For low-throughput sample disruption for molecular analysis
- Rapid, effective disruption of a range of sample types
- Disposable probes help to minimize cross-contamination
- Time savings through use of disposable probes
- Visual monitoring of disruption using transparent probes
- Seamless integration with QIAGEN sample technologies
The TissueRuptor II is a handheld rotor-stator homogenizer that provides rapid, efficient and flexible disruption of human, animal and plant samples for a wide range of downstream applications. The TissueRuptor II uses transparent disposable probes, which helps to minimize the risk of cross-contamination and enables visual control of the sample disruption process. Use of a disposable probe also provides time savings as the probe does not have to be cleaned after homogenization of each sample.
The TissueRuptor II has all the same features and functionality as the old TissueRuptor.
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TissueRuptor II (230 V, 50/60 Hz, EU/CH)
Handheld rotor–stator homogenizer, 230 V, 50/60 Hz (for Europe [excluding UK and Ireland]), 5 TissueRuptor Disposable Probes
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9002756
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TissueRuptor Disposable Probes (25)
25 nonsterile plastic disposable probes for use with the TissueRuptor II (and the old TissueRuptor)
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990890
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The TissueRuptor II is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
纯化获得高品质基因组DNA,具有可重复性。|对动物组织进行高效的破碎和匀浆。|成功纯化磷蛋白质。|在PCR反应中获得出色表现。|纯化的RNA的RIN值高。|
使用TissueRuptor在裂解缓冲液中以全速破碎冷冻的肝脏和肾脏样本 (各30 mg) 30秒。使用AllPrep DNA/RNA Mini Kit纯化基因组DNA,并进行琼脂糖凝胶电泳分析。M:分子量标准。|使用TissueRuptor以全速破碎下列组织30秒:冷冻的肾、肝和脾 (各10 mg);冷冻的肺 (250 mg);经稳定处理的心脏、肌肉和脑 (各10 mg)。在裂解缓冲液 (肾、肝、脾、肺、心脏和肌肉) 或QIAzol Lysis Reagent (脑) 中破碎样本。使用RNeasy Plus Mini Kit (RNeasy Plus)、RNeasy Midi Kit (RNeasy Midi)、RNeasy Fibrous Tissue Mini Kit (RNeasy Fibrous) 或RNeasy Lipid Tissue Mini Kit (RNeasy Lipid) 纯化总RNA。利用甲醛琼脂糖凝胶电泳分析纯化的DNA。凝胶显示使用TissueRuptor破碎后具有较高的RNA产量和质量。|在350 μl PhosphoProtein Lysis Buffer中以中速破碎冷冻的大鼠肝脏和脑 (各约30 mg) 30–60秒。然后使用1500 μl PhosphoProtein Lysis Buffer稀释匀浆化的样本,置于4°C孵化30分钟,每10分钟短暂涡旋振荡一次。使用PhosphoProtein Purification Kit纯化磷蛋白,并利用western印迹检测。[A] 采用Ponceau S染色转移膜,并进行[B] western印迹。M:分子量标准;FT:流出液;E3:洗脱液部分3。|采用TissueRuptor或传统的转子-定子式匀浆器破碎 [A] 肝脏和 [B] 心脏样本 (各25 mg,冷冻),并使用蛋白酶K裂解1小时,或者不破碎样本,使用蛋白酶K裂解过夜。使用QIAamp DNA Mini Kit纯化DNA。使用HotStarTaq DNA Polymerase和18S核糖体RNA基因特异性引物对洗脱液 (5 μl) 进行PCR。M:GelPilot Mid Range Ladder。|使用带有一次性探头的TissueRuptor或者带有铁制电机探头的传统转子-定子式匀浆器,以全速破碎冷冻的肝脏样本 (各30 mg) 30秒。使用RNeasy Plus Mini Kit纯化总RNA,并在Agilent 2100 Bioanalyzer上分析。高RNA完整性指数 (RIN) 代表了高质量的RNA。|
原理
Genotyping, gene expression and proteomics applications demand effective disruption of biological samples to ensure high yields of DNA, RNA and protein. The TissueRuptor II system delivers thorough and rapid disruption of samples to fully release biomolecules, and also simultaneously homogenizes samples to facilitate subsequent purification procedures using QIAGEN kits (see table).
The TissueRuptor II is an integral part of QIAGEN's complete solution for sample management — from sample collection to purification and analysis of DNA, RNA and protein. Optimized protocols integrate sample disruption with biomolecule purification, enabling a streamlined, efficient workflow. In addition, a range of automated solutions allow purification and analysis of biomolecules at low to high throughputs.
