PyroMark Q96 ID Accessories and Reagents

For optimal sample preparation and Pyrosequencing analysis using PyroMark Q96 ID

Reliable quantification of genetic or epigenetic DNA modifications
Solutions for quick and easy sample handling for successful results
Dedicated products for each platform and each step in the workflow
High-quality optimized reagents enable reliable Pyrosequencing results

The PyroMark Q96 ID uses proven Pyrosequencing technology for real-time, sequence-based detection and quantification for genetic analysis and epigenetic methylation studies. PyroMark Q96 ID Accessories and Reagents are high-quality reagents and products that simplify and streamline the Pyrosequencing workflow and enable successful results. PyroMark Q96 ID Accessories and Reagents are designed specifically for use with PyroMark Q96 ID instruments.

Informações sobre pedidos
Detalhes do produto
Recursos do Produto

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Produto	Num Cat.	Preço de lista:
PyroMark Annealing Buffer For annealing of sequencing primer to DNA template	979009	R$414,00	Adicionar ao carrinho
PyroMark Binding Buffer (200 ml) For binding of biotinylated PCR product to Sepharose beads	979006	R$409,00	Adicionar ao carrinho
PyroMark Control Oligo For installation check of system	979203	R$3.328,00	Adicionar ao carrinho
PyroMark CpG Assay (200) PCR and sequencing primers for Pyrosequencing analysis of gene-specific CpG methylation after DNA bisulfite conversion (200 reactions; tube format)	Varies	Details
PyroMark CpG Assay 96 well (200) PCR and sequencing primers for Pyrosequencing analysis of gene-specific CpG methylation after DNA bisulfite conversion (200 reactions; plate format); minimum order of 24 assays per plate	Varies	Details
PyroMark CpG Assay 96 well (50) PCR and sequencing primers for Pyrosequencing analysis of gene-specific CpG methylation after DNA bisulfite conversion (50 reactions; plate format); minimum order of 24 assays per plate	Varies	Details
PyroMark Custom Assay (200) User-designed PCR and sequencing primers for Pyrosequencing analysis (200 reactions; tube format)	Varies	Details
PyroMark Denaturation Sol. (500 ml) For denaturation of double-stranded PCR product into single-stranded DNA template	979007	R$417,00	Adicionar ao carrinho
PyroMark Gold Q96 Reagents (5 x 96) For performing Pyrosequencing reactions on the PyroMark Q96 ID (5 x 96) and PyroMark Q96 MD (15 x 96)	972804	R$3.892,00	Adicionar ao carrinho
PyroMark Gold Q96 SQA Reagents (1 x 96) For performing Pyrosequencing reactions on the PyroMark Q96 ID	972812	Log in
PyroMark OneStep RT-PCR Kit (200) For 200 reactions: QIAGEN OneStep RT-PCR Enzyme Mix, optimized QIAGEN OneStep RT-PCR Buffer, 10x CoralLoad Concentrate, 5x Q-Solution, dNTP Mix, and RNase-Free Water	978803	Log in
PyroMark OneStep RT-PCR Kit (50) For 50 reactions: QIAGEN OneStep RT-PCR Enzyme Mix, optimized QIAGEN OneStep RT-PCR Buffer, 10x CoralLoad Concentrate, 5x Q-Solution, dNTP Mix, and RNase-Free Water	978801	Log in
PyroMark PCR Kit (200) For 200 reactions: 2x PyroMark PCR Master Mix (includes HotStarTaq DNA Polymerase and optimized PyroMark Reaction Buffer containing 3 mM MgCl₂ and dNTPs), 10x CoralLoad Concentrate, 5x Q-Solution, 25 mM MgCl₂, and RNase-Free Water.	978703	R$1.993,00	Adicionar ao carrinho
PyroMark PCR Kit (800) For 800 reactions: 2x PyroMark PCR Master Mix (includes HotStarTaq DNA Polymerase and optimized PyroMark Reaction Buffer containing 3 mM MgCl₂ and dNTPs), 10x CoralLoad Concentrate, 5x Q-Solution, 25 mM MgCl₂, and RNase-Free Water	978705	Log in
PyroMark Q96 Cartridge (3) For delivery of nucleotides and reagents for use with PyroMark Q96 ID	979004	R$3.502,00	Adicionar ao carrinho
PyroMark Q96 Plate (100) For sample analysis of DNA template prepared with magnetic beads; for use with PyroMark Q96 ID	979001	R$1.702,00	Adicionar ao carrinho
PyroMark Q96 Plate Low (100) For sample analysis of DNA template on PyroMark Q96 ID	979002	R$1.702,00	Adicionar ao carrinho
PyroMark Q96 Sample Prep Thermoplate Low Holder for placement of PyroMark Q96 Plate Low on heating block during annealing step prior to PyroMark Q96 ID analysis	9019070	Cotação
PyroMark Q96 Sample Prep Tool Cover (10) Consumables for use with magnetic tool for isolation of single stranded DNA; for use with PyroMark Q96 ID	979003	Log in
PyroMark Q96 Vacuum Prep Trough (5) Reusable plastic troughs for use with all PyroMark Q96 Vacuum Workstations	979011	R$343,00	Adicionar ao carrinho
PyroMark Q96 Vacuum Workstation (100 V) Vacuum workstation for preparing 96 samples in parallel from PCR product to single-stranded template	9001740	Cotação
PyroMark Q96 Vacuum Workstation (110 V) Vacuum workstation for preparing 96 samples in parallel from PCR product to single-stranded template	9001528	Cotação
PyroMark Q96 Vacuum Workstation (220 V) Vacuum workstation for preparing 96 samples in parallel from PCR product to single-stranded template	9001529	Cotação
PyroMark Wash Buffer (conc., 200 ml) For use with the PyroMark Q24 Vacuum Workstation and PyroMark Q96 Vacuum Workstation to wash and neutralize the immobilized DNA	979008	R$416,00	Adicionar ao carrinho

