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QIAamp DNA Blood BioRobot MDx Kit

For automated purification of genomic and mitochondrial DNA from whole blood

  • Rapid isolation of high-quality, ready-to-use DNA
  • No organic extraction or alcohol precipitation
  • Consistent, high yields
  • Removal of contaminants and inhibitors

The QIAamp DNA Blood BioRobot MDx Kit provides automated DNA purification on the BioRobot MDx workstation or the BioRobot Universal System using proven QIAamp silica-membrane technology. The fully automated procedure on the BioRobot MDx workstation requires less than 2.5 hours, including bar code reading and complete process documentation, with no hands-on time during the run.

Cat No./ID: 965152
QIAamp DNA Blood BioRobot MDx Kit (12)
$3,431.00
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For 12 x 96 DNA preps: 12 QIAamp 96 Plates, Buffers (Wash Buffers have bar code labels), QIAGEN Protease, Elution Microtubes CL, Caps, S-Blocks, Tape Pad
The QIAamp DNA Blood BioRobot MDx Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
0
QIAamp DNA Blood MDx protocol.
1
No PCR inhibition.
A 1.2 kb fragment of the single-copy Hugl gene was amplified from DNA purified with the QIAamp DNA Blood BioRobot MDx Kit, using HotStarTaq DNA Polymerase in a 50 µl reaction volume. No inhibition was observed using either  A  1 µl of eluate or  B  20 µl of eluate as template.
(Data generated by QIAGEN R&D.)
2
Reliable real-time PCR.

The QIAamp DNA Blood BioRobot MDx Kit was used to purify genomic DNA from 96 whole blood samples (each 200 µl) collected from a single donor. The β-actin gene was amplified from the purified DNA using the TaqMan system. An amplification plot of 96 PCR reactions is shown. (Data generated by QIAGEN R&D.)

Performance

The QIAamp DNA Blood BioRobot MDx Kit process provides DNA isolation on a 96-well plate, automating processing of 32 to 96 samples (200 µl sample volume) in increments of 8 on the BioRobot MDx or the BioRobot Universal System.  The fully automated procedure, including bar code reading, 20-minute load check, and complete process documentation, requires less than 2.5 hours for 96 samples, with no hands-on time. The typical yield is 3–6 µg per 200 µl healthy whole blood, with an elution volume of 200 µl.

The resulting DNA performs well in downstream amplification and Southern blotting assays (see figures "No PCR inhibition" and "Reliable real-time PCR").

Principle

The QIAamp DNA Blood BioRobot MDx Kit simplifies isolation of DNA from whole blood with a fast, automated 96-well–plate procedure. No phenol–chloroform extraction is required. DNA binds specifically to the QIAamp silica-gel membrane while contaminants pass through. PCR inhibitors, such as divalent cations and proteins, are completely removed in three efficient wash steps, leaving pure DNA to be eluted in either water or a buffer provided with the kit. QIAamp technology yields genomic and mitochondrial DNA from whole blood ready to use in PCR and blotting procedures.

The advantages of the fully automated system include high process reliability and walkaway automation, to provide a highly efficient system for DNA purification for laboratories. QIAamp sample preparation technology is fully licensed.

Procedure
Optimized buffers lyse samples, stabilize nucleic acids, and enhance selective nucleic acid adsorption to the QIAamp membrane. Alcohol is added and lysates loaded onto the QIAamp 96-well plate. Wash buffers and ethanol are used to remove impurities, and pure, ready-to-use DNA is then eluted in water or low-salt buffer (see flowchart "protocol").
Applications

The QIAamp DNA Blood BioRobot MDx Kit offers a 96-well format for processesing 32–96 samples in increments of 8. The kit can process samples from whole blood or buffy coat with common anticoagulants such as citrate and EDTA. The QIAamp DNA Blood BioRobot MDx Kit can process blood and buffy coat samples that are fresh or frozen, provided they have not been frozen and thawed more than once. QIAamp technology yields genomic and mitochondrial DNA from whole blood that is ready to use in PCR and blotting procedures.

Features
Specifications
Applications PCR, blotting
Elution volume 200 µl
For automated processing BioRobot MDx
Format 96-well plate
Main sample type Whole blood, buffy coat
Processing Automated
Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein Genomic DNA, mitochondrial DNA
Sample amount 200 µl
Technology Silica technology
Time per run or per prep <2.5 hours
Yield 3–6 µg
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