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cador B. hyodysenteriae PCR Reagent

For sensitive and specific detection of Brachyspira hyodysenteriae DNA in samples

  • A ready-to-use system for real-time PCR
  • Highly sensitive detection of B. hyodysenteriae DNA
  • Additional heterologous amplification system to identify possible PCR inhibition
  • Full license for PCR without additional costs
The cador B. hyodysenteriae Reagent specifically amplifies a highly conserved region of the B. hyodysenteriae genome. Additionally, the cador B. hyodysenteriae PCR Reagent contains QIAGEN Internal Control DNA and Internal Control Assay. The Internal Control identifies possible PCR inhibition.

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Cat No./ID: 285215
cador B. hyodysenteriae PCR Reagent (96)
For 96 reactions: cador PCR + IC Reagents, cador B. hyodysenteriae Reagents
For Research Use Only. Not intended for any animal or human therapeutic or diagnostic procedures. No claim or representation is intended to provide information for the diagnosis, prevention or treatment of a disease. Regulatory requirements vary by country; product may not be available in your geographic area.

Performance

This PCR assay has been validated as 100% specific for Brachyspira hyodysenteriae DNA. The validation was carried out at the Veterinary Laboratory Agency (VLA) Weybridge, UK.

Principle

Pathogen identification by PCR is based on the amplification of unique specific regions of the pathogen genome. In real-time PCR, the amplified product is detected via fluorescent dyes. These are usually linked to oligonucleotide probes that bind specifically to the amplified product. Monitoring the fluorescence intensities during the PCR run (i.e., in real time) allows the detection of the accumulating product without having to re-open the reaction tubes after the PCR run.

The cador B. hyodysenteriae PCR Reagent is a ready-to-use system for the identification of DNA from Brachyspira hyodysenteriae using PCR. The kit contains primer-probe sets for specific amplification of a highly conserved region of the Brachyspira hyodysenteriae genome. The kit also contains an additional heterologous amplification system to identify possible PCR inhibition, and an external positive control. Real-time PCR detection can be carried out on the Rotor-Gene Q thermal cycler.

Procedure
Bacterial DNA can be manually isolated using the QIAamp cador Pathogen Mini Kit or the QIAamp DNA Mini Kit. Both isolation protocols are fully automatable on the QIAcube. The isolated DNA is ready for use with the cador B. hyodysenteriae PCR Reagent on the Rotor-Gene Q real-time PCR cycler.
Applications

The cador B. hyodysenteriae PCR Reagent is designed to screen for Brachyspira hyodysenteriae infections in samples.

Features
Specifications
Quantitative/qualitative null
Recommended sample prep null
RUO/CE/ASR null
Sample type null
Thermal cycler null
What detected null

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