EZ1 DNA Blood 200 µl Kit

For automated purification of DNA from 1–6 samples or 1–14 samples per run
  • Rapid purification of up to 6 µg high-quality DNA
  • Processing of up to 200 µl whole blood samples
  • Easy protocol setup with credit-card ease of use
  • Standardized processing ensures reproducible results

The EZ1 DNA Blood 200 μl Kit contains all required reagents and labware for rapid, automated purification of DNA from blood samples of up to 200 μl using magnetic particle technology. The kit can be used with the EZ1 Advanced XL (1–14 samples per run), EZ1 Advanced (1–6 samples per run), and BioRobot EZ1 (1–6 samples per run) instruments. Reagents are supplied in prefilled reagent cartridges, which ensures speed and convenience in loading the instrument.

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产品 货号 目录价:
EZ1 DNA Blood 200 µl Kit (48)
For 48 preps: Reagent Cartridge (Blood 200 µl), Disposable Filter-Tips, Disposable Tip-Holders, Sample Tubes (2 ml), Elution Tubes (1.5 ml)
951034
询价

EZ1 DNA Blood 200 µl Kit适用于分子生物学应用。该产品不适用于疾病的诊断、预防或治疗。


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从全血中纯化高品质DNA。|高品质DNA用于灵敏、特异性分析。|
使用EZ1 DNA Blood 200 µl Kit(上方条带)从200 µl血液样本中纯化DNA,使用EZ1 DNA Blood 350 µl Kit(下方条带)从350 µl血液样本中纯化DNA。泳道1–6和7–12分别来自EZ1仪器上的8次运行的第一次和最后一次。M:1000 bp DNA分子量标准(100 ng);+:阳性对照;:阴性对照。|使用从全血中纯化获得的DNA进行单拷贝MECL-1基因的PCR分析。从ACD、EDTA、和肝素保存的人全血样本中,使用EZ1 DNA Blood 200 μl Kit纯化基因组DNA。对模板DNA进行梯度稀释。将纯化的DNA的等分试样(体积如图所示)用于每50 μl的PCR反应中。|
性能
The automated procedure provides high repeatability (see figure "High-quality DNA from whole blood") and linear yields over a range of starting amounts. DNA purified using the EZ1 DNA Blood 200 µl Kit gives high-performance results in downstream applications, such as PCR (see figure "High-quality DNA for sensitive and specific analyses").
原理

Automated purification with the EZ1 DNA Blood 200 µl Kit requires use of the EZ1 Advanced XL and the EZ1 Advanced XL DNA Blood Card, or the EZ1 Advanced and the EZ1 Advanced DNA Blood Card, or the BioRobot EZ1 and the EZ1 DNA Blood Card. EZ1 cards are preprogrammed with protocols that guide the user through worktable setup and provide the instrument with commands for the purification procedure. Using silica-coated magnetic-particle technology, the easy-to-use instrument allows purification of 1–14 samples (EZ1 Advanced XL) or 1–6 samples (EZ1 Advanced and BioRobot EZ1) in a single run. Presealed reagent cartridges and the automatic sensor-controlled lock of the instrument door reduce the chances of environmental contamination.

操作流程

Whole blood samples (200 µl) are loaded onto the instrument, and genomic DNA is purified in one step following the automated EZ1 DNA procedure. Genomic DNA is eluted in 200 µl, 100 µl, or 50 µl volumes, ready to use in all downstream applications, such as PCR.

应用

The high-quality DNA obtained using the EZ1 DNA Blood 200 μl Kit is suited for use in many applications, such as:

Population genetics and pharmacogenomic research
PCR and real-time PCR
Genotyping or sequencing
Oncology research
SNP, STR, VNTR, RAPD, NASBA, and AFLP technologies
特点
参数
应用 PCR, real-time RT-PCR, genotyping
洗脱体积 200 µl
自动化处理 BioRobot EZ1
规格 Sample tube
主要样本类型 Whole blood
处理 Automated
纯化总RNA、miRNA、poly A+ mRNA、DNA或蛋白 DNA
样本量 200 µl
技术 Magnetic particles
每次运行或制备时间 15–20 minutes
产量 2.6–6 µg

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产品介绍与指南
1
Fully automated low- to medium-throughput purification of nucleic acids
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试剂盒操作手册
1
EZ1 DNA Blood 200 μl Kit
EZ1 DNA Blood 350 μl Kit
For automated purification of DNA from blood and buffy coat using EZ1 instruments
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图片
High-Quality DNA from Whole Blood
从全血中纯化高品质DNA。
使用EZ1 DNA Blood 200 µl Kit(上方条带)从200 µl血液样本中纯化DNA,使用EZ1 DNA Blood 350 µl Kit(下方条带)从350 µl血液样本中纯化DNA。泳道1–6和7–12分别来自EZ1仪器上的8次运行的第一次和最后一次。M:1000 bp DNA分子量标准(100 ng);+:阳性对照;:阴性对照。
High-Quality DNA for Sensitive and Specific Analyses
高品质DNA用于灵敏、特异性分析。
使用从全血中纯化获得的DNA进行单拷贝MECL-1基因的PCR分析。从ACD、EDTA、和肝素保存的人全血样本中,使用EZ1 DNA Blood 200 μl Kit纯化基因组DNA。对模板DNA进行梯度稀释。将纯化的DNA的等分试样(体积如图所示)用于每50 μl的PCR反应中。