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ipsogen JAK2 MutaScreen Kits CE

For qualitative or semi-quantitative detection of the JAK2 V617F/G1849T mutation using real-time PCR
  • Highly reliable qualitative or semi-quantitative detection
  • Multiplex analysis with no need for optimization
  • Compliance with EU IVD Directive 98/79/EC
  • Easy workflow with ready-to-use solutions
The ipsogen JAK2 MutaScreen Kits are in vitro molecular diagnostic kits for real-time PCR on the Rotor-Gene Q and other real-time PCR instruments. The kits provide reagents optimized for reliable and sensitive detection, and qualitative or semi-quantitative analysis of the JAK2 V617F/G1849T mutation in genomic DNA extracted from peripheral blood of subjects with suspected myeloproliferative neoplasm (MPN).
Cat No./ID: 673022
ipsogen JAK2 MutaScreen Kit (10) CE
For 10 reactions: V617F Positive Control, V617F Negative Control, V617F Cut-Off Sample, Primers and Probes Mix JAK2 wild-type and JAK2 V617F
Cat No./ID: 673023
ipsogen JAK2 MutaScreen Kit (24) CE
For 24 reactions: V617F Positive Control, V617F Negative Control, V617F Cut-Off Sample, Primers and Probes Mix JAK2 wild-type and JAK2 V617F
Performance
To ensure highest accuracy, ipsogen JAK2 MutaScreen Kits have been optimized to detect the JAK2 V617F/G1849T mutation and use DNA positive and negative controls for qualitative analysis (see figures Representative allelic discrimination experiments for qualitative detection of the JAK2 V617F/G1849T mutation in genomic DNA and Representative allelic discrimination experiments for semi-quantitative detection of the JAK2 V617F/G1849T mutation in genomic DNA). ipsogen JAK2 MutaScreen Kits provide a DNA 2% cut-off sample (COS) positivity and a minimum of 25 ng of input sample DNA is recommended.
Principle
ipsogen JAK2 MutaScreen Kits are ready-to-use kits for the detection, qualitative, or semi-quantitative analysis of the JAK2 V617F/G1849T somatic mutation due to a single nucleotide polymorphism (SNP) in genomic DNA extracted from peripheral blood of subjects with suspected myeloproliferative neoplasm (MPN) (see figure JAK2 mutation site identification) . The kits provide highly reliable and reproducible results. ipsogen JAK2 MutaScreen Kits and the ipsogen JAK2 MutaScreen EZ Kit provide a DNA cut-off sample (COS), a DNA positive control (PC), and a negative control (NC) for JAK2 V617F. Use of the cut-off sample and controls enables accurate qualitative analysis of genotyping results. The ipsogen JAK2 MutaScreen EZ Kit also includes real-time PCR master mix. The ipsogen JAK2 MutaScreen RS Kit provides a V617F reference scale.
Procedure
The first step is extraction and determination of quality and quantity of genomic DNA. The second step is the amplification of DNA by real-time PCR. Using the ipsogen JAK2 MutaScreen Kits provide reliable detection, qualitative, or semi-quantitative analysis of the JAK2 V617F/G1849T mutation. Simply start the reaction using the optimized protocols described in the kit handbook.
Applications
ipsogen JAK2 MutaScreen Kits enable accurate and reliable detection, qualitative, or semi-quantitative analysis of the JAK2 V617F/G1849T mutation for in vitro diagnostic use.
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