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MinElute Reaction Cleanup Kit

For cleanup of up to 5 μg DNA (70 bp to 4 kb) from enzymatic reactions
  • Very small elution volumes
  • Fast procedure and easy handling
  • High, reproducible recoveries
  • Gel loading dye for convenient sample analysis

The MinElute Reaction Cleanup Kit provides spin columns, buffers, and collection tubes for silica membrane-based purification of DNA 70 bp – 4 kb in size from enzymatic reactions. The spin columns are designed to allow elution in very small volumes (as little as 10 μl), delivering highly concentrated DNA in high yields. An integrated pH indicator allows easy determination of the optimal pH for DNA binding to the spin column. The procedure can be fully automated on the QIAcube. DNA fragments purified with the MinElute system are ready for direct use in all applications, including sequencing, microarray analysis, ligation and transformation, restriction digestion, labeling, microinjection, PCR, and in vitro transcription.
For optimal results it is recommended to use this product together with QIAvac 24 Plus.

Cat No./ID: 28204
MinElute Reaction Cleanup Kit (50)
$142.00
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50 MinElute Spin Columns, Buffers, Collection Tubes (2&nbsp:ml)
Cat No./ID: 28206
MinElute Reaction Cleanup Kit (250)
$650.00
Add To Cart
250 MinElute Spin Columns, Buffers, Collection Tubes (2 ml)
The MinElute Reaction Cleanup Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
Performance

The MinElute Reaction Cleanup Kit ensures cleanup of up to 5 μg DNA (70 bp to 4 kb) from enzymatic reactions, delivering high yields of DNA suitable for a range of applications. The kit provides spin columns for cleanup of enzymatic reactions. Using a microcentrifuge or vacuum manifold, high concentration of DNA fragment (70 bp – 4 kb) is quickly achieved. (DNA fragments larger than 4 kb should be purified using the QIAquick System.)

Examples of enzymes that are completely removed by the MinElute Reaction Cleanup Kit
ProteinMolecular weight per enzyme subunit (kDa)
DNA Polymerase I 109
Klenow fragment 62
Calf intestinal alkaline phosphatase 69
T4 DNA ligase 55
T4 Polynucleotide kinase 35
Terminal transferase 32
DNase I 31
Restriction enzymes Varies
Principle

MinElute Kits contain a silica membrane assembly for binding of DNA in high-salt buffer and elution with low-salt buffer or water. The purification procedure removes primers, nucleotides, enzymes, mineral oil, salts, agarose, ethidium bromide, and other impurities from DNA samples. Silica-membrane technology eliminates the problems and inconvenience associated with loose resins and slurries. Specialized binding buffers are optimized for specific applications and promote selective adsorption of DNA molecules within particular size ranges.

Gel loading dye

To enable faster and more convenient sample processing and analysis, gel loading dye is provided. GelPilot Loading Dye contains three tracking dyes (xylene cyanol, bromophenol blue, and orange G) to facilitate the optimization of agarose gel run time and prevent smaller DNA fragments migrating too far (see figure "GelPilot Loading Dye").

Procedure

The MinElute system uses a simple bind-wash-elute procedure (see flowchart "MinElute procedure"). Binding buffer is added directly to the enzymatic reaction, and the mixture is applied to the MinElute spin column. The binding buffer contains a pH indicator, allowing easy determination of the optimal pH for DNA binding (see figure "pH Indicator Dye"). Nucleic acids adsorb to the silica membrane in the high-salt conditions provided by the buffer. Impurities are washed away and pure DNA is eluted with a small volume of low-salt buffer provided or water, ready to use in subsequent applications.

Handling

MinElute spin columns are designed to provide two convenient handling options (see flowchart "MinElute procedure"). The spin columns fit into a conventional table-top microcentrifuge or onto any vacuum manifold with luer connectors, such as QIAvac 24 Plus or QIAvac 6S with QIAvac Luer Adapters. The MinElute Reaction Cleanup Kit, in addition to other QIAGEN spin-column-based kits, can be fully automated on the QIAcube, enabling increased productivity and standardization of results (see figures "Spin column handling options A, B, C, D, and E").

Applications

DNA fragments purified with the MinElute System are ready for direct use in all applications, including:

Sequencing
Microarray analysis
Ligation and transformation
Restriction digestion
Labeling
Features
Specifications
Binding capacity 5 µg
Elution volume 10 µl
Format Tube
Fragment size 70 bp – 4 kb
Processing Manual
Recovery: oligonucleotides dsDNA Recovery: oligonucleotides, dsDNA
Removal <10mers 17–40mers dye terminator proteins Removal <40mers
Sample type: applications DNA, oligonucleotides: Enzymatic reactions
Technology Silica technology

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Kit Handbooks (2)
For MinElute PCR Purification Kit, MinElute Gel Extraction Kit, MinElute Reaction Cleanup Kit
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MinElute PCR Purification Kit
低溶出量でのPCR産物(70 bp~ 4 kb)精製用

MinElute Gel Extraction Kit
低溶出量でのDNAフラグメント(70 bp~ 4 kb)のゲル抽出

MinElute Reaction Cleanup Kit
酵素反応液からのDNA(70 bp~ 4 kb)クリーンアップ用
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