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App. Package, M48, Genomic Research

For easy setup of protocols for genomic research applications
  • Easy protocol setup with all protocols on one CD-ROM
  • Optimized protocols help to ensure reliable purification
  • Range of protocols increases processing flexibility

The App. Package, M48, Genomic Research is a CD-ROM containing protocols for DNA purification from cultured cells or soft tissues using the MagAttract DNA Mini M48 Kit on the BioRobot M48 workstation. These protocols provide operating commands for the workstation and allow easy setup through comprehensive, on-screen instructions.

Cat No./ID: 9016148
App. Package, M48, Genomic Research
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Software protocol package for genomic research applications on the BioRobot M48 workstation
The App. Package, M48, Genomic Research is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
Performance

Average yields and purities of six parallel samples per tissue type
TissueAmount Purity* DNA (µg)
Muscle, bovine 40 mg 1.87 ± 0.01 9.0 ± 0.1
Heart, bovine 20 mg 1.87 ± 0.01 12.2 ± 0.6
Spleen, bovine 10 mg 1.88 ± 0.01 27.9 ± 1.1
Lung, bovine 10 mg 1.87 ± 0.01 17.0 ± 1.0
Kidney, bovine 10 mg 1.88 ± 0.01 18.4 ± 0.45
Tail clips, mouse 2 mm 1.88 ± 0.01 18.4 ± 1.4
* Corrected for background at 320 nm.

Average yields and purities of DNA purified from 2 x 106 frozen, cultured cells
Dilution buffer DNA purity (A260/A280)† DNA (µg)
PBS 1.88 ± 0.01 6.84 ± 0.88
1x SSC 1.88 ± 0.01 6.96 ± 0.29
6x SSC 1.86 ± 0.05 7.20 ± 0.29
Lysis buffer 1.89 ± 0.01 7.31 ± 0.21
TE 1.88 ± 0.00 6.08 ± 0.14
† Corrected for background at 320 nm.

Principle

The BioRobot M48 and the MagAttract DNA Mini M48 Kit, with protocols on the App. Package, M48, Genomic Research, reproducibly automate purification of genomic DNA. Purification of 6–48 samples per run is efficient and purified DNA performs well in downstream analyses.

The BioRobot M48 system provides fully automated purification of DNA using silica-coated magnetic particles, eliminating the need for tedious centrifugation steps. The easy-to-use workstation allows purification of 6–48 samples in a single run. Between processing runs, the worktable can be decontaminated using an integrated UV lamp to reduce the chances of environmental contamination.

Procedure
Protocols on the App. Package, M48, Genomic Research, V1.2
Sample type Amount Kit Nucleic acid
Cultured cells 200 µl MagAttract DNA Mini M48 Kit DNA
Tissue (predigested with proteinase K) 200 µl MagAttract DNA Mini M48 Kit DNA
Applications

The high-quality DNA obtained using the MagAttract DNA Mini M48 Kit with protocols in the App. Package, M48, Genomic Research is suitable for use in many genomic research applications, such as:

Genotyping by STR, VNTR, RAPD, NASBA, and AFLP technologies
Sequence and melting-curve analysis
Population genetics
Pharmacogenomic research
Sample to Insight
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