QIAGEN also provides RNAprotect Tissue Reagent (to stabilize RNA) and Allprotect Tissue Reagent (to stabilize DNA, RNA and protein). Tissues collected in these reagents can also be easily disrupted with the TissueRuptor II.
| Analyte purified |
Sample type |
QIAGEN kit |
| RNA |
Easy-to-lyse tissue |
RNeasy Kits
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| RNeasy Plus Kits |
| RNeasy Protect Kits |
| RNA |
Fiber-rich tissue |
RNeasy Fibrous Tissue Kits |
| RNA |
All types of tissue |
RNeasy Lipid Tissue Kits |
| RNeasy Universal Tissue Kits |
| RNA |
Plant tissue |
RNeasy Plant Mini Kit |
| RNA |
Yeast |
RNeasy Kits |
| RNA |
Bacteria |
RNeasy Protect Bacteria Kits |
| microRNA |
All types of tissue |
miRNeasy Kits |
| DNA |
Human tissue |
QIAamp DNA Kits |
| DNA |
Animal tissue |
DNeasy Blood & Tissue Kits |
| Protein |
Tissue |
Qproteome Mammalian Protein Prep Kit |
| Phosphoprotein |
Tissue |
PhosphoProtein Purification Kit |
| Glycoprotein |
Tissue |
Qproteome Glycoprotein Kits |
| DNA and RNA |
Tissue |
AllPrep DNA/RNA Kits |
| DNA, RNA, and protein |
Tissue |
AllPrep DNA/RNA/Protein Mini Kit |
操作流程
The TissueRuptor II is a portable device with a TissueRuptor Disposable Probe. The blade of the probe rotates at a high speed, causing simultaneous disruption and homogenization of a sample through a combination of turbulence and mechanical shearing. Samples that can be processed include human, animal and plant tissues (for plant tissues that are more difficult to disrupt, the TissueLyser II is recommended). Disruption at full speed for as little as 30 seconds is usually sufficient to release nucleic acids or proteins from starting material.
After disruption of a sample, the TissueRuptor Disposable Probe can be discarded, and a new probe can be used to disrupt the next sample. Time is saved and cross-contamination is avoided, as there is no need to clean and reuse the same probe. In addition, the TissueRuptor Disposable Probe is transparent, which allows visual monitoring of the sample disruption process.
应用
The TissueRuptor II enables efficient disruption and homogenization of human, plant and animal samples, including clotted blood. Purification of RNA, DNA, total nucleic acids or protein can then be performed using QIAGEN kits.
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Feature
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Specifications
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Disruption principle
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Simultaneous disruption and homogenization by rotation of the blade of the disposable probe
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Features
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Rapid, effective disruption of a range of sample types. Disposable probes minimize risk of cross-contamination. Time savings through use of disposable probes. Visual monitoring of disruption using transparent probes. Seamless integration with QIAGEN sample technologies.
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Kits compatible with instrument
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All Kits for purification of RNA/DNA/protein
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Technical data
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AUS/EU/UK: 230 V, 144 W, 50/60 Hz, 5000-35,000 rpm; NA: 120 V, 144 W, 50/60 Hz, 5000-35,000 rpm; Japan: 100 V, 125 W, 50/60 Hz, 5000-28,000 rpm
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Technology
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Handheld rotor-stator homogenizer
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Throughput
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1 sample per run
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Typical run time
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15–120 seconds
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For low-throughput disruption of biological samples using disposable probes
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Show details
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Images
Reproducible purification of high-quality genomic DNA.
Frozen liver and kidney samples (30 mg each) were disrupted in lysis buffer at full speed for 30 seconds using the TissueRuptor. Genomic DNA was purified using the AllPrep DNA/RNA Mini Kit and analyzed by agarose gel electrophoresis. M: markers.
Efficient disruption and homogenization of animal tissues.
The following tissues were disrupted at full speed for 30 seconds using the TissueRuptor: frozen kidney, liver, and spleen (10 mg each); frozen lung (250 mg); stabilized heart, muscle, and brain (10 mg each). Samples were either disrupted in lysis buffer (kidney, liver, spleen, lung, heart, and muscle) or QIAzol Lysis Reagent (brain). Total RNA was purified using the RNeasy Plus Mini Kit (RNeasy Plus), the RNeasy Midi Kit (RNeasy Midi), the RNeasy Fibrous Tissue Mini Kit (RNeasy Fibrous), or the RNeasy Lipid Tissue Mini Kit (RNeasy Lipid). Purified RNA was analyzed by formaldehyde agarose gel electrophoresis. The gels show the high yields and quality of the RNA following disruption using the TissueRuptor.
Successful purification of phosphoproteins.
Frozen rat liver and brain (approximately 30 mg each) were disrupted at medium speed for 30–60 seconds in 350 μl PhosphoProtein Lysis Buffer. The homogenized samples were then diluted with 1500 μl PhosphoProtein Lysis Buffer and incubated at 4°C for 30 minutes, with brief vortexing every 10 minutes. Phosphoproteins were purified using the PhosphoProtein Purification Kit and detected by western blotting. [A] Transfer membrane stained with Ponceau S, and [B] western blot. M: markers; FT: flow-through; E3: elution fraction 3.
High performance results in PCR.
[A] Liver and [B] heart samples (25 mg each, frozen) were disrupted with the TissueRuptor or a traditional rotor–stator homogenizer and lysed for 1 hour with proteinase K, or lysed overnight with proteinase K without sample disruption. DNA was purified using the QIAamp DNA Mini Kit. Eluates (5 μl) were subjected to PCR using HotStarTaq DNA Polymerase and primers specific for the 18S ribosomal RNA gene. M: GelPilot Mid Range Ladder.
Pure RNA with high RIN values.
Frozen liver samples (30 mg each) were disrupted at full speed for 30 seconds using either the TissueRuptor with disposable probes or a traditional rotor–stator homogenizer with a steel generator probe. Total RNA was purified using the RNeasy Plus Mini Kit and analyzed on the Agilent 2100 Bioanalyzer. The high RNA Integrity Number (RIN) indicates the high quality of the RNA.
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