What is the longest fragment that was cloned with the pQE UA Cloning Vector?
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The longest fragment we have cloned with this system was about 3 kb. However, some of our customers have successfully cloned 5 kb fragments with the pDrive Cloning Vector of the QIAGEN PCR Cloning Kit. Since the two systems are very similar, we would encourage you to give it a try if you have fragments longer than 3 kb. When using such long inserts, ligation time should be at least 2 hours to increase cloning efficiency.