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RNeasy Protect Mini Kit

For stabilization and purification of up to 100 µg total RNA from tissues
  • Immediate RNA stabilization and protection
  • Reliable gene expression and gene-profiling data
  • No need for liquid nitrogen, dry ice, or phenol
  • Ready-to-use, high-quality total RNA in minutes
  • No CsCl gradients, no LiCl or ethanol precipitation
The RNeasy Protect Mini Kit includes RNAlater RNA Stabilization Reagent for stabilizing RNA in tissue samples, and RNeasy spin columns for purifying up to 100 µg of high-quality RNA using silica-membrane technology. RNA purification can be automated on the QIAcube. Efficient disruption of tissue samples can be achieved using a TissueRuptor or TissueLyser system.
Cat No./ID: 74124
RNeasy Protect Mini Kit (50)
$429.00
Add To Cart
RNAlater RNA Stabilization Reagent (50 ml), 50 RNeasy Mini Spin Columns, Collection Tubes (1.5 ml and 2 ml), RNase-free Reagents and Buffers
Cat No./ID: 74126
RNeasy Protect Mini Kit (250)
$1,884.00
Add To Cart
RNAlater RNA Stabilization Reagent (250 ml), 250 RNeasy Mini Spin Columns, Collection Tubes (1.5 ml and 2 ml), RNase-free Reagents and Buffers
The RNeasy Protect Mini Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
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RNeasy Protect Mini procedure.

For certain RNA applications that are sensitive to very small amounts of DNA, the residual amounts of DNA remaining can be removed using the RNase-Free DNase Set for convenient on-column DNase treatment during the RNeasy procedure.

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Prevention of degradation of mRNA in tissues.
RNA was isolated from fresh rat kidney samples after 0, 5, 10, 15, 30, and 60 minutes, using either standard procedures (Unstabilized) or RNeasy Protect Kits (Stabilized). The RNA isolated was analyzed by agarose gel electrophoresis and expression of GAPDH was examined using northern blot analysis. M: markers.
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Changes in mRNA levels.
Once a biological sample is harvested, its RNA becomes extremely unstable. Gene induction or down-regulation triggered by sample manipulation must be prevented to enable accurate gene expression analysis.
Performance

The RNA-stabilizing properties of RNAlater RNA Stabilization Reagent prevent gene induction or down-regulation triggered by sample manipulation, and allow purification of high-quality RNA (see figure "Prevention of degradation of mRNA in tissues"). In addition, RNeasy purified RNA from RNAlater stabilized samples remains intact following storage of tissue samples under a wide variety of conditions (see figure "Stable RNA in tissues"). A range of tissue types can be processed with the RNeasy Protect Mini Kit (see table).

Total RNA yields obtained with RNeasy Protect Mini Kit
Mouse tissueAmount (mg) Average yield* (µg)
Brain 10 10
Liver 10 43
Lung 10 12
Spleen 10 45
* Amounts of RNA can vary due to developmental stage, species, and growth conditions of samples.

RNA purified with RNeasy technology has A260/A280 ratios of 1.9–2.1 (measured in 10 mM Tris·Cl, pH 7.5). Since the RNeasy procedure enriches for RNA species >200 nt, RNA yield does not include 5S rRNA, tRNAs, or other low-molecular-weight RNAs.

Principle
The RNeasy Protect system provides a complete RNA protection and isolation solution, from sample harvesting to pure RNA, in one kit. Proven RNeasy silica-membrane technology in spin-column format, combined with the RNA-stabilizing properties of RNAlater RNA Stabilization Reagent, allows purification of high-quality, intact RNA. Once a biological sample is harvested, its RNA becomes extremely unstable (see figure "Changes in mRNA levels"). The innovative, aqueous RNAlater RNA Stabilization Reagent quickly permeates tissues, stabilizing and protecting the RNA expression pattern. Samples can be archived without risk of RNA degradation, even after multiple freeze–thaw cycles. Following stabilization, RNeasy technology simplifies total RNA isolation by combining the stringency of guanidine-isothiocyanate lysis with the speed and purity of silica-membrane purification (see figure "RNeasy Mini spin column").
Procedure

Samples can be stabilized and stored in RNAlater RNA Stabilization Reagent, included in RNeasy Protect Kits. For RNA purification, samples (0.5–30 mg tissue) are first lysed and then homogenized. Ethanol is added to the lysate to provide ideal binding conditions. The lysate is then loaded onto the RNeasy silica membrane (binding capacity up to 100 µg RNA). RNA binds, and all contaminants are efficiently washed away. Pure, concentrated RNA is eluted in 30–100 µl water (see flowchart "RNeasy Protect Mini procedure"). RNA purification can be automated on the QIAcube.

Applications

RNA purified with RNeasy technology is ideal for use in all applications. Downstream applications include:

  • Northern, dot, and slot blotting
  • End-point RT-PCR
  • Quantitative, real-time RT-PCR
  • Array analysis
  • Poly A+ RNA selection
Features
Specifications
Applications PCR, qPCR, real-time RT-PCR, microarray
Elution volume 30–100 µl
Format Spin column
Main sample type Tissue samples
Processing Manual (centrifugation)
Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein RNA
Sample amount 0.5–30 mg
Stabilization Yes
Technology Silica technology
Yield 10 –45 µg

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Kit Handbooks (2)
RNeasy Mini Kit - For purification of total RNA from animal cells, animal tissues, bacteria, and yeast, and for RNA cleanup; RNeasy Protect Mini Kit - For immediate stabilization of RNA in harvested animal tissues and subsequent total RNA purification; RNeasy Plant Mini Kit - For purification of total RNA from plants and filamentous fungi
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RNeasy Mini Kit - 動物細胞、動物組織、バクテリア、酵母からのトータルRNA精製およびRNAクリーンアップ用
RNeasy Protect Mini Kit - 採取した動物組織からのRNAの迅速な安定化と続くトータルRNA精製
RNeasy Plant Mini Kit - 植物および糸状菌類からのトータルRNA精製用
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