RNeasy Microarray Tissue Mini Kit
For purification of total RNA from tissues for microarray analysis
The RNeasy Microarray Tissue Mini Kit provides the optimal solution for purifying RNA for microarray analysis. QIAzol Lysis Reagent ensures efficient lysis of even difficult-to-lyse tissues, while trusted RNeasy spin columns allow fast and convenient purification of intact RNA without phenol contamination. The result is highly pure RNA that delivers reliable results in microarray analysis and avoids the need for unnecessary repetition of experiments. For cultured cells, we recommend using the RNeasy Microarray Tissue Mini Kit or RNeasy Plus Mini Kit for RNA purification. The procedure can be fully automated on the QIAcube.
The RNeasy Microarray Tissue Mini Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
RNA purified using the RNeasy Microarray Tissue Mini Kit is highly pure and intact (see figure "Intact RNA"), ensuring reliable results in microarray analysis (see figure "Reliable microarray analysis"). The RNeasy Microarray Tissue procedure is suitable for all tissue types, delivering high yields of RNA from a range of samples, including fibrous and fatty tissues (see table).
The RNeasy Microarray Tissue Mini Kit, with QIAzol Lysis Reagent and RNeasy spin columns, provides the optimal solution for purifying RNA for microarray analysis. Obtaining sufficient yields of RNA, especially from fibrous and fatty tissues, is a challenge. QIAzol Lysis Reagent efficiently lyses all tissue types, delivering optimium starting material for isolation of RNA for microarray analysis. (For fibrous tissue, proteinase K needs to be purchased separately.) Use of RNA containing trace contaminants affects the reverse-transcription step in microarray experiments. RNeasy spin columns efficiently remove phenol and other contaminants, providing highly pure RNA suitable for sensitive applications like microarray analysis.
For the RNeasy Microarray Tissue procedure, tissues are rigorously lysed in phenol-guandine–based QIAzol Lysis Reagent, and RNA is purified from the aqueous phase using an RNeasy spin column (see figure "RNeasy Microarray Tissue procedure"). There is no need to precipitate RNA prior to RNA cleanup, which prevents potential loss of RNA, avoids variable RNA yields, and saves time (see figure "Significant time savings"). For disruption and homogenization of tissues, we recommend TissueRuptor and TissueLyser instruments. Subsequent RNA purification can be automated on the QIAcube.
The RNeasy Microarray Tissue Mini Kit is suitable for isolating RNA for microarray experiments. Purification of RNA from precious samples can be conducted without the risk of failure due to loss of RNA pellets or incomplete removal of contaminants such as phenol. The purified RNA is also ready to use for other applications, such as real-time RT-PCR.
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