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QIAprep 96 Turbo Kits

For purification of up to 20 µg molecular biology grade plasmid DNA per well

  • Streamlined purification
  • Ready-to-use plasmid DNA within 70 minutes
  • Reproducible yields of molecular biology grade plasmid DNA

The QIAprep 96 Turbo Kits provide QIAprep 96 and TurboFilter 96 plates for purification of up to 20 µg molecular biology grade plasmid DNA per well.
For optimal results it is recommended to use this product together with QIAvac 96.

Cat No./ID: 120012
QIAprep 96 Plates (24)
$2,772.00
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QIAprep 96 Plates (24)
Cat No./ID: 120025
TurboFilter 96 Plates (96)
$9,981.00
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TurboFilter 96 Plates (96)
Cat No./ID: 27173
QIAprep 96 Turbo Core Kit (24)
$6,109.00
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Custom kit configuration. Please inquire for details.
Cat No./ID: 27191
QIAprep 96 Turbo Miniprep Kit (4)
$1,291.00
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For 4 x 96 high-purity plasmid minipreps, 4 each: TurboFilter 96 and QIAprep 96 Plates; Flat-Bottom Blocks and Lids, Reagents, Buffers, Collection Microtubes (1.2 ml), Caps
Cat No./ID: 27193
QIAprep 96 Turbo Miniprep Kit (24)
$7,299.00
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For 24 x 96 high-purity plasmid minipreps, 24 each: TurboFilter 96 and QIAprep 96 Plates; Flat-Bottom Blocks and Lids, Reagents, Buffers, Collection Microtubes (1.2 ml), Caps
Cat No./ID: 962141
QIAprep 96 Turbo BioRobot Kit (4)
$1,294.00
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For 4 x 96 high-purity plasmid minipreps, 4 each: TurboFilter 96 and QIAprep 96 Plates; Flat-Bottom Blocks and Lids, Reagents, Buffers, Collection Microtubes (1.2 ml) and Caps, 96-Well Microplates RB and Lids, Tape Pads

QIAprep 96 Turbo Kits are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.


 


Performance

The QIAprep 96 Turbo Kits are designed for isolation of up to 20 µg pure plasmid or cosmid DNA for use in routine molecular biology applications, such as sequencing and cloning (see figure "High-quality DNA for automated sequencing").

In addition to plasmid purification from Escherichia coli, QIAprep 96 Turbo Kits can be used to purify plasmid DNA from Saccharomyces cerevisiae, Bacillus subtilis, and Agrobacterium tumefaciens. Contact QIAGEN Technical Services or your local distributor for protocols for these applications.

Principle

QIAprep 96 plates (see figure "QIAvac 96 with 96-well plate") contain a unique silica membrane that binds up to 20 µg DNA in the presence of a high concentration of chaotropic salt, and allows elution in a small volume of low-salt buffer. QIAprep membrane technology eliminates time consuming phenol–chloroform extraction and alcohol precipitation, as well as the problems and inconvenience associated with loose resins and slurries. Pure plasmid DNA eluted from QIAprep 96 plates is immediately ready to use — there is no need to precipitate, concentrate, or desalt.

TurboFilter 96 plates are specially designed to allow fast clearing of bacterial lysates generated under high concentrations of chaotropic salts. Crude bacterial lysates are vacuum filtered through the TurboFilter 96 plate, eliminating the need for centrifugation. Particle-free lysates flow directly into the wells of the QIAprep 96 plate, ready for purification and elution. TurboFilter technology streamlines QIAprep plasmid purification and allows the procedure to be fully automated on the BioRobot 3000, the BioRobot 8000, or the BioRobot Universal System.

Specifications
Features QIAprep 96 Turbo Miniprep Kit QIAprep 96 Turbo BioRobot Kit
Applications Fluorescent and radioactive sequencing, cloning, etc. Fluorescent and radioactive sequencing, cloning, etc.
Culture volume/starting material 1–10 ml culture volume 1–10 ml culture volume
Plasmid type High-copy, low-copy, cosmid DNA High-copy, low-copy, cosmid DNA
Processing Manual (vacuum) Manual (vacuum)
Samples per run; throughtput 48–96 samples per run 48–96 samples per run
Technology Silica technology Silica technology
Time per run <45 min/plate <70 minutes
Yield <20 µg <20 µg

Procedure

Plasmid purification using QIAprep 96 Turbo Kits follow a simple bind–wash–elute procedure (see flowchart "The QIAprep procedure"). First, bacterial cultures are lysed and the lysates are cleared by filtration through TurboFilter modules. The cleared lysates are then applied to the QIAprep 96 plate where plasmid DNA adsorbs to the silica membrane. Impurities are washed away and pure DNA is eluted in a small volume of elution buffer or water.

The QIAprep 96 Turbo procedure is performed entirely in 96-well format, reducing sample handling to a minimum, allowing 96 parallel minipreps to be processed in 45–70 minutes. Bacterial cultures can be grown and lysed in the 96-well blocks provided with the kit. The QIAvac 96 manifold is designed for in-line filtration in 96-well format, so that cleared lysates from the TurboFilter 96 plate flow directly into the wells of the QIAprep 96 plate, providing high-throughput of 96 samples in parallel.The QIAprep 96 Turbo BioRobot Kit is a special kit format optimized on the BioRobot 8000 (no longer available) or the BioRobot Universal System. The kit provides all components required for automated preparation of 96 high-purity plasmid minipreps.

Applications

QIAprep 96 Turbo Kits provide reproducible yields of pure DNA suitable for use in many applications, including:

Screening
PCR 
Sequencing
Restriction digestion
Ligation and transformation

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