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mericon Quant L. pneumophila Kit

For real-time PCR detection and quantification of Legionella pneumophila in water
  • Highly sensitive and specific detection of L. pneumophila DNA
  • Streamlined detection protocol
  • PCR run time of 73 minutes on the QIAGEN Rotor-Gene Q
  • Full license for PCR without additional costs
The mericon Quant L. pneumophila Kit is designed for the target-specific detection and quantification of Legionella pneumophila DNA in water after concentration (e.g., by filtration). The assay uses a real-time PCR-based protocol and forms part of the comprehensive QIAGEN food testing portfolio, which also features dedicated sample preparation kits and assays for ingredient authentication and GMO DNA detection. The mericon Quant L. pneumophila assay performs optimally on the Rotor-Gene Q, but has also been validated for block thermal cyclers.
Cat No./ID: 290095
mericon Quant L. pneumophila Kit (96)
S Fr.1,718.00
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For 96 reactions: PCR Assay Quant L. pneumophila, Internal Control, Standard DNA, Quant Control DNA, Multiplex PCR Master Mix, QuantiTect Nucleic Acid Dilution Buffer, RNase-free water, 50x ROX Dye Solution

The mericon Quant L. pneumophila Kit is intended for molecular biology applications in food, water, animal feed, and pharmaceutical product testing. This product is not intended for the diagnosis, prevention, or treatment of a disease.

Performance

The mericon Quant L. pneumophila Kit is for the specific detection and quantification of Legionella pneumophila in water after concentration (e.g., by filtration). The assay can detect as few as 10 copies of L. pneumophila DNA in a reaction and it exhibits high specificity for L. pneumophila . In an experiment using 2500 copies of the tested DNA, no cross-reactivity was observed with other pathogens (see the table "Results of mericon Quant L. pneumophila Kit cross-reactivity study").

Results of mericon Quant L. pneumophila Kit cross-reactivity study

 Pathogen  Result  Pathogen  Result
 Legionella pneumophila  +  Legionella erythra  –
 Legionella adelaidensis  –  Legionella feeleii  –
 Legionella impletisoli  –  Legionella jordanis  –
 Legionella israeliensis  –  Legionella tucsonensis  –
 Legionella worsleinsis  –  Legionella beliardensis  –
 Legionella spiritensis  –  Legionella lansingensis  –
 Legionella brunensis  –  Legionella drozanskii  – 
 Legionella geestiana  –  Legionella nautarum  –
 Legionella santicruci  –  Legionella shakespeare  –
 Salmonella abony  –  Bacillus cereus  –
 Clostridium perfringens  –  Campylobacter jejuni  –
 Escherichia coli  –  Yersinia enterocolitica  –
 Staphylococcus aureus  –  Listeria monocytogenes  –
 Shigella flexneri  –  Cronobacter sakazakii  –

Principle

The mericon Quant L. pneumophila Kit is designed for the quantitative detection of Legionella pneumophila in water after filtration. L. pneumophila is the Legionella species most frequently involved in human infections, most notably in the so-called Legionnaires' disease.

Detection of pathogens using real-time PCR is based on the amplification of a specific region of the relevant pathogen genome. The amplified product is detected using target-specific fluorescent probes that bind to it. As the PCR product accumulates, there is an increased fluorescent signal from the bound probes. Monitoring the fluorescence intensities during the PCR run (i.e., in real time) allows the detection of the accumulating PCR product without having to re-open the reaction tubes afterward.

Quantification of the target Legionella species is carried out based on a standard curve covering a concentration range of 25,000–25 copies per PCR reaction. The standard curve is prepared from purified Legionella Standard DNA, which is provided in the mericon Quant L. pneumophila Kit at an accurately defined concentration.

mericon PCR Assays include an internal control to control potential inhibition. This aids in the straightforward interpretation of the results (see the table "Summary of possible PCR outcomes").

The PCR primer set for each assay is highly specific and targets a unique and conserved region of the tested pathogen genome that has been bioinformatically and experimentally verified.

Summary of possible PCR outcomes
Internal control amplifiedTested DNA amplified Meaning
+ + Sample is positive for the target DNA
+ Sample is negative for the target DNA
PCR failed due to inhibition
Procedure

DNA is extracted from a filtered or concentrated water sample using a dedicated sample preparation kit.

The isolated, pure bacterial DNA is assayed using a streamlined real-time PCR protocol that returns results in 73 minutes on QIAGEN's Rotor-Gene Q. The protocol has also been validated for use on other thermal block cyclers.

The results are displayed as easy-to-interpret PCR curves.

Applications
The mericon Quant L. pneumophila Kit is for real-time PCR-based detection and quantification of L. pneumophila in water.
Features
Specifications
Quantitative/qualitative Quantitative
Recommended sample prep QIAamp UCP Pathogen Mini Kit
RUO/CE/ASR RUO
Sample type Water samples after concentration, e.g. filtration
Thermal cycler Rotor-Gene Q and other cyclers
What detected DNA of respective pathogen
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