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Qproteome Sialic Glycoprotein Kit

For specific enrichment of glycoproteins with sialic-acid-rich glycan moieties
  • Specific separation of glycoproteins according to their glycan moieties
  • Glycoprotein profiling in cells grown under different conditions or in disease states
  • Comprehensive glycoprotein characterization 
 
The Qproteome Sialic Glycoprotein Kit contains three different lectin-resin-filled spin columns, buffers, and reagents for the isolation of glycoproteins with sialic-acid-rich glycan moieties from 6 cell lysate or serum samples.

Cat No./ID: 37561
Qproteome Sialic Glycoprotein Kit
For 6 sialic acid glycoprotein preps: WGA, SNA, and MAL Lectin Spin Columns (2 each); Buffers; Detergent Solution; Protease Inhibitor Solution; Collection Tubes (6 x 2 ml)
The Qproteome Sialic Glycoprotein Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
Performance
The Qproteome Sialic Glycoprotein Kit enables highly specific enrichment of glycoproteins with sialic-acid-rich glycan moieties (see figure Highly specific glycoprotein fractionation). The selection of lectin columns allow accurate characterization of glycoproteins.

 

Principle
Proteins with sialic acid-rich glycan moieties bind to lectins immobilized on the resin in the spin columns. After washing, proteins are eluted by addition of an elution buffer containing sugars which compete for lectin binding sites with the bound proteins.
Procedure
The sample is diluted and applied to the spin column (see figure Glycoprotein fractionation procedure). After a short incubation, proteins that have not bound to the lectin are removed by centrifugation. Bound proteins are eluted by application of elution buffer and centrifugation.
Applications
Glycosylation plays a vital role in a wide range of cellular processes such as cell adhesion and signaling, stabilization of protein structure and function, protein trafficking and sorting, and oncogenesis. Several diseases (e.g., rheumatoid arthritis, muscular dystrophy) may be caused by a defect in protein glycosylation. Qproteome Glycoprotein Fractionation Kits offer highly specific separation of glycoproteins according to the structure of their glycan moieties, and permit profiling of glycoproteins in cells grown under different conditions.
Features
Specifications
Applications SDS-PAGE, mass spectrometry
Binding capacity/yield 30 µg - 150 µg
For glycoproteins: which type of glycoproteins Sialic-acid-rich glycoproteins
Fractions isolated One fraction
Protein with post-translational modification Glycosylation
Sample size 50 µl or 1 x 10e7 cells
Start material Serum, cell cultures
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