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PyroMark Q96 ID Accessories and Reagents are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.

Aplicações

PyroMark Q96 ID Accessories and Reagents enable Pyrosequencing analysis with the PyroMark Q96 ID instrument for applications such as:

HRM
Methylation analysis
Mutation analysis
SNP analysis

FAQs

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	What concentration should be used for the sequencing primer in pyrosequencing? FAQ ID -2826	Visualizar
	Can I order the nucleotides from PyroMark Gold Reagents separately? FAQ ID -2827	Visualizar
	Which purity grade is recommended for pyrosequencing primers? FAQ ID -2832	Visualizar
	How many times can vacuum troughs be re-used with the PyroMark Vacuum Preparation Stations? FAQ ID -2848	Visualizar
	Where can I order the Streptavidin Sepharose beads for pyrosequencing? FAQ ID -2850	Visualizar
	How many nucleotides of a homopolymer can be resolved in pyrosequencing? FAQ ID -2871	Visualizar
	Can unused wells in a pyrosequencing plate be used in the next run? FAQ ID -2872	Visualizar
	What is the reason for signals ceasing in the middle of a pyrosequencing run? FAQ ID -2875	Visualizar
	How do I reduce background peaks in the pyrosequencing pyrogram? FAQ ID -2877	Visualizar
	How do I prevent a drifting baseline in my pyrosequencing pyrogram? FAQ ID -2878	Visualizar
	What is the reason for a high substrate peak in the pyrosequencing pyrogram? FAQ ID -2879	Visualizar
	What is the reason for split peaks appearing in between dispensations on my pyrosequencing pyrogram? FAQ ID -2881	Visualizar
	How accurate and reliable is PyroMark Q24 in mutation analysis? FAQ ID -2094	Visualizar
	Which kits can be used in combination with the PyroMark CpG Assays and PyroMark Custom Assays? FAQ ID -2822	Visualizar
	What kind of shaker should be used for the pyrosequencing binding step? FAQ ID -2837	Visualizar
	Which heating block is recommended for the pyrosequencing annealing step? FAQ ID -2838	Visualizar
	Can PyroMark Gold reagents be vortexed? FAQ ID -2844	Visualizar
	What is the concentration of PyroMark Control Oligo? FAQ ID -2846	Visualizar
	What should be the single peak height for the PyroMark Control Oligo on the different PyroMark instruments? FAQ ID -2852	Visualizar
	What is the use of the PyroMark Q24 Validation Oligo? FAQ ID -2855	Visualizar
	How many times can the cartridges for Pyromark Q24 or PyroMark Q96 ID instruments are reused? FAQ ID -2863	Visualizar
	What is the purpose of the unmethylated and unconverted control DNA of the EpiTect PCR Control DNA Set? FAQ ID -2007	Visualizar
	How do I set up a PyroMark CpG Assay? FAQ ID -2814	Visualizar
	How are the PyroMark CpG Assays shipped and stored? FAQ ID -2816	Visualizar
	How are the PyroMark CpG Assays reconstituted? FAQ ID -2817	Visualizar
	Does QIAGEN offer a design of Custom PyroMark CpG Assays? FAQ ID -2818	Visualizar
	What are the features of PyroMark CpG Assays e.g. in terms of design, validation? FAQ ID -2821	Visualizar
	Will the primer sequence for the PyroMark CpG Assay be provided? FAQ ID -2823	Visualizar
	In which format can PyroMark CpG Assays be ordered? FAQ ID -2824	Visualizar
	What is the recommended amplicon size for CpG assays? FAQ ID -2825	Visualizar
	Is the CpG software included in the PyroMark instruments to study methylation status? FAQ ID -2842	Visualizar
	What is included in a PyroMark Custom Assay? FAQ ID -2815	Visualizar
	Which end of the PCR primer for pyrosequencing should be biotinylated? FAQ ID -2839	Visualizar
	Will dUTP in a PCR reaction affect pyrosequencing? FAQ ID -2843	Visualizar
	Which PyroMark Gold Q96 Reagent should be used for which instrument and application? FAQ ID -2836	Visualizar
	What is the meaning of the abbreviations NDT and CDT and what are the differences? FAQ ID -2841	Visualizar
	How many times can the CDTs, NDTs, and RDTs be used? FAQ ID -2865	Visualizar
	Does the Pyromark Assay Design or application software give any cycling conditions for individual assay primers or a PCR setup pipetting scheme? FAQ ID -2862	Visualizar

