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cador TKP PCR Reagent

For multiplex detection of T. equigenitalis, K. pneumoniae, and P. aeruginosa DNA

  • Simultaneous multiplex detection of T. equigenitalis, K. pneumoniae, and P. aeruginosa DNA
  • Internal Control to monitor the DNA isolation procedure and PCR inhibition
  • Ready-to-use system for real-time PCR
  • Full license for PCR without additional costs
  • Developed in conjunction with the Veterinary Laboratories Agency (VLA), UK
The cador TKP PCR Kit is a ready-to-use system for the detection of Taylorella equigenitalis, Klebsiella pneumoniae, and Pseudomonas aeruginosa DNA using real-time PCR on Rotor-Gene Systems. All 3 pathogens are detected in one tube on the Rotor-Gene Q. An Internal Control is included in the same tube, to monitor DNA extraction and/or the presence of PCR inhibitors.

For laboratory use. Not for use in veterinary diagnostic procedures. No claim or representation is intended to provide information for the diagnosis, prevention, or treatment of a veterinary disease.



Performance

This PCR assay has been validated as 100% specific for Taylorella equigenitalis , Klebsiella pneumoniae, and Pseudomonas aeruginosa DNA. The validation was carried out at the Veterinary Laboratory Agency (VLA) Weybridge, UK.

Principle

Pathogen detection by the PCR is based on the amplification of specific regions of the pathogen genome. In real-time PCR, the amplified product is detected via fluorescent dyes. These are usually linked to oligonucleotide probes that bind specifically to the amplified product. Monitoring the fluorescence intensities during the PCR run (i.e., in real time) allows the detection of the accumulating product without having to reopen the reaction tubes after the PCR run, thus eliminating possible cross-contamination.

Procedure
The cador TKP PCR Kit is a ready-to-use system. The TKP Master contains reagents and enzymes for the specific amplification of highly conserved regions of the T. equigenitalis, K. pneumoniae, and P. aeruginosa genomes. The amplicons are detected by measuring the green, orange, and crimson fluorescence on a Rotor-Gene Q Real-Time System (see table "Fluorescence channels for the different pathogens" and figure "Detection of the positive control in the green, orange, and crimson channel, respectively").

In addition, the cador TKP PCR Kit contains a second heterologous amplification system to identify possible PCR inhibition. Inhibition is determined by measuring the fluorescence signal in the yellow channel via amplification of an Internal Control (IC) (see figure "Detection of the Internal Control in the yellow channel"). The detection limit of the analytical K. pneumoniae, P. aeruginosa, and T. equigenitalis PCR is not reduced. An external positive control (TKP Control, which contains K. pneumoniae, P. aeruginosa, and T. equigenitalis DNA) is also supplied.

Applications
The cador TKP PCR Kit is a ready-to-use system for the detection of Taylorella equigenitalis, Klebsiella pneumoniae, and Pseudomonas aeruginosa DNA using real-time PCR on Rotor-Gene Systems. All 3 pathogens are detected in one tube on the Rotor-Gene Q. An Internal Control is included in the same tube, to monitor DNA extraction and/or the presence of PCR inhibitors.
Features
Specifications
Quantitative/qualitative Qualitative
Recommended sample prep QIAamp cador Pathogen Mini Kit
RUO/CE/ASR RUO
Sample type null
Thermal cycler Rotor-Gene Q or Applied Biosystems Real-Time PCR cyclers
What detected Taylorella equigenitalis
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