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PAXgene Tissue Containers

For fixation and stabilization of human tissue specimens (max. 4 x 15 x 15 mm in size)
  • Preservation of both morphology and biomolecules
  • Fixation and paraffin embedding without formalin
  • Improved molecular results from fixed tissues
  • Tissue can be stored and archived for later processing
  • RNA, miRNA, DNA, and/or proteins from one sample

PAXgene Tissue Containers are dual-cavity containers prefilled with 2 reagents. PAXgene Tissue FIX rapidly penetrates and fixes the tissue, preserving tissue morphology. Fixation is comparable to formalin fixation, but without the destructive nucleic acid and protein crosslinking and degradation. After fixation, the tissue is transferred to PAXgene Tissue STABILIZER in the same container. Nucleic acids, proteins, and morphology of the sample are stable up to 7 days at room temperature and for longer periods at 2–8°C, or even at –20°C and –80°C. Stabilized samples can be embedded in paraffin for histological studies. PAXgene Tissue Kits and supplementary protocols provide efficient subsequent purification of RNA, miRNA, DNA, and/or proteins from the same sample.

Cat No./ID: 765112
PAXgene Tissue Container (10)
$165.00
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For collection, fixation, and stabilization of 10 samples: 10 Prefilled Reagent Containers, containing PAXgene Tissue FIX and PAXgene Tissue STABILIZER
For Research Use Only. Not for use in diagnostics procedures. No claim or representation is intended to provide information for the diagnosis, prevention, or treatment of a disease.
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Preservation of tissue morphology.
Kidney tissue was fixed and stabilized with the PAXgene Tissue Container, and 4 μm sections were stained with hematoxylin and eosin (HE). [A] Overview of sections taken from mirrored samples. [B] Tissue fixed with PAXgene Tissue FIX (viewed at 630x magnification). [C] Tissue fixed with neutral buffered formalin  (viewed at 630x magnification).
Performance
The fixation and stabilization method preserves tissue morphology and the integrity of nucleic acids without destructive crosslinking and degradation found in formalin-fixed tissues (see figure "Preservation of tissue morphology").

When  tissue is stored in PAXgene Tissue STABILIZER, nucleic acids, proteins and morphology of the tissue sample are stable for a minimum of 7 days at room temperature (15–25°C) or for a minimum of 4 weeks at 2–8°C, depending on the type of tissue. Tissue samples can even be stored in the PAXgene Tissue STABILIZER for longer periods at –20°C (–15°C to –30°C) or –80°C (–65°C to –90°C) without negative effects on the morphology of the tissue or the integrity of the nucleic acids.

Specifications for fixation and storage conditions in PAXgene Tissue FIX and PAXgene Tissue STABILIZER were determined using animal tissues.
Principle
The methods for tissue fixation currently used in traditional histology are of limited use for molecular analysis. Fixatives that contain formaldehyde crosslink biomolecules and modify nucleic acids and proteins. Such crosslinks lead to nucleic acid degradation during tissue fixation, storage, and processing. Since they cannot be removed completely, the resulting chemical modifications can cause inhibition in downstream applications, such as quantitative PCR or RT-PCR. To enable both molecular and traditional pathology testing from the same specimen, a method is needed to stabilize molecular content and preserve morphology.

To meet this need, PreAnalytiX has developed the PAXgene Tissue System. The system consists of a fixation reagent (PAXgene Tissue FIX), a stabilization reagent (PAXgene Tissue STABILIZER), prefilled containers for tissue collection, storage, and transportation, and kits for purification of RNA, DNA, or total RNA, including miRNA. In addition, supplementary protocols for protein purification and other applications are available in the 'Resources' section of this page or at www.preanalytix.com.

PAXgene Tissue reagents in prefilled containers and PAXgene Tissue Kits provide a complete preanalytical solution for collection, fixation, and stabilization of tissue, and purification of high-quality nucleic acids for molecular research analysis.

 

Procedure
PAXgene Tissue Containers are dual-chamber containers prefilled with 2 reagents. Chamber 1 contains PAXgene Tissue FIX and chamber 2 contains PAXgene Tissue STABILIZER. For fixation, tissue samples with a maximum size of 4 x 15 x 15 mm must be placed into a standard tissue cassette.

Fixation is performed in chamber 1 for 2–24 hours. PAXgene Tissue FIX rapidly penetrates and fixes the tissue. After fixation, the tissue cassette is removed from chamber 1 and transferred to PAXgene Tissue STABILIZER in the second chamber of the same container. Stabilized samples can be embedded in paraffin for histological studies. Nucleic acids and proteins can be isolated from the stabilized samples before or after embedding in paraffin. See the PAXgene Tissue Kit Handbooks for information about DNA, RNA, or miRNA, and the PAXgene Tissue supplementary protocols under 'Resources' section of this page or at www.preanalytix.com for protein purification and other applications.

Applications
Sections of PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue can be used for histological studies or extraction of nucleic acids or proteins. Purification of RNA, total RNA, including miRNA, or DNA from PAXgene Tissue fixed and stabilized tissue samples requires the use of one of the PAXgene Tissue Kits for RNA, miRNA, or DNA. Purification of protein requires the Qproteome FFPE Kit.

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Brochures & Guides (6)
For isolation and purification of genomic DNA from tissue samples stabilized using the PAXgene Tissue System 

 


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For isolation and purification of total RNA, including miRNA, from tissue samples fixed and stabilized using the PAXgene Tissue System

 


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For extraction of full-length proteins from PFPE tissue using the Qproteome FFPE Tissue Kit

 


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For isolation and purification of intracellular RNA from tissue samples stabilized using the PAXgene Tissue System

 


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Moving toward excellence and standardization in tissue collection and fixation

 


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FAQs (34)
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Kit Handbooks (1)
For fixation and stabilization of tissue specimens
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Technical Information (10)

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Scientific Posters (15)
Long et al., ADAPT 2012

 


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Hesse et al., AACR-NCI-EORTC 2011

 


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Groelz et al., AACR 2010

 


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Groelz et al., AMP 2010 

 


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Groelz et al., AMP 2008 

 


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Groelz et al., AMP 2009 

 


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Groelz et al., ISBER 2012

 


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Groelz et al., BRN Symposium 2012 

 


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Groelz et al., ECP 2012

 


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Groelz et al., AMP 2009

 


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Groelz et al., 3rd Annual Oncology Biomarkers 2011

 


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Groelz et al., GTEx Meeting 2014

 


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Groelz et al., AMP 2014

 


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Groelz et al., BRN Symposium 2011 

 


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Groelz et al., ECP 2014

 


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Supplementary Protocols (16)

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