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DNeasy PowerClean Pro CleanUp Kit

For the removal of PCR inhibitors from purified DNA in just 7 minutes

  • Faster cleanup of problematic DNA in 7 minutes with efficient secondary protocol
  • Efficient purification of nucleic acids from any sample source containing challenging impurities
  • Ready-to-use, high-purity DNA for downstream applications

Clean up problematic DNA in a snap. Using the streamlined DNeasy PowerClean Pro CleanUp Kit’s 7-minute protocol and Inhibitor Removal Technology provides researchers with a novel and proprietary method for cleaning up previously isolated genomic DNA. Regardless of whether the inhibiting substance colors previously processed DNA or not, the DNeasy PowerClean Pro CleanUp Kit will remove both color and inhibitors, such as humic acids, heme, polysaccharides, polyphenols, fluvic acids, lipids and dyes from samples. Resultant, high-purity DNA is ready to use in all downstream applications including qPCR and next-generation sequencing.

The DNeasy PowerClean Pro CleanUp Kit performs well on DNA from any sample source. DNA samples are combined with propriety DNA cleanup reagents to remove inhibitors from the DNA solutions. Using a silica membrane spin column, all DNA, including total genomic DNA is captured. Washing and eluting the DNA from the spin column provides the purified DNA, ready for downstream applications. Percentage recovery varies depending on the level of inhibitors in the DNA that may be influencing the DNA yield measurement. We recommend quantifying DNA via a PicoGreen assay or running DNA on an agarose gel to determine recovery yield.

DNeasy PowerClean Pro CleanUp Kit was formally sold by MO BIO as PowerClean Pro DNA Clean-Up Kit.

Cat No./ID: 12997-50
DNeasy PowerClean Pro Clean Up Kit (50)
$372.00
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For the removal of PCR inhibitors from purified DNA in just 7 minutes
The DNeasy PowerClean Pro CleanUp Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
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Figure 1. Elimination of humic substances from DNA.
Samples cleaned up with either the DNeasy PowerClean Pro Clean-Up Kit or the Zymo Research OneStep PCR Inhibitor Removal Kit were examined on a 1.2% TAE gel (A). Following clean-up with the PowerClean Pro DNA Kit, the samples were clear. However, samples processed with the OneStep Kit retained some brown color, indictaing that not all inhibitors were removed (B). The concentration of the starting sample was observed to be artificially high, with low 260/280 and 260/230 ratios, indicating the presence of contaminants that absorb at A230, such as humic substances. Following clean-up with the PowerClean Pro DNA Kit, the 260/280 and 260/230 ratios increased to levels consistent with pure DNA and the concentration of the DNA decreased to an average of 76.85 ng/μl, a value that was confirmed by quantitation using a Qubit PicoGreen Assay (data not shown). For samples cleaned-up with the OneStep Kit, the 260/280 and 260/230 ratios remained low, indicating that inhibitors were still present. Starting samples were brown due to the presence of humic substances.
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Figure 2. Successful qPCR amplification.
DNA described in Fig. 1 was examined via qPCR with a Bacillus 16s assay (1 µl of undiluted DNA cleaned up using two different methods, or 1:10 dilution). Samples cleaned up with the DNeasy PowerClean Pro CleanUp Kit were free of inhibitors, as indicated by successful amplification and a difference of approximately 3 cycles between the undiluted DNA and the 1:10 dilution. Undiluted samples cleaned up with the Zymo OneStep Kit failed to amplify, and 1:10 dilutions amplified with a higher Cq value than the DNeasy PowerClean Pro CleanUp Kit 1:10 dilution, indicating that PCR inhibitors remained in the samples.
Performance


Features
Specifications
Binding capacity Up to 20 µg per prep
Format Silica Spin Filter
Sample size Up to 100 µl of purified DNA
Sample types Processed previously purified DNA
Size range 60 bp - 50 kb
Storage temperature Store at room temperature (15-30°C)
Throughput 1-24 samples
Time per run or per prep 7 minutes
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