Get the most out of NGS variant panels – comparing single primer extension (SPE) to a two-primer amplicon approach
NGS panels have the power to give researchers deeper insights into the genes or genomic regions that matter most. However, issues with coverage gaps, primer drop out and errors during amplification can complicate results.
In this webinar, we’ll take a look at how panel design affects specificity, uniformity and coverage. We’ll discuss the difference between a two-primer amplicon and single primer extension approach, and compare how using unique molecular indices (UMIs) to tag original molecules enhances the ability to call low-frequency variants. We’ll also review a head-to-head comparison of both approaches and discuss the application example. Join us for a comprehensive review on how to get the most out of your NGS sequencing panels!
About the speaker
Raed Samara, Ph.D, Senior Global Product Manager
Raed Samara, PhD is a Global Product Manager for NGS technologies at QIAGEN, with a focus on pre-analytics and targeted enrichment. Prior to joining QIAGEN, he was a postdoctoral fellow at the National Cancer Institute conducting research in the field of cancer immunology with emphasis on identifying strategies to boost the efficacy of cancer vaccines. He received his Ph.D. degree from Georgetown University in tumor biology.
Next Generation Sequencing
Molecular Biology Research