EZ1 RNA Tissue Mini Kit

For automated purification of high-quality total RNA
  • Purification of up to 30 µg total RNA
  • Reliable processing of up to 10 mg easy-to-lyse tissues
  • RNase-free setup for reliable results
  • Scalable elution volumes
  • Easy protocol setup with credit-card ease of use
The EZ1 RNA Tissue Mini Kit contains all required reagents and labware for automated purification of total RNA from up to 10 mg standard tissue samples using magnetic particle technology. Reagents are supplied in prefilled reagent cartridges, which ensures speed and convenience in loading the instrument. The EZ1 RNA Tissue Kit can be used with the EZ1 Advanced XL, the EZ1 Advanced, or the BioRobot EZ1.
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产品 货号 目录价:
EZ1 RNA Tissue Mini Kit (48)
For 48 preps: Reagent Cartridges (Tissue RNA), Disposable Filter-Tips, Disposable Tip-Holders, Sample Tubes (2 ml), Elution Tubes (1.5 ml), Buffer RLT, RNase-free DNase I, RNase-free water
959034
询价

EZ1 RNA Tissue Mini Kit适用于分子生物学应用。该产品不适用于疾病的诊断、预防或治疗。


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高效的纯化,产量具有可重复性。|高纯度RNA,具有可重复性。|
使用EZ1 RNA Tissue Mini Kit从大鼠肾脏和肝脏各10 mg的样本、以及大鼠胰腺、脾和肺各5 mg样本中纯化总RNA。每种类型样本各三份。通过吸光度(A260)测定总RNA产量,已校正背景(A320)。CV肾脏= 15%; CV肝脏= 1%; CV胰腺= 8%; CV脾= 10%; CV肺= 22%。|使用EZ1 RNA Tissue Mini Kit,从4种类型的大鼠组织(每种类型2–3个样本)中纯化RNA。纯化的总RNA通过分光光度法检测。[A] 肾脏10 mg;[B] 肝脏10 mg;[C] 胰腺5 mg;[D] 脾5 mg。|
性能

The yields and high quality of total RNA purified using the EZ1 RNA Tissue Mini Kit are consistent and reproducible (see figures "Efficient purification and reproducible yields" and "Consistent highly pure RNA").

原理

Automated purification with the EZ1 RNA Tissue Mini Kit requires use of the EZ1 Advanced XL and the EZ1 Advanced XL RNA Card, or the EZ1 Advanced and the EZ1 Advanced RNA Card, or the BioRobot EZ1 and the EZ1 RNA Card. EZ1 cards are preprogrammed with protocols that guide the user through worktable setup and provide the instrument with commands for the purification procedure.

Using silica-coated magnetic-particle technology, the easy-to-use instrument allows purification of 1-14 samples (EZ1 Advanced XL) or 1-6 samples (EZ1 Advanced and BioRobot EZ1) in a single run. Presealed reagent cartridges and the automatic sensor-controlled lock of the instrument door reduce the chances of environmental contamination or degradation due to RNases. The automated procedure provides high repeatability and linear yields over a range of starting amounts. The flexibility to purify high-quality total RNA from a wide range of tissue types ensures that samples can be purified for a range of gene expression analysis applications. RNA yield using the EZ1 RNA Tissue Mini Kit is dependent upon many variables, including sample weight, tissue type and developmental state.

操作流程

Tissues are first lysed and homogenized in Buffer RLT. RNA is then purified in one step on the instrument following the automated EZ1 RNA procedure. For certain RNA applications that are sensitive to very small amounts of DNA, an integrated DNase digestion step removes genomic DNA contamination. Total RNA is eluted in 200 µl, 100 µl, or 50 µl, ready to use in all downstream applications.

应用

The high-quality RNA obtained using the EZ1 RNA Tissue Mini Kit is suited for use in many applications, such as:

  • Gene expression analysis using real-time RT-PCR technologies
  • Gene expression analysis using microarray technologies
  • Oncology and pathology research
特点
参数
应用 PCR, real-time RT-PCR, microarray
洗脱体积 50–200 µl
自动化处理 BioRobot EZ1
规格 Sample tube
主要样本类型 Tissue samples
处理 Automated
纯化总RNA、miRNA、poly A+ mRNA、DNA或蛋白 RNA
样本量 5–10 µg
稳定 Yes
技术 Magnetic particles
每次运行或制备时间 40 minutes
产量 <30 µg

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EZ1 RNA Cell Mini Kit
For purification of total RNA from human and animal cells using EZ1instruments
EZ1 RNA Tissue Mini Kit
For purification of total RNA from easy-to-lyse human and animal tissues using EZ1 instruments
EZ1 RNA Universal Tissue Kit
For purification of total RNA from any type of human and animal tissue using EZ1 instruments
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图片
Efficient Purification and Reproducible Yields
高效的纯化,产量具有可重复性。
使用EZ1 RNA Tissue Mini Kit从大鼠肾脏和肝脏各10 mg的样本、以及大鼠胰腺、脾和肺各5 mg样本中纯化总RNA。每种类型样本各三份。通过吸光度(A260)测定总RNA产量,已校正背景(A320)。CV肾脏= 15%; CV肝脏= 1%; CV胰腺= 8%; CV脾= 10%; CV肺= 22%。
Consistent Highly Pure RNA
高纯度RNA,具有可重复性。
使用EZ1 RNA Tissue Mini Kit,从4种类型的大鼠组织(每种类型2–3个样本)中纯化RNA。纯化的总RNA通过分光光度法检测。[A] 肾脏10 mg;[B] 肝脏10 mg;[C] 胰腺5 mg;[D] 脾5 mg。