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QIAamp DNA Blood BioRobot 9604 Kit

For automated purification of genomic and mitochondrial DNA from whole blood using the BioRobot 9604 workstation
  • Rapid isolation of high-quality, ready-to-use DNA
  • No organic extraction or alcohol precipitation
  • Consistent, high yields
  • Removal of contaminants and inhibitors

The QIAamp DNA Blood BioRobot 9604 Kit provides automated DNA purification on the BioRobot 9604 workstation using proven QIAamp silica-membrane technology. The fully automated procedure on the BioRobot 9604 workstation requires less than 2.5 hours, including bar code reading and complete process documentation, with no hands-on time during the run.

Cat No./ID: 965162
QIAamp DNA Blood BioRobot 9604 Kit (12)
S Fr.4,444.00
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For 12 x 96 DNA preps: 12 QIAamp 96 Plates, Buffers, QIAGEN Protease, AirPore Tape Sheets, Tape Pad, S-Blocks, Racks with Collection Microtubes (1.2 ml), Caps
The QIAamp DNA Blood BioRobot 9604 Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
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QIAamp 96 DNA Blood procedure.
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Cross-contamination-free purification for reliable PCR.

Cross-contamination control analysis of the automated DNA purification procedure on the BioRobot 9604. Samples (200 µl) of blood or water were added alternately to wells in the QIAamp 96 plate and the entire 96-well plate was processed following the QIAamp 96 DNA Blood BioRobot procedure. Five microliters of each 200 µl eluate was submitted to amplification in a 50 µl PCR using HotStarTaq DNA Polymerase, and the resultant products were analyzed on an agarose gel. No products were detected from eluates corresponding to wells containing water samples. Markers: Low DNA Mass Ladder (Gibco BRL).

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Reproducible DNA yield.
Yield (measured in water) of 20 typical DNA preps from 96 preps purified from a single sample of human whole blood using the QIAamp 96 DNA Blood BioRobot procedure. Mean values and standard errors represent all 96 preps from the QIAamp 96 plate.
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Reproducible DNA purity.
Purity (measured in water) of 20 typical DNA preps from 96 preps purified from a single sample of human whole blood using the QIAamp 96 DNA Blood BioRobot procedure. Mean values and standard errors represent all 96 preps from the QIAamp 96 plate.
Performance

QIAamp 96-well plates provide well-to-well uniformity in DNA recovery and purity (see figures "Reproducible DNA yield" and "Reproducible DNA purity"), and PCR experiments show no cross-contamination between samples in adjacent wells (see figure "Cross-contamination-free purification for reliable PCR"). As many as 96 samples of human whole blood or other body fluids can be processed yielding purified DNA in under 2 hours with minimal hands-on time. QIAamp sample preparation technology is fully licensed.

The QIAamp 96 DNA Blood procedure on the BioRobot 9604 increases productivity and reliability. DNA purified using the QIAamp 96 DNA Blood procedure on the BioRobot 9604 is free of contaminants and enzyme inhibitors, and is ready for use in amplification, Southern blotting, or other enzymatic assays for

Genetic disease testing
Identity testing
Cancer screening
Principle

No phenol–chloroform extraction is required. DNA binds specifically to the QIAamp silica-gel membrane while contaminants pass through. PCR inhibitors, such as divalent cations and proteins, are completely removed in three efficient wash steps, leaving pure DNA to be eluted in either water or a buffer provided with the kit. QIAamp technology yields genomic and mitochondrial DNA from blood and other body fluids ready to use in PCR and blotting procedures.

Fresh and frozen whole blood with common anticoagulants, such as citrate, EDTA, and heparin, may be processed with additional equipment and the use of a special protocol available from QIAGEN Technical Services or your local distributor.

Procedure
The QIAamp DNA Blood BioRobot 9604 Kit simplifies isolation of DNA from blood and other body fluids with a fast automated 96-well–plate procedure. Using the BioRobot 9604 Workstation, the QIAamp DNA Blood BioRobot 9604 Kit processes up to 200 µl sample size, with preparation time of 96 samples in less than 2 hours (see "Protocol"). The automated procedure, including bar code reading and process documentation, needs about 5 minutes hands-on time, with a turnaround time between consecutive runs of about 10 minutes.  The typical yield is 3–6 µg per 200 µl healthy whole blood, with an elution volume of 200 µl.
Applications

The QIAamp DNA Blood BioRobot 9604 Kit provides automated high-throughput DNA purification on the BioRobot 9604 using proven QIAamp technology. The kit processes 96-well plates for up to 96 preps. Sample types include:

Fresh or frozen whole blood
Serum
Bone marrow
Body fluids
Cell suspensions
Features
Specifications
Applications PCR, blotting
Elution volume 200 µl
For automated processing BioRobot 9604
Format 96-well plate
Main sample type Whole blood
Processing Automated
Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein Genomic DNA, mitochondrial DNA
Sample amount 200 µl
Technology Silica technology
Time per run or per prep <2 hours
Yield 3–6 µg

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Kit Handbooks (1)
For purification of genomic DNA from whole blood buffy coat bone marrow body fluids lymphocytes cultured cells using the BioRobot 9604 workstation
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