plant tissue
The BioSprint 15 DNA Plant Kit provides automated purification of total DNA from plant tissue on the BioSprint 15 using proven MagAttract magnetic particle technology. The kit contains all reagents and plasticware necessary for automated DNA purification.
The BioSprint 15 DNA Plant Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
Performance
DNA can be successfully purified from a variety of plant species and material (see table).
Purified DNA is up to 40 kb in size. The DNA is ready to use in a range of downstream applications, including PCR and real-time PCR, genotyping, and microsatellite analysis (see figure "High PCR performance of DNA purified using the BioSprint 15 DNA Plant Kit").
Principle
The BioSprint 15 DNA Plant Kit is used with the BioSprint 15. The kit uses magnetic-particle technology for rapid DNA purification from plant material. The BioSprint 15 controls an array of magnetic rods that can attract or release magnetic particles and transfer them from tube to tube. The sequential transfer of magnetic particles allows a rapid purification procedure to be performed, from the initial binding of DNA to the particles, through to washing of the particles and elution of pure DNA (see figure "Efficient processing of magnetic particles"). Since the instrument transfers magnetic particles instead of liquids, it uses minimal amounts of reagents, enabling cost-efficient sample preparation.
Procedure
The BioSprint 15 DNA Plant Kit enables purification of total DNA from a wide variety of plant species, including difficult plant material. Fresh, frozen, or lyophilized starting material is mechanically disrupted to give a fine powder using a homogenizer such as the TissueLyser LT or TissueLyser II. The powder is resuspended in lysis buffer, thoroughly mixed, and then sedimented by a short centrifugation step. Cleared plant lysates are transferred to a 5-well strip and loaded into the instrument, which then performs all steps until pure DNA is obtained (see flowchart "BioSprint DNA Plant procedure"). The kit provides all the necessary reagents and plasticware for sample preparation, and is available in different sizes, allowing cost-efficient use of consumables.
Applications
The BioSprint 15 DNA Plant Kit purifies DNA for use in a range of downstream applications, including:
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