QIAamp Viral RNA Mini Kit

For isolation of viral RNA from cell-free body fluids
  • Rapid isolation of high-quality, ready-to-use RNA
  • No organic extraction or alcohol precipitation
  • Consistent, high yields
  • Complete removal of contaminants and inhibitors

The QIAamp Viral RNA Mini Kit simplifies purification of viral RNA from cell-free body fluids with fast spin-column or vacuum procedures. Viral RNA binds specifically to the QIAamp silica membrane, and pure viral RNA is eluted in either water or a buffer provided with the kit. Purification can be fully automated on the QIAcube Connect.

The QIAamp Viral RNA Mini Kit (50) is temporarily unavailable. Please consider using the QIAamp Viral RNA Mini Kit (250), Kat. No. 52906 instead.

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产品 货号 目录价:
QIAamp Viral RNA Mini Kit (250)
IMPORTANT: Packaging of this kit is optimized for the global fight against COVID19 caused by the coronavirus (SARS-CoV-2). The only kit format available is the 250 prep version. See Important Note for details.

For 250 RNA preps: 250 QIAamp Mini Spin Columns, Carrier RNA, Collection Tubes (2 ml), RNase-free buffers
52906
£892.00
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Buffer AVE (108 x 2 ml)
Buffer AVE (108 x 2 ml)
1020953
£312.00
加入购物车

QIAamp Viral RNA Mini Kit适用于分子生物学应用。该产品不适用于疾病的诊断、预防或治疗。


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扩增血浆中的RNA。|
从血浆中纯化的1026 nt RNA片段的RT-PCR产物。将10倍梯度稀释液(如图所示)加入血浆,使用QIAamp Viral RNA Mini Kit纯化。M:分子量标准;C:阴性对照。|
性能
The high-quality viral RNA isolated using the QIAamp Viral RNA Mini Kit performs well in a wide range of downstream applications, including viral genotyping, viral epidemiology, and infectious disease research (see figure "Amplification of RNA from plasma").
原理
The QIAamp Viral RNA Mini Kit simplifies isolation of viral RNA from cell-free body fluids with fast spin-column or vacuum procedures. No phenol–chloroform extraction is required. Viral RNA binds specifically to the QIAamp silica membrane while contaminants pass through. PCR inhibitors, such as divalent cations and proteins, are completely removed in two efficient wash steps, leaving pure viral RNA to be eluted in either water or a buffer provided with the kit.

QIAamp RNA technology yields viral RNA from cell-free body fluids ready to use in RT-PCR and blotting procedures. QIAamp sample preparation technology is fully licensed.

操作流程

Optimized buffers and enzymes lyse samples, stabilize nucleic acids, and enhance selective RNA adsorption to the QIAamp membrane. To guarantee RNA integrity, samples are lysed under highly denaturing conditions to inactivate RNases. Alcohol is added and lysates loaded onto the QIAamp spin column. Wash buffers are used to remove impurities and pure, ready-to-use RNA is then eluted in water or low-salt buffer.

The QIAamp Viral RNA Mini Kit is automatable on the QIAcube. The QIAamp Viral RNA Mini Accessory Set provides the additional buffers and reagents needed for automated, low-throughput sample prep using both QIAcube and QIAamp Viral RNA Mini Kit.

Vacuum processing

For greater speed and convenience in RNA purification, samples can be processed by vacuum instead of centrifugation. QIAamp Mini spin columns (see figure "QIAamp Viral RNA Mini spin column") are accommodated on the QIAvac 24 manifold using VacValves and VacConnectors, provided in the QIAamp Vac Accessory Set. VacValves should be used if sample flow rates differ significantly, in order to ensure consistent vacuum. Disposable VacConnectors are used to avoid any cross-contamination.

应用

The QIAamp Viral RNA Mini Kit simplifies purification of viral RNA from cell-free body fluids, offering fast spin-column or vacuum procedures, or automation on QIAcube. Sample types include:

Plasma and serum
CSF
Urine
Other cell-free body fluids
Cell-culture supernatants
特点
参数
应用 PCR, qPCR, real-time PCR
洗脱体积 50 µl
规格 Spin columns
主要样本类型 Liquid media
处理 Manual (centrifugation or vacuum)
纯化总RNA、miRNA、poly A+ mRNA、DNA或蛋白 Viral RNA
样本量 140 µl
技术 Silica technology
每次运行或制备时间 20–40 minutes
产量 >90% recovery

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For purification of viral RNA from plasma, serum, cell-free body fluids, cell-culture supernatants
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图片
Amplification of RNA from plasma
扩增血浆中的RNA。
从血浆中纯化的1026 nt RNA片段的RT-PCR产物。将10倍梯度稀释液(如图所示)加入血浆,使用QIAamp Viral RNA Mini Kit纯化。M:分子量标准;C:阴性对照。