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QIAamp MinElute Virus Spin Kit

For simultaneous purification of viral DNA and RNA from plasma, serum, and cell-free body fluids

  • Rapid purification of high-quality viral DNA and RNA
  • No organic extraction or alcohol precipitation
  • Consistent, high yields
  • Complete removal of contaminants and inhibitors

The QIAamp MinElute Virus Spin Kit simplifies purification of viral DNA and RNA with fast spin-column procedures. The QIAamp MinElute Virus Spin Kit uses starting sample volumes of up to 0.2 ml and combines the selective binding properties of a silica-based membrane with flexible elution volumes of between 20 and 150 μl. The QIAamp MinElute Virus Spin process can be fully automated on the QIAcube.

Cat No./ID: 57704
QIAamp MinElute Virus Spin Kit (50)
S Fr.342.00
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For 50 minipreps: 50 QIAamp MinElute Columns, QIAGEN Protease, carrier RNA, buffers, Collection Tubes (2 ml)
The QIAamp MinElute Virus Spin Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
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QIAamp MinElute Virus Spin procedure.
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High sensitivity in RT-PCR.
The percentage of positive samples at low virus titers is shown. Nucleic acids from an RNA virus were purified from plasma using either the QIAamp MinElute Spin or Vacuum Kit and subject to RT-PCR. For the spin procedure, 95% probit values for 32 samples were 22.84 IU/ml and for the vacuum procedure, they were 9.46 IU/ml.
Performance

Purified viral nucleic acids are free of proteins, nucleases, and other impurities, and suitable for use in sensitive downstream applications such as PCR and RT-PCR (see figures "High sensitivity in PCR" and "High sensitivity in RT-PCR").

Viral nucleic acids purified using the QIAamp MinElute Virus Spin Kit can be used in a wide range of downstream applications, including:

PCR and quantitative real-time PCR
Infectious disease research
Principle

The QIAamp MinElute Virus Spin Kit simplifies isolation of viral RNA and DNA from plasma, serum, and cell-free body fluids with a fast spin-column procedure. No phenol–chloroform extraction is required. Nucleic acids bind specifically to the QIAamp MinElute silica-gel membrane while contaminants pass through. PCR inhibitors, such as divalent cations and proteins, are completely removed in two efficient wash steps, leaving pure nucleic acids to be eluted in either water or a buffer provided with the kit. QIAamp MinElute technology yields viral RNA and DNA from plasma, serum, and cell-free body fluids ready to use in PCR and blotting procedures.

The QIAamp MinElute Spin Kit combines the selective binding properties of a silica-based membrane with flexible elution volumes of between 20 and 150 µl. The procedure is suitable for use with plasma, serum, and other cell-free body fluids. QIAamp sample preparation technology is fully licensed.

Procedure
Optimized buffers lyse samples, stabilize nucleic acids, and enhance selective DNA adsorption to the QIAamp MinElute membrane (see flowchart "QIAamp MinElute Virus Spin procedure"). Alcohol is added and lysates loaded onto the QIAamp MinElute spin column. Wash buffers are used to remove impurities and viral nucleic acids are eluted in Buffer AVE, ready for use in amplification reactions or storage at –20ºC. Purified nucleic acids are free of proteins, nucleases, and other impurities.
Applications

The QIAamp MinElute Virus Spin Kit uses well-established technology for simultaneous purification of viral RNA and DNA from:

Fresh or frozen plasma
Serum
Other cell-free body fluids
Features
Specifications
Applications PCR, real-time PCR
Elution volume 20–150 µl
Format MinElute columns
Main sample type Serum, plasma
Processing Manual (centrifugation)
Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein Viral DNA, viral RNA
Sample amount 200 µl
Technology Silica technology
Time per run or per prep <1 hour
Yield Varies

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