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PAXgene Blood RNA Tubes (IVD)

For collection of whole blood samples for molecular diagnostic testing

  • Component of the FDA-cleared, CE-marked PAXgene Blood RNA System
  • Immediate stabilization of intracellular RNA and miRNA at collection
  • Specimen transport and storage at room and refrigerated temperatures
  • Frozen specimen storage for up to 8 years
  • Standardized preanalytic processing of samples

 

The PAXgene Blood RNA Tube (IVD) is a component of the PAXgene Blood RNA System, which additionally includes the PAXgene Blood RNA Kit (IVD) for nucleic acid purification. The PAXgene Blood RNA System is intended for the collection, transport and storage of blood and the stabilization of intracellular RNA in a closed tube with subsequent isolation and purification of intracellular RNA for RT-PCR used in molecular diagnostic testing.

Cat No./ID: 762125
PAXgene Blood RNA Tubes (100)
100 PAXgene Blood RNA Tubes; available only via www.PreAnalytiX.com (cat. no. 762165)
PAXgene Blood RNA Tubes (IVD) are intended for in vitro diagnostics only when used with the PAXgene Blood RNA Kit (IVD).

For up-to-date licensing information and product-specific disclaimers, see the respective QIAGEN handbook or PreAnalytix product circular.



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Repeatability and reproducibility of RNA yield for different operators and PAXgene Blood RNA Kit lots
Blood samples from 30 different donors were collected in PAXgene Blood RNA Tubes (12 tubes per donor, 360 tubes in total). The contents of the tubes from 3 donors were pooled and subsequently re-aliquoted into 36 samples. These 36 samples per 3-donor-pool were manually processed by 3 different operators. Each operator used 3 different PAXgene Blood RNA Kit lots for the extraction and processed quadruplicate samples from each of the 10 donor pools. [A] RNA yield and standard deviation for every operator-lot combination. Quadruplicate blood samples from 10 donor pools were processed by 3 different operators (A, B, C) with each of 3 kit lots (1, 2, 3). The mean yields (columns) and standard deviations (error bars) per quadruplicate sample from the same donor pool for different operator and different kit lot are presented. [B] CV of RNA yield per donor pool for all operator-lot combinations (A, B, C; 1, 2, 3) as calculated from the mean yield and standard deviation of the yield shown in part A.
Performance

The patented additive in PAXgene Blood RNA Tubes (IVD) protects RNA molecules from degradation by RNases and other enzymes, and triggers the complete lysis of all cells in the sample to minimize ex vivo changes in gene expression due to gene induction and downregulation. The result is immediate and long-term stabilization of RNA in specimens, even after being stored for up to 3 days at room temperature (see figures "RNA stability at 18–25°C: FOS" and "RNA stability at 18–25°C: IL1B") or up to 5 days refrigerated (see figures "RNA stability at 2–8°C: FOS" and "RNA stability at 2–8°C: IL1B"). Currently available data show stabilization of intracellular RNA for up to 96 months at  –20°C or –70°C.

Performance characteristics of the PAXgene Blood RNA System have been established with FOS and IL1B gene transcripts. The user is responsible for establishing appropriate PAXgene Blood RNA System performance characteristics for other target transcripts.

Principle

The collection of whole blood is the first step in many molecular assays used to study intracellular RNA. A major challenge in this type of testing is the instability of intracellular RNA, which rapidly degrades within hours after blood collection. Furthermore, certain transcript levels change after blood collection due to ex vivo gene regulation, such as induction and repression. Both RNA degradation and gene regulation can lead to an under- or overestimation of in vivo gene expression profiles.

The PAXgene Blood RNA Tube contains an additive that stabilizes in vivo gene expression profiles by reducing post-collection RNA degradation and minimizing gene induction and repression. When used in conjunction with one of the PAXgene Blood RNA kits, the PAXgene Blood RNA Tube (IVD) provides pure RNA for accurate detection and quantification of gene expression.

Procedure
Whole blood is collected directly into the PAXgene Blood RNA Tube (IVD) using standard phlebotomy practice and equipment. The RNA in the specimen is immediately stabilized for storage at room temperature, refrigerated or frozen. RNA must be purified using the PAXgene Blood RNA Kit (IVD) for molecular diagnostics applications. PAXgene Blood RNA Tubes (IVD) can be combined with other PAXgene Blood RNA kits for Research Use Only (RUO) applications.

The ease of collection, stabilization of RNA at different temperatures and elimination of additional processing steps (e.g., centrifugation, storage on ice) standardizes the workflow from collection to purification, reducing variability that can be introduced by different operators (see figure "Repeatability and reproducibility of RNA yield for different operators and PAXgene Blood RNA Kit lots"). 

 

Applications

Used together, the PAXgene Blood RNA Tube (IVD) and the PAXgene Blood RNA Kit (IVD) constitute the FDA-cleared, CE-marked PAXgene Blood RNA System to enable the purification of intracellular RNA from whole blood for applications in molecular diagnostic testing, such as real-time RT-PCR analysis of specific transcripts.

Features
Specifications
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Quantity 100/box
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Brochures & Guides (2)
For collection, transport, and storage of whole blood and stabilization and purification of intracellular RNA

 


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For molecular diagnostic testing

 


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Kit Handbooks (1)
For collection of whole blood samples for molecular diagnostic testing
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Application Notes (3)
Comparison of miRNA recovery from the PAXgene Blood miRNA System and the Tempus Blood RNA Tube processed with the Tempus Spin RNA Isolation Kit

 


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In situ stability of RNA in blood specimens stored for 8 years (96 months) at –20°C and –70°C in PAXgene Blood RNA Tubes

 


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Typical total RNA yields from PAXgene Blood RNA Tubes processed with the PAXgene Blood RNA Kit
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