Liquid biopsy webinars on-demand

Exosomes and microvesicles function as tiny shuttles for transferring information between donor and recipient cells. These small vesicles have recently attracted a tremendous amount of attention from the scientific community. In this webinar, we discuss the current understanding of their biogenesis, molecular phenotype and functional mechanisms.

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Methods for purifying exosomes for RNA isolation have been time-consuming and inconsistent. QIAGEN has developed solutions to help isolate and enrich exosomes and other extracellular vesicles, to allow the detection of low-abundance RNAs that are in circulation. The seminar will describe new solutions for sample preparation and discuss how to maximize quantity and purity of low-abundance fragments.

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Exosomes and other extracellular vesicles (EVs) such as microvesicles carry functional cargo and play an important role in disease progression. Recently, exosomal RNAs, especially microRNAs, have attracted tremendous interest as potential circulating diagnostic biomarkers. This webinar presents an integrated system for identification and characterization of specific RNA molecules from exosomes and other extracellular vesicles (EVs). Advanced assay technologies for characterization of the RNA profiles including mRNA, microRNA and long non-coding RNAs (RNAs) from serum exosomes will be introduced.

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Using QIAGEN Bioinformatics solutions with a publicly available dataset, we have examined the transcriptome of Kupffer cells after uptake of pancreatic tumor-derived exosomes that induce the formation of a liver metastatic niche and analyzed the pathways and biological processes.

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PreAnalytiX, a QIAGEN joined venture with BD, has developed the PAXgene Blood ccfDNA System, consisting of the PAXgene Blood ccfDNA Tube, and the QIAsymphony PAXgene Blood ccfDNA Kit for automated ccfDNA extraction from up to 5 ml of plasma. The slidedeck presents this new technology development and a streamlined workflow for integrated collection, stabilization and purification of circulating cell-free DNA from whole blood.

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Looking for an automated and standardized process to isolate circulating cell-free DNA? This slidedeck features an optimized process for efficient cfDNA enrichment and extraction from plasma samples, and presents a novel automated solution for the extraction of cfDNA using the QIAsymphony SP instrument for liquid biopsy diagnostic applications.

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Frustrated with the poor quality of circulating cell-free DNA (cfDNA) isolated from blood samples? This slidedeck introduces new technical solutions for Ion Torrent, as well as Illumina sequencing platforms that combine optimized cfDNA isolation, highly advanced library construction, unbiased library amplification and integrated data analysis to reliably analyze cfDNA samples with high sensitivity.

This presentation shows how mutations can be best identified from this type of data and how they can be interpreted. Furthermore, potential challenges when analyzing this type of data will be discussed together with relevant strategies.

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Circulating tumor cells (CTCs) are rare and highly heterogeneous. In addition, during the epithelial-mesenchymal transition (EMT) process, CTCs lose their epithelial characteristics and acquire more mesenchymal-like phenotypes, which pose another challenge for CTC detection and characterization. In this slidedeck, Dr. Siegfried Hauch introduces the AdnaTests, which use the Combination of Combinations Principle (COCP) to enable enriching and detecting CTCs in whole blood with high specificity and sensitivity.

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Epithelial ovarian cancer ranks as the second most common type of gynecological malignancy and has poor survival rates. In this presentation, Dr. Sabine Kasimir-Bauer from University of Duisburg-Essen demonstrates that the negative prognostic impact of CTCs and DTCs arises from specific cellular phenotypes and is associated with platinum-based resistance and stem cell-associated proteins.

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