Adeno-associated virus (AAV) has emerged as the primary vector for in vivo gene therapy applications. AAV samples are measured and dosed based on DNA concentration, traditionally assessed through conventional quantitative PCR (qPCR). However, digital PCR methods, which allow for absolute quantification of target sequences and eliminate the need for a standard curve, are replacing qPCR methods due to their improved precision. An accurate AAV titration method is crucial throughout the entire drug development process, including preclinical studies, process development, and clinical dosing.