Safety Data Sheets

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	Safety Data Sheets Download Safety Data Sheets for QIAGEN product components.	Visualizar
	Safety Data Sheets Download Safety Data Sheets for QIAGEN product components.	Visualizar

Brochures & Guides

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	Product Profile - PyroMark Q24 Advanced	Mostrar detalhes
	Pyrosequencing – the synergy of sequencing and quantification	Mostrar detalhes

Kit Handbooks

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	PyroMark Control Oligo Handbook For use with PyroMark Q48 Autoprep, PyroMark Q24 Advanced, PyroMark Q24, PyroMark Q96 ID and PyroMark Q96 MD systems	Mostrar detalhes
	(EN) - PyroMark Gold Q96 Reagents Handbook For performing Pyrosequencing reactions on the PyroMark Q96 ID, PyroMark Q96 MD, and PyroMark Q96 MD Automated	Mostrar detalhes
	(EN) - PyroMark OneStep RT-PCR Handbook	Mostrar detalhes
	(EN) - PyroMark PCR Handbook	Mostrar detalhes
	(EN) - PyroMark Q96 Cartridge Product Sheet	Mostrar detalhes

Additional Resources

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	(EN) - Offline Ordering Instructions for PyroMark CpG Assays	Mostrar detalhes
	(EN) - Offline Ordering Instructions for PyroMark Custom Assays	Mostrar detalhes

Scientific Posters

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	(EN) - PyroMark CpG Assays: A new tool for genome-wide methylation profiling by Pyrosequencing	Mostrar detalhes
	Advanced DNA methylation analysis for verification of Infinium HumanMethylation450 BeadChip data Scientific poster	Mostrar detalhes

User-Developed Protocols

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	Pyrosequencing® method for the forensic identification of tissue source using QIAGEN’s PyroMark® Q24 Advanced system - (EN)	Mostrar detalhes

White Papers

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	(EN) - Techniques to overcome bottlenecks in epigenetics research	Mostrar detalhes

Application Notes

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	Tissue source attribution using the PyroMark Q48 Autoprep System: Sperm identification in forensic casework	Mostrar detalhes
	Pyrosequencing – tissue source forensic identification using PyroMark Q24 Advanced A Pyrosequencing method for the forensic identification of tissue source using QIAGEN’s PyroMark Q24 Advanced system.	Mostrar detalhes

Quick-Start Protocols

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	(EN) - PyroMark PCR Kit Quick-Start Protocol	Mostrar detalhes
	PyroMark Q96 Vacuum Workstation Quick-Start Guide - (EN)	Mostrar detalhes

Fichas de Segurança

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	MSDS PyroMark Annealing Buffer
	MSDS PyroMark Binding Buffer (200 ml)
	MSDS PyroMark Control Oligo
	MSDS PyroMark CpG Assay (200)
	MSDS PyroMark CpG Assay 96 well (200)
	MSDS PyroMark CpG Assay 96 well (50)
	MSDS PyroMark Custom Assay (200)
	MSDS PyroMark Denaturation Sol. (500 ml)
	MSDS PyroMark Gold Q96 Reagents (5 x 96)
	MSDS PyroMark Gold Q96 SQA Reagents (1 x 96)
	MSDS PyroMark OneStep RT-PCR Kit (200)
	MSDS PyroMark OneStep RT-PCR Kit (50)
	MSDS PyroMark PCR Kit (200)
	MSDS PyroMark PCR Kit (800)
	MSDS PyroMark Wash Buffer (conc., 200 ml)
	CofA

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