text.skipToContent text.skipToNavigation

bactotype MAP PCR Reagent

For identification of DNA from Mycobacterium avium subsp. paratuberculosis


  • Easy-to-use single reaction mix containing controls
  • Internal Control allowing both extraction and amplification to be monitored

The bactotype MAP PCR Reagent enables the safe identification of DNA from Mycobacterium avium subsp. paratuberculosis. The reagent contains primer-probe sets for specific amplification of a highly conserved region of the Mycobacterium avium subsp. paratuberculosis genome. The reagent also contains a heterologous amplification system to allow both nucleic acid extraction and amplification to be monitored, and an external positive control.

For further information, please contact us.
Cat No./ID: 285915
bactotype MAP PCR Reagent (96)
For 96 reactions: Master Mix, Positive Control, Internal Control
For Research Use Only. Not intended for any animal or human therapeutic or diagnostic procedures. No claim or representation is intended to provide information for the diagnosis, prevention or treatment of a disease. Regulatory requirements vary by country; product may not be available in your geographic area.
Principle
This is a real-time PCR reagent for the identification of DNA from Mycobacterium avium subsp. paratuberculosis (MAP). Polymerase chain reaction (PCR) is based on the amplification of specific regions of the pathogen genome. In real-time PCR, the amplified product is identified using fluorescent dyes. These are usually linked to oligonucleotide probes that bind specifically to the amplified product. Monitoring the fluorescence intensities during the PCR run (i.e., in real time) allows the identification of the accumulating product without the need to re-open the reaction tubes afterwards.

Procedure
Prior to real-time PCR, DNA must be extracted from the starting material. QIAGEN offers a range of products for DNA extraction from animal samples.
Applications
The bactotype MAP PCR Reagent is designed to identify Mycobacterium avium subsp. paratuberculosis  DNA in biological samples using polymerase chain reaction (PCR).

fragment fix placeholder