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RNeasy PowerMicrobiome Kit (50)
Cat. No. / ID: 26000-50
For the isolation of total RNA from stool and gut material
KitSolution
RNeasy PowerMicrobiome Kit
Solution
This product contains substances regulated under REACH (EC 1907/2006 Annex XIV). The use of this product in the EU is permitted subject to an exemption (Article 56(3)). Please refer to the REACH notification and the SDS of this product, both of which can be found in the “Resources” section of this page, for more information.
The RNeasy PowerMicrobiome Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
✓ 24/7 automatic processing of online orders
✓ Knowledgeable and professional Product & Technical Support
✓ Fast and reliable (re)-ordering
Features
- Fast and easy protocol isolates high-quality total RNA in less than 45 minutes
- Efficient isolation of total RNA from difficult microbiome samples
- Highly pure RNA isolation with Inhibitor Removal Technology
- Simple co-isolation of miRNA and DNA using an on-column procedure
Product Details
Get high-quality RNA fast, free of lingering PCR inhibitors with the RNeasy PowerMicrobiome Kit. Designed for purification of total RNA from stool, dried feces, gut material, contaminated buccal swabs and secretions, the RNeasy Power Microbiome Kit isolates RNA that is ready for demanding downstream applications including RT-PCR, qPCR and RNA-seq. Digested food, heme from lysed red blood cells in stool and other PCR inhibitors are no match for the Inhibitor Removal Technology that is included in the kit. The RNeasy PowerMicrobiome Kit also isolates miRNA and can optionally co-isolate DNA using a simple, on-column procedure using reagents provided with the kit. Want to try this solution for the first time? Request a quote for a trial kit.
Isolation of RNA using the RNeasy PowerMicrobiome Kit can be automated on the QIAcube Connect.
RNeasy PowerMicrobiome Kit was formerly sold by MO BIO as PowerMicrobiome RNA Isolation Kit.
Supporting data and figures
Figure 1. High-quality, intact total RNA isolated from human stool using the RNeasy PowerMicrobiome Kit.
Healthy volunteer samples were collected and total RNA purified using the RNeasy PowerMicrobiome Kit. Profiling and quantification of 1 µl of RNA was performed using an Agilent Bioanalyzer RNA Nano Kit chip. In the electropherogram, clear 16S and 23S bands are shown for prokaryotic rRNA. RNA processed through the RNeasy PowerMicrobiome Kit was demonstrated to be high-quality by an RIN of 9.4. (Data kindly provided by C. Vincent, McGill University, Montreal, Canada.)
Specifications
| Features | Specifications |
| Sample types | Stool, gut material, dried feces, contaminated buccal swabs and secretions |
| Format | Silica Spin Filter Tubes |
| Time per run or per prep | 45 minutes |
| Sample size | 0.25 g |
| Throughput | 1-24 samples |
| Bead size | 0.1 mm glass |
| Storage temperature | Store lyophilized DNase I at 4°C All other kit reagents and components should be stored at room temperature (15-30°C) |
Resources
Safety Data Sheets (7)
Quick-Start Protocols (1)
Additional Resources (1)
Application Notes (1)
Brochures & Guides (1)
Kit Handbooks (1)