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QIAamp DNA Micro Kit

For purification of genomic and mitochondrial DNA from small samples
  • Rapid purification of high-quality DNA
  • No organic extraction or alcohol precipitation
  • Consistent, high yields
  • Complete removal of contaminants and inhibitors

The QIAamp DNA Micro Kit simplifies purification of genomic and mitochondrial DNA with a fast spin-column procedure. Optimized protocols are included for use with small amounts of fresh or frozen blood, tissue, and dried blood spots. The kit combines the selective binding properties of a silica-based membrane with flexible elution volumes of between 20 and 100 μl. Purification of DNA using the QIAamp DNA Micro Kit can be automated on the QIAcube.

Cat No./ID: 56304
QIAamp DNA Micro Kit (50)
$305.00
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For 50 DNA preps: 50 QIAamp MinElute Columns, Proteinase K, Carrier RNA, Buffers, Collection Tubes (2 ml)
The QIAamp DNA Micro Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
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QIAamp DNA Micro procedure.

Optimized buffers lyse samples, stabilize nucleic acids, and enhance selective DNA adsorption to the QIAamp membrane. Alcohol is added and lysates loaded onto the QIAamp MinElute spin column. Wash buffers are used to remove impurities and pure, ready-to-use DNA is then eluted in water or low-salt buffer.

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Laser microdissection PCR.
DNA was purified from rodent kidney laser microdissection (LMD) samples using the QIAamp DNA Micro Kit or using a direct lysis PCR protocol, as indicated. GAPDH PCR was performed on 1 and 10 µl of each eluate. M: Marker; +: positive control; : negative control.
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Efficient purification of DNA from small sample sizes.
DNA was purified from blood samples (164 µl) using the QIAamp DNA Micro Kit following the blood protocol. DNA was eluted in 20 µl Buffer AE and 2 µl of each eluate was amplified by real-time PCR. Efficient amplification was shown in all cases.
Performance
The resulting purified DNA is free of proteins, nucleases, and other impurities, and is suitable for use in sensitive downstream applications, such as real-time PCR (see figure "Efficient purification of DNA from small sample sizes") and laser microdissection (LMD) PCR (see figure "Laser microdissection PCR"). DNA purified with the QIAamp DNA Micro Kit may also be used in short-tandem repeat (STR) genotyping, single-nucleotide pylomorphism (SNP) genotyping, or pharmacogenomic research.
Principle

The QIAamp DNA Micro Kit combines the selective binding properties of a silica-based membrane with flexible elution volumes of between 20 and 100 µl. DNA binds specifically to the QIAamp MinElute silica-gel membrane while contaminants pass through. No phenol–chloroform extraction is required. PCR inhibitors, such as divalent cations and proteins, are completely removed in two efficient wash steps, leaving pure DNA to be eluted in either water or a buffer provided with the kit. QIAamp DNA Micro technology yields genomic and mitochondrial DNA from small samples ready to use in PCR and blotting procedures.

QIAamp sample preparation technology is fully licensed, allowing QIAamp purified nucleic acids to be used in any molecular assay or other downstream application without risk of patent infringement.

Procedure
The QIAamp DNA Micro Kit simplifies isolation of DNA from small samples with a fast spin-column procedure (see flowchart "Procedure"). The QIAamp DNA Micro Kit uses well-established technology for purification of genomic and mitochondrial DNA from small sample volumes or sizes (e.g., 1–100 µl whole blood, blood cards of ~3 mm diameter, urine, or <10 mg tissue or laser microdissected tissue). Fresh or frozen blood, tissue, and dried blood spots can be purified in 30 minutes and eluted in 20–100 µl.
Applications

The QIAamp DNA Micro Kit procedure is suitable for a wide range of sample materials, including:

Small volumes of blood
Blood cards
Urine
Small tissue samples, including laster microdissections
Features
Specifications
Applications Real-time PCR, STR analysis, LMD-PCR
Elution volume 20–100 µl
Format Spin column
Main sample type Whole blood
Processing Manual (centrifugation or vacuum)
Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein Genomic DNA, mitochondrial DNA
Sample amount 1–100 µl
Technology Silica technology
Time per run or per prep 30 minutes
Yield <3 µg

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Kit Handbooks (2)
少量の血液、乾燥血液班、尿、組織、LMD採取した組織からのゲノムDNAの精製 ゲノムDNAのクリーンアップ
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For purification of genomic DNA from small volumes of blood, dried blood spots, swabs, forensic case work samples, chewing gum, urine, tissues, laser-microdissected tissues; For cleanup of genomic DNA
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