text.skipToContent text.skipToNavigation

PAXgene Blood DNA Kit

For purification of up to 500 µg genomic DNA from whole blood
  • Blood collection and purification in one system
  • Easy handling
  • Enhanced workflow efficiency

The PAXgene Blood DNA system consists of PAXgene Blood DNA Tubes, for blood collection and stabilization, and the PAXgene Blood DNA Kit, for DNA purification in a single-tube procedure.

Cat No./ID: 761133
PAXgene Blood DNA Kit (25)
S Fr.513.00
Add To Cart
Processing tubes and buffers for 25 preparations
For Research Use Only. Not for use in diagnostics procedures. No claim or representation is intended to provide information for the diagnosis, prevention, or treatment of a disease.
0
PAXgene Blood DNA procedure.
Blood samples (8.5 ml) are collected in PAXgene Blood DNA Tubes, where they may be stored or transported. For DNA isolation, the blood is transferred to processing tubes (supplied already filled with cell lysis buffer), and the solution is mixed to lyse red and white blood cells. Cell nuclei and mitochondria are pelleted by centrifugation, washed, and resuspended in digestion buffer. Protein contaminants are removed by incubation with a protease. DNA is precipitated in isopropanol, washed in 70% ethanol, dried, and resuspended in resuspension buffer.
1
Efficient multiplex PCR of 3 mitochondrial genes.
Multiplex PCR of fragments from the mitochondrial genes tRNAlys/ATPase (0.92 kb), tRNAleu(UUR) (0.63 kb), and ND4 (0.29 kb), using 250 ng DNA from 8 donors as starting material.
2
High quality and high molecular weight of genomic DNA.
Genomic DNA isolated from 8 blood donors using the PAXgene Blood DNA System. [A] Agarose gel analysis; [B] pulsed-field gel electrophoresis for enhanced separation of high-molecular-weight genomic DNA.  M: markers.
Performance
DNA isolated using the PAXgene Blood DNA System has an A260/A280 ratio of 1.7–1.9. Fragments are 20–200 kb in size, with an average length of 50–150 kb (see figure "High quality and high molecular weight of genomic DNA"). Average DNA yields are 150–500 µg, depending on the number of nucleated cells present in the blood sample. Purified DNA can be used in PCR and quantitative, real-time PCR (see figure "Efficient multiplex PCR of 3 mitochondrial genes").
Principle

The PAXgene Blood DNA System is an integrated and standardized system for collection and stabilization of whole blood specimens and isolation of their genomic DNA. The system requires the combined use of PAXgene Blood DNA Tubes for blood collection and stabilization, followed by DNA isolation using the PAXgene Blood DNA Kit.

Blood is collected into PAXgene Blood DNA Tubes, which contain a proprietary blend of reagents that both prevents blood coagulation and stabilizes white blood cells. Blood samples can be stored in the tubes for up to 14 days at room temperature. DNA is isolated from the tubes using appropriate buffers supplied in the PAXgene Blood DNA Kit. Highly pure genomic DNA is obtained, suitable for use in a wide range of downstream applications.

Procedure

The PAXgene Blood DNA procedure is shown in the flowchart "PAXgene Blood DNA procedure". Blood samples (8.5 ml) are collected in PAXgene Blood DNA Tubes, where they may be stored or transported. For DNA isolation, the blood is transferred to processing tubes (supplied already filled with cell lysis buffer), and the solution is mixed to lyse red and white blood cells. Cell nuclei and mitochondria are pelleted by centrifugation, washed, and resuspended in digestion buffer. Protein contaminants are removed by incubation with a protease. DNA is precipitated in isopropanol, washed in 70% ethanol, dried, and resuspended in resuspension buffer.

Applications

The DNA can be used in a wide range of downstream applications, including:

  • PCR and quantitative, real-time PCR 
  • Southern blotting
  • SNP genotyping
  • Pharmacogenomic studies
  • SNP discovery and Validation
Features
Specifications
Applications PCR, qPCR, real-time PCR, blotting, genotyping
Elution volume 1 ml
Format Processing tube (salting out method)
Main sample type Whole blood
Processing Manual (centrifugation)
Sample amount 8.5 ml
Time per run null
Yield 150–500 µg

You are not authorized to download the resource

Brochures & Guides (3)
Second edition — innovative tools
Show details

Show details
For collection, storage, and transport of whole blood for genomic DNA isolation

 


Show details
FAQs (18)
FAQ ID -12
View
FAQ ID - 3497
View
FAQ ID - 3498
View
FAQ ID - 3499
View
FAQ ID - 3500
View
FAQ ID - 3501
View
FAQ ID - 3502
View
FAQ ID - 3503
View
FAQ ID - 3504
View
FAQ ID - 3505
View
FAQ ID - 3507
View
FAQ ID - 3508
View
FAQ ID - 3509

View
FAQ ID - 3510
View
FAQ ID - 3511

View
FAQ ID - 3512

View
FAQ ID - 3515
View
FAQ ID - 3506
View
Kit Handbooks (1)
For isolation of genomic DNA from 8.5 ml human whole blood
Show details
fragment fix placeholder

Customers who bought these products also bought