CompactPrep Plasmid Kits enable fast, large-scale plasmid purification. The use of a vacuum manifold allows up to 24 samples to be purified in parallel on a single workbench (see figure "Microcentrifuge column format") for plasmid midi and plasmid maxi preps. Up to 200 µg plasmid DNA is purified in less than 60 minutes from 25 ml (high-copy plasmids) or 50 ml (low-copy plasmids) LB medium using the CompactPrep Plasmid Midi Kit. DNA is eluted in 100 µl and concentrations are typically 1–2 µg/µl. The CompactPrep Plasmid Maxi Kit enables purification of up to 750 µg plasmid DNA in less than 60 minutes from 100 ml (high-copy plasmid) or 250 ml (low-copy plasmid) LB medium. DNA is eluted in 200 µl and concentrations are typically 3–4 µg/µl. CompactPrep Plasmid Kits provide molecular biology grade plasmid DNA with low endotoxin levels (see figure "Low endotoxin levels").
Maximum plasmid yields using LyseBlue
LyseBlue reagent is now also included in CompactPrep Kits ensuring high DNA yields. Using a simple visual identification system, LyseBlue reagent prevents common handling errors that lead to inefficient cell lysis and incomplete precipitation of SDS, cell debris and genomic DNA. LyseBlue is optional and provided in a separate vial within the kit.