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DNeasy Plant Maxi Kit

For isolation of up to 260 µg total cellular DNA from plant cells and tissues or fungi

  • Pure DNA, free from contaminants and enzyme inhibitors
  • Rapid isolation of ready-to-use DNA
  • No organic extraction, no ethanol precipitation

The DNeasy Plant Maxi Kit provides fast and easy silica-based DNA purification in spin column format. Typical yields are 30–260 μg of high-quality DNA, depending on the sample source.

Cat No./ID: 68163
DNeasy Plant Maxi Kit (24)
S Fr.391.00
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24 DNeasy Maxi Spin Columns, 24 QIAshredder Maxi Spin Columns, RNase A, Buffers, Collection Tubes (50 ml)
The DNeasy Plant Maxi Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
1
PCR performance.
DNA was isolated from arabidopsis leaves using either CTAB lysis (CTAB) or the DNeasy Plant Maxi Kit (DNeasy). Amplification reactions were prepared using purified DNA (1: 50 pg; 2: 100 pg) and primers to the Akin 10 gene. M: markers. (Data kindly provided by Alain Lecharny, Institut de Biotechnologie des Plantes, UMR CNRS-UPS Orsay, France.)
2
PCR analysis.
DNA (10 ng) from the indicated leaves or needles was amplified using universal primers for the noncoding intergenic spacer between the tRNA genes trnL (UAA) 5' exon and trnL (UAA) 3' exon of cpDNA (Taberlet, P. et al. (1991) Plant Mol. Biol. 17, 1105). M: 100 bp ladder.
3
RAPD analysis.
DNA (50 ng) from leaves of the indicated in vitro-propagated sunflower (Helianthus) species was amplified using a 10-base RAPD primer and separated on a 1.5% agarose gel. M: 100 bp ladder. (Data kindly provided by H. J. Henn, Institute of Agricultural Botany, University of Bonn, Germany.)
Performance

The DNeasy Plant Maxi Kit allows rapid and efficient isolation of high-quality DNA from a wide variety of plant species and tissue types including the most demanding sources (see table "Selection of plant species processed with DNeasy Kits"). Samples may be fresh, frozen, or dried. The optimized DNeasy Plant procedure incorporates the QIAshredder Maxi spin column, a unique filtration and homogenization column that efficiently removes cell debris and improves sample handling following lysis.

Selection of plant species processed with DNeasy Kits

Abies alba (silver fir) Nicotiana tabacum (tobacco)
Aesculus hippocastanum (horse chestnut) Oryza sativa (rice)4
Arabidopsis thaliana (thale cress) Pelargonium sp. (geranium)4
Avena sp. (oat) Petunia sp.4
Brassica napus (oilseed rape) Pinus sylvestris (Scotch pine), P. brutia5
Brassica oleracea (kohlrabi) Populus tremula (aspen)
Chicorium endivia (chicory) Pseudotsuga menziesii (Douglas fir)
Citrullini lanatus (water melon) Quercus robur, Q. petrea (oak)6,7
Egeria sp. Rhododendron sp.2,4
Fagus sylvatica (beech)1 Rubus idaeus (raspberry)
Helianthus spp. (sunflower) Solanum tuberosum (potato)
Hordeum vulgare (barley)2 Sphagnum palustre (moss)
Humulus sp. (hops) Spinacia oleracea (spinach)
Hydrilla sp. Taxus baccata (yew)
Kalanchoe spp. Triticum aestivum (wheat)4
Lupinus sp. Ulmus glabra (elm)6
Lycopersicon esculentum (tomato)3 Vitis spp. (grape)6
Myriophyllum sp. Zea mays (maize)

Young leaves or needles (and other tissues, as indicated) were collected and immediately flash frozen. DNA isolation was then performed with the DNeasy Plant Mini Kit. 1Beechnut, 2dried leaves, 3callus, 4leaves from adult plant, 5endosperm, 6old leaves, rich in carbohydrates, 7buds. For more information on DNA isolation from other species including fungi, call QIAGEN Technical Services or your local distributor.

The typical yield is 30–260 µg, with a sample size of up to 1 g wet weight, and an elution volume of 500 µl to 2 ml. The DNA yields vary between different species and tissues depending on genome size, ploidy, cell number, and age of tissue sample. Lower and higher range values correspond to arabidopsis and wheat, respectively. Absorbance scans of DNeasy purified DNA show a symmetrical peak at 260 nm (see figures "DNA purity from oak leaves and pine needles"), confirming that the DNA is free of impurities, including enzyme inhibitors. Purified DNA can be used in a wide range of applications (see figures "PCR performance", "PCR analysis", and "RAPD analysis").

Principle

DNeasy Plant Kits use advanced silica-membrane technology and simple spin procedures to isolate highly pure total cellular DNA from plant tissues and cells or fungi. DNeasy technology replaces cumbersome DNA isolation procedures such as cetyltrimethylammonium bromide (CTAB), phenol, or chloroform extraction. Using the DNeasy procedure, alcohol precipitation is not necessary — purified DNA is ready for immediate use. DNeasy sample preparation technology is fully licensed.

Procedure

Samples are first mechanically disrupted and then chemically lysed (see flowchart "DNeasy Plant and DNeasy 96 Plant procedures"). RNA is removed by RNAse digestion during lysis. Cell debries, precipitated proteins, and polysaccharides are removed and the sample is homogenized by centrifugation through a QIAshredder Maxi spin column. Buffering conditions are adjusted and the lysate is loaded onto the DNeasy Plant Maxi spin column. During a brief spin, DNA selectively binds to the silica membrane while contaminants pass through. Remaining contaminants and enzyme inhibitors are removed in one or two efficient wash steps. Pure DNA is then eluted in water or low-salt buffer, ready for use.

Applications

The DNeasy Plant Maxi Kit provides purification of DNA from plant tissue, including:

Plant cells
Plant tissues
Fungi
Features
Specifications
Applications PCR, real-time PCR, blotting
Elution volume 500 µl – 2 ml
Format Spin column
Main sample type Plant samples
Processing Manual
Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein DNA
Sample amount <1 g
Technology Silica technology
Time per run or per prep <2 hours
Yield 30–260 µg
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