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QIAamp 96 Virus QIAcube HT Kit

For automated high-throughput purification of viral RNA and DNA from a variety of samples
  • Simple and reliable automated processing for cost and time savings
  • Suitable for many samples, including blood, tissues, swabs and body fluids
  • Consistent, high yields
  • Efficient removal of inhibitors and contaminants
  • Purified nucleic acids ready for analysis by real-time PCR or RT-PCR

The QIAamp 96 Virus QIAcube HT Kit enables simple, automated purification of viral RNA and DNA on the QIAcube HT system. Using proven QIAamp silica-membrane technology in a convenient 96-well format, contaminants and inhibitors are removed to yield high-quality nucleic acids that are ready for downstream analysis.

Cat No./ID: 57731
QIAamp 96 Virus QIAcube HT Kit (5)
$1,711.00
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For 480 preps: QIAamp 96 plates, QIAGEN Proteinase K, carrier RNA, buffers
Cat No./ID: 950067
QIAcube HT Plasticware
$360.00
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Plasticware for 480 typical preps on QIAcube HT:
5 S-Blocks, 5 Elution Microtubes RS (EMTR), 120 x 8-Well Strip Caps for EMTR, 9 x 96 Filter-Tips OnCor C, TapePad
The QIAamp 96 Virus QIAcube HT Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
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Reliable automated purification.
Viral nucleic acids were purified from 200 µl plasma samples spiked with 10,000, 1000 and 100 IU/ml of a typical DNA or RNA virus. Sample processing was automated using either QIAcube HT with the QIAamp 96 Virus QIAcube HT Kit and protocol, QIAxtractor with the VX Protocol, or QIAcube with the QIAamp MinElute Virus Spin Kit. Viral nucleic acids were detected using in-house PCR and RT-PCR assays, with 20 µl eluate per reaction on the Rotor-Gene Q. Results show that QIAcube HT with the QIAamp 96 Virus QIAcube HT Kit performs as well as or better than the other methods.
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Reliable purification from a range of sample types.
Various sample types were spiked with 20,000 IU of a typical DNA virus. Viral DNA was purified from 200 µl of each sample lysate using the QIAcube HT with the QIAamp 96 Virus QIAcube HT Kit and protocol. Purified viral DNA was detected using an in-house PCR assay with 20 µl each eluate per reaction.
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High sensitivity of purified viral nucleic acids in PCR and RT-PCR.
Nucleic acids were purified from serial dilutions of plasma samples spiked with a typical RNA or DNA virus. Samples were processed using QIAcube HT with the QIAamp 96 Virus QIAcube HT Kit and protocol and were analyzed using in-house PCR and RT-PCR assays. The percentage of positive samples at low virus titers is shown. The resulting 95% probit values were 316.84 IU/ml for the RNA virus and 18.54 IU/ml for the DNA virus.
Performance
The QIAamp 96 Virus QIAcube HT Kit enables automated purification of viral RNA and DNA from a broad range of sample types including fresh or frozen tissues, blood and other body fluids. The procedure yields high-quality viral nucleic acids that perform well in downstream PCR and RT-PCR analyses (see figure Reliable purification from a range of sample types).

QIAcube HT with the dedicated QIAamp 96 Virus QIAcube HT Kit lets users increase sample purification throughput without having to compromise on quality or reliability. The procedure provides high yields of pure RNA or DNA that perform well in downstream analyses, similar to other QIAGEN DNA purification solutions (see figures Reliable automated purification and High sensitivity of viral nucleic acids in PCR and RT-PCR).

Principle
The QIAamp 96 Virus QIAcube HT Kit combines the selective binding properties of a silica-based membrane with a high-throughput 96-well format, and is designed for fully automated, simultaneous processing of 24–96 samples on the QIAcube HT instrument.
 
Kit specifications
Specification Description
Number of samples 24–96 samples (to be processed in increments of 8)
Sample input volume Blood and other body fluids: up to 200 μl (for sample volumes less than 200 μl, add PBS) 
Tissues: up to 20 mg tissue
Elution volume 100 μl
Duration 96 samples in approximately 145 minutes
24 samples in approximately 90 minutes
Procedure
The QIAamp 96 Virus QIAcube HT procedure is fast and simple with the QIAcube HT instrument. Samples are lysed under highly denaturing conditions at room temperature in the presence of QIAGEN proteinase K and Buffer ACL, which together ensure the inactivation of nucleases. Adding Buffer ACB adjusts the binding conditions for the co-purification of DNA and RNA. The lysate is then transferred to a QIAamp 96 plate. During vacuum, nucleic acids are adsorbed onto the silica membranes while contaminants pass through. Three efficient wash steps remove the remaining contaminants and enzyme inhibitors, and nucleic acids are eluted in Buffer AVE. Some samples may require a pretreatment.
Applications
The high-quality nucleic acids are ready to use in all downstream applications, including sensitive detection assays using quantitative, real-time PCR or RT-PCR.

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Brochures & Guides (1)
For fast and reliable automated 96-well nucleic acid purification
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Protocol Files (4)
Procedure for installing Q Protocols for QIAcube HT Kits on the QIAcube HT and the QIAxtractor. For use with QIAcube HT Operating Software 4.17; not for use with QIAcube HT Prep Manager Software.
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Q Protocol notes for purification of viral nucleic acids from human samples on the QIAcube HT using the QIAamp 96 Virus QIAcube HT Kit; use with the QIAamp 96 Virus QIAcube HT V1.qsp run file. For use with QIAcube HT Operating Software 4.17; not for use with QIAcube HT Prep Manager Software.
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Installation file (.msi) for installing the Q Protocol run file (.qsp) in the QIAcube HT software. For use with QIAcube HT Operating Software 4.17; not for use with QIAcube HT Prep Manager Software.
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These Q Protocols and installation files can only be used with QIAcube HT Operating software version 4.17 and are not compatible with the new QIAcube HT Prep Manager software.

QIAcube HT Prep Manager Software already includes all standard protocols. Additional protocols and kit configurations can be easily installed. Check for available updates under the "Operating Software" section of the "Product Resources" tab.
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Instrument User Manuals (2)
Instrument user manual for automated mid- to high-throughput nucleic acid purification in 96-well format using silica membrane technology; for use with QIAcube HT Operating Software 4.17; not for use with QIAcube HT Prep Manager Software
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Instrument user manual for automated mid- to high-throughput nucleic acid purification in 96-well format using silica membrane technology; for use with QIAcube HT Prep Manager Software.
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Quick-Start Protocols (3)
For use with QIAcube HT Operating Software 4.17; not for use with QIAcube HT Prep Manager Software
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For use with QIAcube HT Operating Software 4.17; not for use with QIAcube HT Prep Manager Software
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For use with QIAcube HT Prep Manager Software; not compatible with QIAcube HT Operating Software 4.17
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Kit Handbooks (2)
For use with QIAcube HT Operating Software 4.17; not compatible with QIAcube HT Prep Manager Software
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For use with QIAcube HT Prep Manager Software; not compatible with QIAcube HT Operating Software 4.17
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