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miRCURY LNA RT Kit

For optimal first-strand cDNA synthesis with the miRCURY LNA miRNA PCR System
  • Optimized for use with miRCURY LNA miRNA PCR Assays and Panels
  • Fast, easy and reliable first-strand synthesis
  • Includes RNA spike-in template for monitoring performance

The miRCURY LNA RT Kit enables fast and convenient miRNA polyadenylation and reverse transcription in a single reaction step and has been designed for use with the miRCURY LNA miRNA PCR System. Using one universal RT reaction as the template for subsequent PCR amplifications greatly simplifies the procedure compared with systems that require miRNA-specific first-strand synthesis, adding convenience and saving you time.

The former Exiqon "miRCURY LNA Universal RT microRNA PCR System" has been updated to the "miRCURY LNA miRNA PCR System" with new reagents that ensure the same superior performance:

  • The Universal cDNA Synthesis Kit II has been replaced by the miRCURY LNA RT Kit
  • The ExiLENT SYBR Green Master Mix Kit has been replaced by the miRCURY LNA SYBR Green PCR Kit
  • Most miRCURY LNA miRNA PCR Assays remain unchanged, but 15 validated assays have been redesigned; refer to the Product Resources tab for this assay list
  • The ready-to-use PCR panels, including the redesigned assays, have been updated to v5 PCR panels

The Exiqon v4 miRCURY LNA Universal RT microRNA PCR System reagents will continue to be available in limited quantities during a transition period. To order, please contact our Support Team directly.

Cat No./ID: 339340
miRCURY LNA RT Kit
S Fr.378.00
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For 8–64 cDNA synthesis reactions: 5x RT Reaction Buffer, 10x RT Enzyme Mix, UniSp6, RNA Spike-in template, RNase-Free Water
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Accurate quantitation from down to 1 pg total RNA starting material.
Data from serial dilutions of human AM6000 total RNA are shown. All miRNA assays exhibit linear readout with correlation coefficients R2 > 0.99.
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miRCURY LNA miRNA PCR System at a glance.
The miRCURY LNA miRNA PCR System uses one single cDNA synthesis reaction for all amplifications, reducing pipetting and saving time and sample. Two LNA-enhanced miRNA-specific qPCR primers enable highly specific and sensitive amplification and single nucleotide discrimination. The fast and easy workflow takes only 3 hours with minimal hands-on steps.


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The benefit of a universal reverse transcription step.
One universal RT reaction per RNA sample saves not only reagents and precious samples, but also your time.
Performance
Unmatched sensitivity
Universal reverse transcription combined with LNA-enhanced and Tm-normalized primers enables accurate and reliable quantification of individual miRNAs from as little as 1 pg total RNA (see figure Accurate quantitation from down to 1 pg total RNA starting material). With exceptional sensitivity and extremely low background, you can accurately quantify very low levels of miRNA without pre-amplification. This makes the miRCURY LNA miRNA PCR System suitable for all types of samples, especially those with low RNA content, such as serum, plasma and other biofluids.
Principle
High-performance reagents for quantitative, real-time RT-PCR

miRCURY LNA RT Kit
This kit includes all the necessary reagents to perform fast and convenient miRNA polyadenylation and reverse transcription in a single reaction step. Using a single, universal RT reaction as the template for subsequent PCR amplifications greatly simplifies the procedure compared to systems that require miRNA-specific, first-strand synthesis (see figure The benefit of a universal reverse transcription step). The UniSp6 RNA spike-in can be used with the control assay – UniSp6 miRCURY LNA miRNA PCR Assay (cat. no. YP00203954) – as a reverse transcription positive control and/or as an inter-plate calibrator in our miRNA PCR panels.

miRCURY LNA SYBR Green PCR Kit
This kit contains the high-performance miRCURY SYBR Green Master Mix for quantitative real-time PCR amplification. The detection is based on SYBR Green, which allows quality control of the resulting PCR amplicon by melting curve analysis. LNA-enhanced primer sets require specific PCR reaction conditions for optimal performance. The kit also includes a control primer set designed for amplification of the synthetic RNA spike-in provided in the miRCURY LNA RT Kit. Refer to the miRCURY LNA RT Kit Handbook for details. The miRCURY LNA miRNA PCR Assays and Panels have been validated using the miRCURY SYBR Green Master Mix; use of other reagents for qPCR will affect the results.
Procedure
The miRCURY LNA miRNA PCR Assay system is an miRNA-specific, LNA-based system designed for sensitive and accurate detection of miRNA by quantitative real-time PCR using SYBR Green. The first step of the procedure is universal reverse transcription, followed by real-time PCR amplification with LNA-enhanced primers (see figure miRCURY LNA miRNA PCR System at a glance).
Applications
The miRCURY LNA RT Kit is used as part of the miRCURY PCR System for:
  • Mature miRNA quantification and profiling
  • Mature miRNA and precursor miRNA detection in parallel
  • snoRNA RNA detection
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