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PAXgene Tissue FIX Container (50 ml)

For fixation and stabilization of tissues specimens
  • Preservation of both morphology and biomolecules
  • Fixation and paraffin embedding without formalin
  • Improved molecular results from fixed tissues
  • Tissue can be stored and archived for later processing
  • RNA, miRNA, DNA, and/or proteins from one sample
The PAXgene Tissue FIX Container (50 ml) enables fixation and stabilization of tissue specimens (up to 4 samples with a maximum size of 4 x 15 x 15 mm placed into 4 standard tissue cassettes, or a single tissue sample with a maximum size of 20 x 20 x 20 mm). PAXgene Tissue FIX Containers (50 ml) are single-chamber containers prefilled with 50 ml of PAXgene Tissue FIX. PAXgene Tissue FIX rapidly penetrates and fixes the tissue, preserving tissue morphology. Fixation is comparable to formalin fixation, but without the destructive nucleic acid crosslinking and degradation. After fixation, PAXgene Tissue FIX is removed and replaced by PAXgene Tissue STABILIZER within the same container. Nucleic acids, proteins, and morphology of the sample are stable up to 7 days at room temperature, and for longer periods at 2–8°C, or even at –20°C and –80°C. Stabilized samples can be embedded in paraffin for histological studies. PAXgene Tissue Kits and supplementary protocols provide efficient subsequent purification of RNA, miRNA, DNA, and/or proteins from the same sample.

PAXgene Tissue FIX Container (50 ml) is only to be used in conjunction with PAXgene Tissue STABILIZER.
Cat No./ID: 765312
PAXgene Tissue FIX Container (50 ml)
$212.00
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For fixation and stabilization of tissue specimen: 10 prefilled Reagent Containers containing 50 ml of PAXgene Tissue FIX
For Research Use Only. Not for use in diagnostics procedures. No claim or representation is intended to provide information for the diagnosis, prevention, or treatment of a disease.
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Preservation of tissue morphology.
Tissue was fixed and stabilized with the PAXgene Tissue FIX Container (50 ml); 4 µm sections of PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue were stained with hematoxylin and eosin (HE). [A] Rat liver, [B] rat kidney, [C] rat intestine. Overview at 40x, details at 400x magnification.
Performance
The fixation and stabilization method preserves tissue morphology and the integrity of nucleic acids without destructive crosslinking and degradation found in formalin-fixed tissues (see figure "Preservation of tissue morphology").

When fixed tissue is stored in PAXgene Tissue STABILIZER, the nucleic acids, proteins, and morphology of the tissue sample are stable for up to 7 days at room temperature (15–25°C) or for up to 4 weeks at 2–8°C. Tissue samples can even be stored in the PAXgene Tissue STABILIZER for longer periods at –20°C (–15°C to –30°C) or –80°C (–65°C to –90°C) without negative effects on the morphology of the tissue or the integrity of the nucleic acids.

Specifications for fixation and storage conditions in PAXgene Tissue FIX and PAXgene Tissue STABILIZER were determined using animal tissues.


Principle
The methods for tissue fixation currently used in traditional histology are of limited use for molecular analysis. Fixatives that contain formaldehyde crosslink biomolecules and modify nucleic acids and proteins. Such crosslinks lead to nucleic acid degradation during tissue fixation, storage, and processing. Since they cannot be removed completely, the resulting chemical modifications can cause inhibition in downstream applications, such as quantitative PCR or RT-PCR. To enable both molecular and traditional pathology testing from the same specimen, a method is needed to stabilize molecular content and preserve morphology.

To meet this need, PreAnalytiX has developed the PAXgene Tissue System. The system consists of a fixation reagent (PAXgene Tissue FIX), a stabilization reagent (PAXgene Tissue STABILIZER), prefilled containers for tissue collection, storage, and transportation, and kits for purification of RNA, DNA, or total RNA, including miRNA. In addition, supplementary protocols for protein purification and other applications are available in the 'Resources' section of this page or at www.preanalytix.com.

PAXgene Tissue reagents in prefilled containers and PAXgene Tissue Kits provide a complete preanalytical solution for collection, fixation, and stabilization of tissue, and purification of high-quality nucleic acids for molecular research analysis.

 

Procedure
PAXgene Tissue FIX Containers (50 ml) are single-chamber containers prefilled with 50 ml of PAXgene Tissue FIX. PAXgene Tissue FIX Containers (50 ml) can accommodate four standard tissue cassettes (not provided), which can hold tissue samples with a maximum size of 4 x 15 x 15 mm. PAXgene Tissue FIX Containers (50 ml) also offer the possibility for direct fixation of larger tissue samples with a maximum size of 20 x 20 x 20 mm.

PAXgene Tissue FIX rapidly penetrates and fixes the tissue. After fixation for 2 to 48 hours, depending on tissue size, PAXgene Tissue FIX is removed and replaced by PAXgene Tissue STABILIZER within the same container. Stabilized samples can be embedded in paraffin for histological studies. Nucleic acids and proteins can be isolated from the stabilized samples before or after embedding in paraffin. See the PAXgene Tissue Kit Handbooks for information about DNA, RNA, or miRNA, and the PAXgene Tissue supplementary protocols in the 'Resources' section of this page or at www.preanalytix.com for protein purification and other applications.
Applications
Sections of PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue can be used for histological studies or extraction of nucleic acids or proteins. Purification of RNA, total RNA, including miRNA, or DNA from PAXgene Tissue fixed and stabilized tissue samples requires the use of one of the PAXgene Tissue Kits for RNA, miRNA, or DNA. Purification of protein requires the Qproteome FFPE Kit.

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Brochures & Guides (6)
For isolation and purification of genomic DNA from tissue samples stabilized using the PAXgene Tissue System 

 


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For isolation and purification of total RNA, including miRNA, from tissue samples fixed and stabilized using the PAXgene Tissue System

 


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For extraction of full-length proteins from PFPE tissue using the Qproteome FFPE Tissue Kit

 


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For isolation and purification of intracellular RNA from tissue samples stabilized using the PAXgene Tissue System

 


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Moving toward excellence and standardization in tissue collection and fixation

 


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FAQs (34)
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Kit Handbooks (2)
For collection, fixation, and stabilization of multiple small tissue samples or a single large tissue sample

 


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For stabilization of tissue specimens fixed in PAXgene Tissue FIX Container

 


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Scientific Posters (15)
Long et al., ADAPT 2012

 


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Hesse et al., AACR-NCI-EORTC 2011

 


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Groelz et al., AACR 2010

 


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Groelz et al., AMP 2010 

 


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Groelz et al., AMP 2008 

 


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Groelz et al., AMP 2009 

 


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Groelz et al., ISBER 2012

 


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Groelz et al., BRN Symposium 2012 

 


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Groelz et al., ECP 2012

 


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Groelz et al., AMP 2009

 


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Groelz et al., 3rd Annual Oncology Biomarkers 2011

 


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Groelz et al., GTEx Meeting 2014

 


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Groelz et al., AMP 2014

 


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Groelz et al., BRN Symposium 2011 

 


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Groelz et al., ECP 2014

 


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Supplementary Protocols (16)

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Technical Information (10)

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