Investigator ESSplex SE Plus Kit

For multiplex amplification of the new European Standard Set of loci, plus SE33, and Amelogenin

  • PCR reaction speed of around 90 minutes
  • Highly sensitive and extremely inhibitor resistant
  • High-quality results due to improved signal-to-noise ratio
  • Straightforward protocols with ready-to-use Fast Reaction Mix
  • Simultaneous amplification of 17 loci

The Investigator ESSplex SE Plus Kit amplifies the full suite of 15 STR markers in the new European Standard Set (ESS) of loci, plus SE33, and the gender-specific Amelogenin, as recommended by the European Network of Forensic Science Institutes (ENFSI) and approved by the European Union Council. The kit utilizes QIAGEN’s fast-cycling technology, enabling amplification in just 90 minutes. The genetic analyzer is calibrated with BT5 Matrix, an optimized dye technology. Highly sensitive, with rapid and reliable results from trace DNA, the Investigator ESSplex SE Plus Kit is suitable for all forensic applications and paternity testing.

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Product Cat. no. List price:
Investigator ESSplex SE Plus Kit (100)
Primer mix, Fast Reaction Mix including HotStarTaq Plus DNA Polymerase, Control DNA, allelic ladder ESSplex SE Plus, DNA size standard 550 (BTO), and nuclease-free water
 381545
S Fr. 3'029.00
Investigator ESSplex SE Plus Kit (400)
Primer mix, Fast Reaction Mix including HotStarTaq Plus DNA Polymerase, Control DNA, allelic ladder ESSplex SE Plus, DNA size standard 550 (BTO), and nuclease-free water
 381547
S Fr. 11'165.00
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The Investigator ESSplex SE Plus Kit is intended for molecular biology applications in forensic, human identity, and paternity testing. This product is not intended for diagnosis, prevention, or treatment of a disease.


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Performance

The Investigator ESSplex SE Plus Kit utilizes fast-cycling technology to provide reliable results in approximately 90 minutes, which is significantly faster than kits from other suppliers.

The novel Fast Reaction Mix is based on proven QIAGEN multiplex PCR technology that has been further optimized to increase inhibitor tolerance. Assay performance tested in the presence of increasing concentrations of 6 types of inhibitor (humic acid, hematin, tannic acid, indigo carmine, calcium, and collagen) shows high sensitivity and superior inhibitor resistance. This enables the generation of reliable, high-quality results.

The Investigator ESSplex SE Plus Kit was validated and evaluated using the GeneAmp 9700 thermal cycler (in standard mode) and the Applied Biosystems 3500 Genetic Analyzer.

The recommended amount of DNA under standard conditions is 0.5 ng. Internal validations demonstrated robust and balanced results with 0.2-2 ng DNA, and reliable results with <0.1 ng DNA.

Principle

The Investigator ESSplex SE Plus Kit uses QIAGEN's fast-cycling PCR technology for the rapid, simultaneous amplification of the 15 polymorphic STR markers that comprise the new European Standard Set (ESS), plus SE33, and Amelogenin. Carefully designed primers prevent overlap of loci alleles, thereby minimizing the likelihood of misinterpretation.

Procedure

The 16 polymorphic STR markers and the gender-specific Amelogenin are amplified simultaneously in a single PCR and labeled with one of the dyes listed in the table "Investigator ESSplex SE Plus Kit dyes and corresponding markers".

The amplified loci then undergo capillary electrophoresis. The results can be analyzed using dedicated software, such as QIAGEN's Investigator IDproof or IDproof Mixture Software. See the table "Technical specifications" for more information.

Investigator ESSplex SE Plus Kit dyes and corresponding markers
DyeMarker
6-FAM Amelogenin TH01 D3S1358 vWA D21S11
BTG D16S539 D1S1656 D19S433 SE33
BTY D10S1248 D22S1045 D12S391 D8S1179 D2S1338
BTR D2S441 D18S51 FGA
Technical specifications
Volume per amplification reaction 25 μl
Matrix BT5
Fluorescence labels 6-FAM, BTG, BTY, BTR, BTO
Genetic analyzers ABI PRISM 310, ABI PRISM 3100/3100-Avant, Applied Biosystems 3130/3130xl, or Applied Biosystems 3500/3500xL Genetic Analyzers
Software for analysis QIAGEN Investigator IDproof or Investigator IDproof Mixture Software, or Applied Biosystems GeneMapper ID, GeneMapper ID-X, or Genotyper Software
Applications

The Investigator ESSplex SE Plus Kit is for rapid and reliable generation of DNA profiles from blood, buccal swabs, and forensic stains, and it is highly suited for:

Criminal database and forensic casework samples
Paternity testing
Population genetics studies
Other human identity testing applications
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Template Files
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(EN) - Investigator Template Files for Genotyper software

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Investigator Template Files for GeneMapper ID-X v9 CODIS Software
For further information on which version of the v9 template files is suitable for your needs, please refer to the Template File Info Note that can also be found in the ''Template File'' section of the product resources.
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Investigator Template Files for GeneMapper ID-X v9 Standard Software
For further information on which version of the v9 template files is suitable for your needs, please refer to the Template File Info Note that can also be found in the ''Template File'' section of the product resources.
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Investigator Template Files for GeneMapper ID v6 Software - (EN)

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